Preparation method of diabetes vaccine based on (M)T-DC technology

Abstract

The invention relates to a preparation method of diabetes vaccine based on a (M)T-DC technology. The preparation method selects a specific single-epitope multiple antigen peptide in-vitro sensitization dendritic cell to obtain an auto-dendritic cell preparation serving as venous re-transfusion diabetes vaccine. According to the preparation method, the (M)T-DC technology is adopted to interfere andremove a blood vessel endothelium infectious factor, the vaccine can deeply enter a lesion local part in a targeting manner, the blood vessel endothelium is restored by combining multi-direction differentiation potential mesenchymal stem cell therapy of a corresponding treatment course, cicatrix is dissolved, the pancreatic tissue is restored, the pancreatic immunity system is restored, and an insulin beta cell function is recovered, so that the insulin can secrete normally, thereby achieving a treatment purpose.

Description

technical field [0001] The invention relates to a vaccine, in particular to a preparation method of a diabetes vaccine based on (M)T-DC technology. Background technique [0002] Diabetes mellitus is a metabolic disease characterized by hyperglycemia due to defects in insulin secretion or insulin β-cell action. Sustained high blood sugar and long-term metabolic disorders can lead to damage, dysfunction and failure of tissues and organs throughout the body, especially the eyes, kidneys, cardiovascular and nervous systems. Severe cases can cause acute complications such as dehydration, electrolyte disturbance and acid-base imbalance, ketoacidosis and hyperosmolar coma. Drugs and diet are used in treatment, but it is difficult to achieve the desired effect. [0003] According to research, cytotoxin-associated protein (CagA), vacuolar toxin-associated protein (VacA), urease (Ure), cytomegalovirus (CMV), human papillomavirus (HPV-16), human papillomavirus (HPV- 18), herpes simp...

AI Technical Summary

Problems solved by technology

Long-term low-virulence infection will cause the immune system to gradually adapt to this infection. In the case of low immunity, the corresponding immune response cannot be caused against it, resulting in immune tolerance. The body cannot recognize the relevant pathogenic microorganisms, which will lead to pathogenic microorganisms. Long-term persistent infection, causing long-term damage to the body

[0004] This type of infection will cause serious pathological damage to the cardiovascular and cerebrovascular, and the continuous damage of vascular endothelial cells will cause diseases of the vascular system; the destruction of the vascular endothelium will form microthrombi, and the formation of microthrombi will block capillaries, resulting in the slow function of vascular cells. Regression, the progressive damage of blood vessels slowly accumulates, from quantitative deformation to qualitative change, once a breakthrough occurs, the damage to organ function is irreversible; it will lead to a decline in the function of the blood supply system, and damage to blood vessel function will lead to the occurrence of diseases such as diabetes

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