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Acidovorax wautersii TCP2011036 and application thereof

A technology of TCP2011036 and Acidovorax worriens, applied in Acidovorax worriori TCP2011036 and its application fields, can solve the problems of few microbial resources and weak research foundation, and achieve the goal of promoting plant growth, reducing use, and inhibiting plant diseases Effect

Active Publication Date: 2018-06-08
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the foundation of research work on microbial resources in our region is still relatively weak, many works are still in their infancy, and there are few microbial resources available.

Method used

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  • Acidovorax wautersii TCP2011036 and application thereof
  • Acidovorax wautersii TCP2011036 and application thereof
  • Acidovorax wautersii TCP2011036 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Isolation and identification of strains

[0028] 1. Isolation of strains

[0029] The test material: the stem base tissue of the sugarcane variety material CP85-1508 planted in the suburbs of Nanning, Guangxi Zhuang Autonomous Region.

[0030] Take the sugarcane stems, soak them in 70% alcohol for 30 seconds, wash them with sterile water three times, then soak them in 0.1% mercury chloride for 1 minute, soak them in 70% alcohol for 30 seconds, and wash them with sterile water five times. Weigh 1g of the surface-sterilized tissue block, soak it in 10mL of phosphate buffer, grind it in a sterile mortar, and let it stand for 5min. Take the supernatant and make a 10-fold serial dilution with sterile water, and take 100μL of the original solution and the diluted solution respectively. Spread on nitrogen-free enrichment medium (the composition is glucose 10.0g, KH 2 PO 4 0.2g, MgSO 4 ·7H 2 O 0.2g, NaCl 0.2g, CaSO 4 2H 2 O 0.2g, CaCO 3 5.0g, 14.0g agar, 1000mL deioni...

Embodiment 2

[0041] Plant Growth-promoting Properties of Acidovorax worriori Strain TCP2011036

[0042] (1) Determination of the ability of the strain to secrete IAA

[0043] Make a standard curve of pure IAA, PC colorimetric solution and S2 colorimetric solution

[0044] Acidovorax worthii TCP2011036 strain was inoculated in NB liquid medium for 16 hours (28°C, 120r / min), and the OD600 value was adjusted to 0.05 with sterile water, and 100 μL of the suspension of Acidovorax worii TCP2011036 was inoculated respectively In the King culture medium without tryptophan (the composition is peptone 20g, dipotassium hydrogen phosphate 1.5g, magnesium sulfate 1.5g, distilled water 1000mL, pH 7.0) and King culture medium containing 100mg / L tryptophan, repeat 4 The second time, the culture solution added with 100 μL of sterile water was used as a blank control, and they were placed together in a temperature-controlled shaker at 28°C with a rotation speed of 120r / min for 10 days. Centrifuge the bact...

Embodiment 3

[0050] Control Effect of Acidovorax worriii TCP2011036 Strain on Pepper Bacteria Wilt

[0051] The pepper seedlings with a seedling age of 40 days were transplanted into the soil containing the capsicum solanacearum, and each pot was planted as a single plant. After transplanting, each pot was inoculated with 50 mL of fermentation broth (inoculated with Acidovorax TCP2011036) cultured in NB culture medium for 48 hours. TCP2011036 group), the control was inoculated with the same amount of sterile culture medium (CK group), and then inoculated with the same amount of fermentation broth once after 7 days, and the incidence of pepper was continuously observed and recorded after inoculation. The results showed that the pepper inoculated with acidovorax TCP2011036 fermented liquid grew well, and the control effect of the bacterial strain on bacterial wilt of pepper reached 82.8%, and the growth of pepper was significantly promoted, such as image 3 and shown in Table 1.

[0052] Th...

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Abstract

The invention discloses Acidovorax wautersii TCP2011036. The classification name of the Acidovorax wautersii TCP2011036 is Acidovorax wautersii TCP2011036, the 16S rDNA gene sequence list of the strain is SEQ ID NO.1 as shown in the specification, the preservation institute is the China General Microbiological Culture Collection Center, the preservation date is June 19, 2017, and the preservationnumber is CGMCC No.14252. The strain disclosed by the invention has a remarkable promotion function on growth of sugarcane seedlings, and the strain can be applied to production of microbial agents and biological organic fertilizers for promoting growth of plants and inhibiting insects and diseases of plants, has great significances for reducing chemical pesticides and protecting ecological environments, and has wide application prospects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain of acidovorax TCP2011036 and its application. Background technique [0002] Every year, the world's field crops are reduced by 20% due to diseases, and severe damage can cause 50% losses, or even crop failure. At present, the main means of controlling plant diseases is to use chemical fungicides, but their residual toxicity in crops and in the environment is potentially dangerous to human health, and has become a concern of the whole society. With the continuous development and progress of science and technology, the call for reducing the use of chemical pesticides and protecting the living environment of human beings is increasing day by day. In order to protect the environment, maintain ecological balance and human health and safety, the development and use of agricultural microbial resources with biocontrol functions has become a direction of modern agricultural developm...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P21/00C05F11/08C12R1/01
CPCA01N63/00C05F11/08C12N1/205C12R2001/01
Inventor 胡春锦史国英曾泉农泽梅岑贞陆叶雪莲
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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