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Polyurethane micro-carrier as well as preparation method and application thereof

A technology of polyurethane microspheres and microcarriers, which is applied in the preparation of microspheres, microcapsule preparations, prostheses, etc., can solve problems such as lack of injectability, poor mechanical properties, and large limitations, and achieve good clinical application prospects, The effect of less environmental pollution and low cost

Active Publication Date: 2018-06-12
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the most widely used microcarrier material is gelatin, which can effectively cultivate cells, but because it is a natural polymer material, its mechanical properties are very poor, and it has great limitations for in vivo repair.
[0005] The mechanical properties of artificial synthetic materials are usually relatively good, but it is difficult to meet the two conditions of efficient cell culture and injectable performance
For example, among polyurethane materials, the currently reported ones that can be used as microcarriers are polyurethane foam materials, which do not have injectable properties and are very limited for in vivo repair.

Method used

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  • Polyurethane micro-carrier as well as preparation method and application thereof
  • Polyurethane micro-carrier as well as preparation method and application thereof
  • Polyurethane micro-carrier as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1 The preparation of polyurethane microspheres of the present invention

[0074] 1. Preparation method

[0075] The preparation method of cell-loaded polyurethane microspheres comprises the following steps:

[0076] (1) Oligomer diol premix

[0077] Add polycaprolactone diol 1000 and PEG200 into the three-necked flask, the molar ratio of the two is 1:1, stir and mix at 70°C;

[0078] (2) Prepolymerization reaction:

[0079] Isophorone diisocyanate and dihydric alcohol in step (1) are raw materials, added in the reaction vessel, stirred at a speed of 300rmp, and reacted for 2h;

[0080] The mol ratio of isophorone isocyanate to total oligomer diol is 2:1;

[0081] (3) Chain extension reaction

[0082] Add 2,2-dimethylolpropionic acid after the above step (2), cool down to 45°C, and react for 2 hours at a stirring speed of 700rmp;

[0083] Wherein the mol ratio of chain extender and isocyanate in step (2) is 0.1:1;

[0084] (4) neutralization:

[0085] ...

Embodiment 2

[0091] The preparation of embodiment 2 polyurethane microspheres of the present invention

[0092] 1. Preparation method

[0093] The preparation method of cell-loaded polyurethane microspheres comprises the following steps:

[0094] (1) Oligomer diol premix

[0095] Add polytetrahydrofuran and PEG200 into a three-necked flask, the molar ratio of the two is 1.5:1, stir and mix at 70°C;

[0096] (2) Prepolymerization reaction:

[0097] Isophorone diisocyanate and dihydric alcohol in step (1) are raw materials, added in the reaction vessel, stirred at a speed of 700rmp, and reacted for 3h;

[0098] The mol ratio of isophorone isocyanate and total oligomer glycol is 2.5:1;

[0099] (3) Chain extension reaction

[0100] Add 2,2-dimethylolpropionic acid after the above step (2), cool down to 50°C, and react for 3 hours at a stirring speed of 300rmp;

[0101] Wherein the mol ratio of chain extender and isocyanate in step (2) is 1:1;

[0102] (4) neutralization:

[0103] Add ...

Embodiment 3

[0109] The preparation of embodiment 3 polyurethane microspheres of the present invention

[0110] 1. Preparation method

[0111] The preparation method of cell-loaded polyurethane microspheres comprises the following steps:

[0112] (1) Oligomer diol premix

[0113] Add polycaprolactone diol 1000 and PEG200 into a three-necked flask, the molar ratio of the two is 2:1, stir and mix at 70°C;

[0114] (2) Prepolymerization reaction:

[0115] Isophorone diisocyanate and dibasic alcohol in step (1) are raw materials, added in the reaction vessel, stirred at a speed of 380rmp, and reacted for 4h;

[0116] The mol ratio of isophorone isocyanate and total oligomer glycol is 2.5:1;

[0117] (3) Chain extension reaction

[0118] After the above step (2), add 2,2-dimethylolpropionic acid, while cooling down to 55°C, and react at a stirring speed of 380rmp for 2h;

[0119] Wherein the mol ratio of chain extender and isocyanate in step (2) is 1:1;

[0120] (4) neutralization:

[0...

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Abstract

The invention discloses a polyurethane microsphere of which the particle size is 150 to 270mu m. The invention also discloses a preparation method and application of the polyurethane microsphere. Thepolyurethane microsphere prepared by the preparation method disclosed by the invention can be used as a micro-carrier, so that high-yield cells can be obtained; meanwhile, the polyurethane microspherehas the advantages of good biocompatibility, capabilities of being injected and being used in vivo repair and better application prospects.

Description

technical field [0001] The invention relates to a polyurethane microcarrier and its preparation method and application, belonging to the field of biological materials. Background technique [0002] Microcarriers refer to microbeads with a diameter of usually 60-300 μm, which are suitable for the growth of anchorage-dependent cells on their surfaces. Cultured cells on microcarriers have unique advantages: they have the surface required for anchorage-dependent cell growth; due to their large surface area / volume ratio, they enable a scalable, homogeneous culture system that allows cells to grow on a small amount of material Adhesion and large-scale expansion are achieved; the expanded cells are easy to aggregate on the surface of the microcarrier to form a complex, which promotes the interaction between cells; the secreted extracellular matrix also supports intracellular activities, both of which can promote cell proliferation. [0003] Current microcarriers are commonly used...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G18/75C08G18/66C08G18/42C08G18/32C08G18/12C08G18/34A61K47/34A61L27/18A61L27/50
CPCA61K47/34A61L27/18A61L27/50A61L2300/258A61L2300/412A61L2400/06C08G18/12C08G18/3206C08G18/4277C08G18/664C08G18/755C08L75/06C08G18/348A61L27/38C08G18/0823C08G18/4018C08G18/4808C08G18/4833C08G18/4854C08L75/04B01J13/14C08G8/10
Inventor 解慧琪董丽龚梅陈安静
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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