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Microbial preparation for treatment of industrial sewage, preparation method and application thereof

A technology of microbial preparations and industrial sewage, applied in special treatment targets, chemical instruments and methods, biological water/sewage treatment, etc., can solve the problems of residents' health, negative impact on safety, water pollution, etc.

Inactive Publication Date: 2018-06-15
江苏世邦生物工程科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large amount of domestic and industrial sewage flowing into rivers, lakes or groundwater will cause serious pollution to the water body and negatively affect the health and safety of residents

Method used

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  • Microbial preparation for treatment of industrial sewage, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The microbial preparation for treating industrial sewage consists of particles with a core-shell structure. The particles with a core-shell structure include a composite preparation, a middle shell, and an outer shell arranged sequentially from the inside to the outside; the composite preparation includes Bacillus amyloliquefaciens BMF01 Fermentation broth and immobilized lipase. In the microbial preparation, the weight ratio of the composite preparation, the middle shell layer and the outer shell layer is 1:0.6:0.8.

[0037] The preparation method of above-mentioned microbial preparation is:

[0038] 1) Cultivate activated marine Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) BMF01 to the logarithmic phase, and then inoculate the strains cultivated to the logarithmic phase into LB liquid medium according to the inoculum volume of 5% of the medium volume, at 25°C Under 180rpm shaking table, cultivated for 24 hours to prepare marine Bacillus amyloliquefaciens (...

Embodiment 2

[0045] The microbial preparation for treating industrial sewage consists of particles with a core-shell structure. The particles with a core-shell structure include a composite preparation, a middle shell, and an outer shell arranged sequentially from the inside to the outside; the composite preparation includes Bacillus amyloliquefaciens BMF01 Fermentation broth and immobilized lipase. In the microbial preparation, the weight ratio of the composite preparation, the middle shell layer and the outer shell layer is 1:0.5:1.

[0046] The preparation method of above-mentioned microbial preparation is:

[0047] 1) Cultivate activated marine Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) BMF01 to the logarithmic phase, and then inoculate the strains cultivated to the logarithmic phase into LB liquid medium at an inoculum volume of 2% of the medium volume, at 25°C Next, the shaker was shaken at 120 rpm, and cultured for 48 hours to prepare the fermentation broth of Bacillus...

Embodiment 3

[0068] Take the microbial preparations prepared in Examples 1-2 and Comparative Examples 1-2, and test their influence on various indicators of the water body.

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Abstract

The invention discloses a microbial preparation for treatment of industrial sewage. The microbial preparation is composed of core-shell structure particles, which consist of a compound preparation, anintermediate shell layer, and an outer shell layer that are arranged from the inside out in order. The compound preparation includes the marine Bacillus amyloliquefaciens BMF01 fermentation broth andimmobilized lipase, and the immobilized lipase is obtained by immobilization of lipase on the surfaces of amino functionalized Al2O3 nanoparticles. The intermediate shell layer is a shell composed ofa plant glue. The outer shell layer is composed of inorganic gel, calcium chloride and calcium acetate. The invention also discloses a preparation method and application of the microbial preparation.The microbial preparation provided by the invention can achieve efficient treatment of industrial wastewater to substantially remove organic matters, ammonia nitrogen and heavy metals from industrialwastewater.

Description

technical field [0001] The invention relates to the field of industrial sewage treatment, in particular to a microbial preparation for treating industrial sewage and its preparation method and application. Background technique [0002] With the advancement of urbanization and the rapid development of industry, the problem of environmental pollution is becoming more and more serious. A large amount of domestic and industrial sewage flowing into rivers, lakes or groundwater will cause serious pollution to water bodies and negatively affect the health and safety of residents. [0003] At present, sewage treatment technologies mainly include the following: (1) adding chemical substances to sewage to degrade or recover pollutants in sewage, including precipitation, neutralization, oxidation-reduction and chemical-physical disinfection; (2) )Using physical and chemical methods to remove pollutants in sewage, including ion exchange, adsorption and membrane separation; (3) Separati...

Claims

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Application Information

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IPC IPC(8): C02F3/34
CPCC02F3/341C02F3/342C02F2303/02
Inventor 谢悦波姚竣耀刘才群
Owner 江苏世邦生物工程科技有限公司
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