Vibrio alginolyticus bacteriophage microecological preparation as well as preparation method and application thereof
A micro-ecological preparation and technology of Vibrio alginolyticus, applied in the field of microorganisms, can solve problems such as low phage titer, and achieve the effects of increasing titer, prolonging fermentation time, and eliminating content
Inactive Publication Date: 2018-06-01
XIAMEN CANCO BIOTECH CO LTD
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The invention provides a vibrio alginolyticus bacteriophage microecological preparation as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The preparation method comprises the following steps: inoculating a vibrio alginolyticus solution and a vibrio alginolyticus bacteriophage solution into a fermental cultivation solution for fermental cultivation; performing the fermental cultivation for 6 to 15 h, then adding the vibrio alginolyticus solution into a host fermentation solution in a flowing manner for continuous fermental cultivation, controlling the whole fermentation period at 18 to 24 h, and inactivating vibrio alginolyticus hosts in the fermented solution, thus obtaining the vibrio alginolyticus bacteriophage microecologicalpreparation. According to the preparation method provided by the invention, fermentation and flowing adding moments are controlled to perform the fermental cultivation for 6 to 15 h, and then the hostsolution is added in the flowing manner, so that the titer of vibrio alginolyticus bacteriophage can be greatly improved without prolonging the fermentation time.
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