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Rapid germination culture medium for primary culture of ascendens mucronatum

A germination medium and primary culture technology, applied in horticulture, botanical equipment and methods, horticultural tools/equipment, etc., can solve the problems that restrict the popularization and planting of Chinese fir, the limited number of seed strips, and the low survival rate of cuttings, and achieve induction The effect is good, the germination rate is fast, and the effect of improving the reproduction efficiency

Active Publication Date: 2018-06-22
JIANGSU WUJIN HIGHWAY PLANT NURSERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main method of propagating Zhongshan fir seedlings is cutting, which can keep the seedlings to maintain the excellent characteristics of the mother tree, but Zhongshan fir seedlings are lignified earlier, the survival rate of cuttings is low, and the number of seedlings is limited, the speed is slow, and the seedling production The rate is low, which restricts the large-scale popularization and planting of Zhongshan fir

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A fast germination medium for primary culture of Zhongshan fir, including an induction medium and a strong seedling medium;

[0023] The formulation of the induction medium is 2 / 3MS medium+3mg / L 2,4-D+5mg / L NAA+5mg / L banana puree+0.01mg / L TDZ+2mg / L 6~ BA+10g / L agar+0.02 organic matter+4g sucrose+2mg / L camphor oil; wherein the organic matter is a mixture of glutathione reductase and cysteine, and the glutathione reductase The mass ratio to cysteine ​​is 2:1.

[0024] The formula of the strong seedling medium is 1 / 2MS medium+10mg / L sodium nitrophenolate+10mg / L ascorbic acid+5mg / L GA+6mg / L IAA+0.03mg / L PVP+10mg / L of activated carbon powder + 2g / L of sucrose + 10g / L of agar.

Embodiment 2

[0026] A fast germination medium for primary culture of Zhongshan fir, including an induction medium and a strong seedling medium;

[0027] The formulation of the induction medium is 2 / 3MS medium+5mg / L 2,4-D+7mg / L NAA+10mg / L banana puree+0.05mg / L TDZ+4mg / L 6~ BA+20g / L agar+0.06 organic matter+6g sucrose+3mg / L camphor oil; wherein the organic matter is a mixture of glutathione reductase and cysteine, and the glutathione reductase The mass ratio to cysteine ​​is 2:1.

[0028] The formula of the strong seedling medium is 1 / 2MS medium+30mg / L sodium nitrophenolate+30mg / L ascorbic acid+10mg / L GA+10mg / L IAA+0.06mg / L PVP+20mg / L of activated carbon powder + 4g / L of sucrose + 20g / L of agar.

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Abstract

The invention relates to a rapid germination culture medium for primary culture of ascendens mucronatum. The culture medium comprises an induction medium and a strong seedling culture medium; the culture medium contains abundant nutrient ingredients and is suitable for multiplication and rapid germination of ascendens mucronatum, the ascendens mucronatum culture period is greatly shortened, and the production cost is lowered; the germination rate of an explant of primary culture of ascendens mucronatum exceeds 98%, the average germination time is shorter than 18 d, and the germination speed ishigh; the browning rate of the explant during tissue culture can be effectively lowered, the survival rate of the explant of ascendens mucronatum is increased, and the multiplication efficiency is improved.

Description

Technical field [0001] The invention relates to the technical field of tissue culture of Zhongshan fir, in particular to a rapid germination medium for the primary culture of Zhongshan fir. Background technique [0002] Zhongshan cedar is a semi-evergreen tall tree with a tall trunk and beautiful tree shape. It is an excellent interspecific hybrid of three species of Taxodium chinense, Taxodium chinense, and Taxodium chinense. It was researched by the Institute of Botany, Chinese Academy of Sciences, Jiangsu Province after years of experimentation. Breeding. It has the characteristics of long green period, fast growth, water and humidity resistance, salt and alkali resistance, strong wind resistance, less pests and diseases, wide adaptability, rapid growth, and superior quality than its parents. It is not only an excellent tree species for farmland forest network and tidal flat afforestation, but also presents broad development prospects in urban landscaping, water conservation,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 谢健仇玉成许丽群王婷俞佳丽潘海峰俞璟武洁洁沈卿周奕成
Owner JIANGSU WUJIN HIGHWAY PLANT NURSERY
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