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A kind of compound microorganism preparation and its application in preventing and treating root-knot nematode

A technology of compound microorganisms and preparations, which is applied in the field of microorganisms, can solve problems such as unsatisfactory control effects and poor synergistic performance of strains, and achieve the effects of simple preparation process, broad application prospects and reasonable compatibility

Active Publication Date: 2021-05-28
山东科力可生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a compound microbial preparation and its application in the prevention and treatment of root-knot nematodes, so as to overcome the defects of unsatisfactory control effect and poor synergistic performance of strains in the prior art compound biocontrol preparation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A composite microbial preparation, which includes Bacillus thuringiensis, Bacillus sphaericus, Arthrobush oligosporum, and Streptomyces hygroscopicus;

[0029] Specifically, it is prepared according to the following process:

[0030] Bacillus thuringiensis seed liquid (1 × 10 8 cfu / ml) and Bacillus sphaericus seed solution (1×10 8 cfu / ml) mixed according to the volume ratio of 1:1, then inserted into the fermentation medium according to the inoculation amount of 8% volume ratio, and cultured at 28°C for 24h to obtain a mixed fermentation broth of Bacillus thuringiensis-Bacillus sphaericus; the fermentation The formula of the medium is: corn starch 10g / L, glucose 8g / L, bean cake powder 5g / L, ammonium sulfate 2g / L, dipotassium hydrogen phosphate 2g / L, potassium dihydrogen phosphate 2g / L, magnesium sulfate 0.1g / L L, manganese sulfate 0.1mg / L;

[0031] A. oligospora seed liquid (1 × 10 7 cfu / ml) was inserted into the fermentation medium according to the inoculation amou...

Embodiment 2

[0037] A composite microbial preparation, which includes Bacillus thuringiensis, Bacillus sphaericus, Arthrobush oligosporum, and Streptomyces hygroscopicus;

[0038] Specifically, it is prepared according to the following process:

[0039] Bacillus thuringiensis seed liquid (1 × 10 8 cfu / ml) and Bacillus sphaericus seed solution (1×10 8 cfu / ml) mixed according to the volume ratio of 1:1, then inserted into the fermentation medium according to the inoculation amount of 8% volume ratio, and cultured at 28°C for 24h to obtain a mixed fermentation broth of Bacillus thuringiensis-Bacillus sphaericus; the fermentation The formula of the medium is: corn starch 10g / L, glucose 8g / L, bean cake powder 5g / L, ammonium sulfate 2g / L, dipotassium hydrogen phosphate 2g / L, potassium dihydrogen phosphate 2g / L, magnesium sulfate 0.1g / L L, manganese sulfate 0.1mg / L;

[0040] A. oligospora seed liquid (1 × 10 7 cfu / ml) was inserted into the fermentation medium according to the inoculation amou...

Embodiment 3

[0046] The operation method of root-knot nematode control experiment

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PUM

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Abstract

The invention belongs to the technical field of microbes, and discloses a composite microbial preparation, which includes bacillus thuringiensis, bacillus sphaericus, Arthrobus sp. oligosporum and streptomyces hygroscopicus. The compound microbial preparation of the invention has good insecticidal effect, can effectively prevent and control root-knot nematodes, and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a compound microbial preparation and its application in preventing and controlling root-knot nematodes. Background technique [0002] Root-knot nematode (Meloidogyne) is a highly specialized omnivorous plant pathogenic nematode. Root-knot nematode is widely distributed all over the world and has a wide range of hosts. It is one of the important pathogens that endanger crops. Root-knot nematode is the second largest soil-borne disease in the world, causing serious economic losses to crops all over the world every year. At present, there are more than 80 kinds of root-knot nematodes reported in the world, and 29 kinds have been reported in my country, mainly parasitic on more than 2000 kinds of hosts such as vegetables, food crops, cash crops, fruit trees, ornamental plants and weeds. Plants are severely damaged, and its harm has surpassed bacteria and viruses,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14A01N63/23A01N63/22A01N63/28A01N63/20A01P5/00C12R1/07C12R1/645C12R1/55
CPCA01N63/10A01N63/30C12N1/14C12N1/20
Inventor 陆平丰朱金华吕奇磊邵全民吕长增
Owner 山东科力可生物技术有限公司