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A method for increasing the biomass of Chlamydomonas and promoting the lipid production of Chlamydomonas

A technology of Chlamydomonas lipids and Chlamydomonas, applied in the field of microorganisms, can solve the problems of low lipid production efficiency and accumulation

Active Publication Date: 2021-09-28
SHANGHAI NORMAL UNIVERSITY
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Problems solved by technology

Like other microalgae, the growth of Chlamydomonas reinhardtii was inhibited under nitrogen deficiency, i.e. algal biomass and lipid accumulation were inversely correlated with nitrogen deficiency, resulting in its lipid production efficiency and accumulation below theoretical values

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  • A method for increasing the biomass of Chlamydomonas and promoting the lipid production of Chlamydomonas
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  • A method for increasing the biomass of Chlamydomonas and promoting the lipid production of Chlamydomonas

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Embodiment Construction

[0053] Before the present invention is described, it is to be understood that this invention is not limited to the particular methods and experimental conditions described, as such methods and conditions may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting, the scope of the present invention being limited only by the appended claims.

[0054] 1. Materials and methods

[0055] 1.1 Culture conditions of algae and bacterial strains

[0056] Chlamydomonas reinhardtii cc849 is a cell-deficient strain. At 25±1°C, at 100μE·m -2 ·s -1 The algae were grown in Tris-acetate phosphate (TAP) medium (pH=7) under the conditions of . The cell density was determined by the absorbance at 750 nm (OD 750 )Sure. Chlorophyll of Chlamydomonas reinhardtii was extracted with alcohol, that is, 1ml algae cells were taken, centrifuged at 8000g for 1min, the supernatant was removed, and...

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Abstract

The invention discloses a method for increasing the biomass of Chlamydomonas under nitrogen deficiency culture conditions, and also discloses a method for promoting lipid production of Chlamydomonas. The method co-cultivates Chlamydomonas and Azotobacter torus, comprising: Step 1, culturing Chlamydomonas in TAP medium; Step 2, cultivating Azotobacter torus in nitrogen fixation medium; Step 3, centrifuging Harvest the Chlamydomonas in step 1, wash and remove nitrogen with TAP-N medium; step 4, after the growth of Azotobacter ureus in step 2 to saturation, harvest Azotobacter ureus by centrifugation, wash with TAP-N medium and repeat overhang to make it OD 600 =1; step 5, mix the Chlamydomonas harvested in step 3 with the Azotobacter terrescens harvested in step 4 in TAP-N medium, and cultivate under light; step 6, harvest the clothes after 1-9 days of mixed culture Algae, lipids were extracted with a chloroform / methanol mixture. Utilizing Azotobacter terrescens to increase the biomass and lipid accumulation of Chlamydomonas is an effective, rapid and economical strategy.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for increasing the biomass of Chlamydomonas and promoting the lipid production of Chlamydomonas under nitrogen-deficiency culture conditions. Background technique [0002] The sources of traditional fossil fuels are limited, and carbon dioxide and other gases are usually produced during use, so it is urgent to find new renewable and clean energy resources. Biodiesel is a type of biofuel that is considered environmentally friendly. Raw material supply and price are the key limiting factors for biodiesel application, so seeking cheap renewable raw materials for biodiesel research is an effective way to achieve large-scale utilization of biodiesel. [0003] Algae can convert carbon dioxide and water into oxygen and macromolecular organic compounds in the form of carbohydrates and lipids through photosynthesis. Under certain stress conditions, such as high light or ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12N1/20C12P7/64C12R1/065C12R1/89
CPCC12N1/12C12N1/20C12P7/6463
Inventor 许丽丽程祥龙涂晓盟曹玥王全喜
Owner SHANGHAI NORMAL UNIVERSITY
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