Method for selectively preparing ginsenoside rd from saponins of ginseng by enzymatic method
A technology of ginsenosides and ginsenosides, applied in biochemical equipment and methods, enzymes, hydrolytic enzymes, etc., can solve the problems of only increasing the price of ginsenosides, limiting the large-scale use of ginsenosides, consumption costs, time, etc.
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reference example 1
[0035] [Reference Example 1] Preparation of ginseng refined saponins
[0036] 2 kg of red ginseng, white ginseng, fresh ginseng, ginseng whiskers, or their ginseng leaves, ginseng flowers, and ginseng fruits were put into 20 L of ethanol, extracted three times by reflux, and settled at 15°C for 6 days. After that, the residue and the filtrate were separated by filter cloth filtration and centrifugation. The concentrated solution obtained by concentrating the separated filtrate under reduced pressure was suspended in water, and extracted with 1 L of ether for 5 times to remove the pigment. -Butanol extraction 3 times. All the 1-butanol layers thus obtained were treated with 5% KOH, washed with distilled water, and then concentrated under reduced pressure to obtain a 1-butanol concentrate, which was dissolved in a small amount of methanol, and then a large amount of ethyl acetate was added. 40-80 g of purified ginseng saponins (containing ginsenosides Rb1, Rb2, Rc, Rd, Re, Rg1,...
Embodiment 1
[0037] [Example 1] Preparation of ginsenoside Rd by enzymatic reaction
[0038] 10 g of the purified ginseng saponins (containing ginsenosides Rb1, Rb2, Rc, Rd, Re, Rg1, Rf, etc.) in the above Reference Example 1 were dissolved in 1 L of water.
[0039] Then, 200% by weight of the pectinase isolated from Trichoderma reesei with respect to the substrate was added to the above-mentioned mixed solution, followed by reaction at 30°C for 48 hours. Complete consumption of the substrate was periodically confirmed by thin layer chromatography, and the reaction was terminated by heating in a boiling water bath for 10 minutes to inactivate the enzyme. Finally, ethyl acetate or ethanol was added to the reaction solution at a ratio of 1:1 (to the volume ratio of the reaction solution), extracted three times, concentrated, and then separated by silica gel column chromatography (chloroform:methanol=9:1) Ginsenoside Rd ( figure 1 ).
[0040] In 10 g of the ginseng refined saponins in Refe...
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