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Method for preparing L-arginine-alpha-oxoglutarate through bio-enzyme method

A technology of ketoglutarate and bio-enzyme method, which is applied in the field of bio-enzyme preparation of L-arginine-α-ketoglutarate, can solve the problems of high risk, high cost, and large production pollution, and achieve improved Effects on product quality, emission reduction, and raw material cost reduction

Active Publication Date: 2018-06-29
SHANDONG YANGCHENG BIOLOGY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The two main raw materials of arginine are arginine and α-ketoglutaric acid. Among them, arginine is easy to obtain because of its mature large-scale production process, but α-ketoglutaric acid is more difficult to obtain.
At present, the preparation methods of α-ketoglutarate mainly include fermentation method and chemical synthesis method. During the production of α-ketoglutarate by fermentation method, there are many by-products such as pyruvic acid, fumaric acid and malic acid, and the fermentation cycle is long. Therefore, it is difficult to obtain high-purity products
At present, α-ketoglutaric acid is mainly produced by chemical methods, but a large amount of highly toxic substances are used in the production, resulting in large production pollution, high risk, complicated process and high cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for preparing L-arginine-alpha-ketoglutarate by a biological enzymatic method, comprising the following steps:

[0029] (1) Preparation of reaction solution

[0030] Add 25m³ of pure water to the 50m³ reactor, add 3000kg of glutamic acid and 3650kg of arginine to adjust the pH of the solution to 6.9, and use pure water to set the volume to 30m³.

[0031] (2) Enzyme reaction

[0032] Add 3.5U / mL glutamate oxidase and 200U / mL catalase to the reaction solution to start the reaction, control the dissolved oxygen to 10%, control the temperature to 39±0.5°C, and control the pH to 6.9. After 10 hours of reaction, use biological The sensor detects that there is no glutamic acid, and 3452kg of arginine is added to obtain the L-arginine-α-ketoglutarate (2:1) reaction solution.

[0033] (3) Sterilization by ceramic membrane filtration

[0034] After the reaction, the reaction liquid passes through the ceramic membrane first, and the retentate is dialyzed with 4m3 pure ...

Embodiment 2

[0044] A method for preparing L-arginine-alpha-ketoglutarate by a biological enzymatic method, comprising the following steps:

[0045] (1) Preparation of reaction solution

[0046] Add 25m³ of pure water to the 50m³ reactor, then add 3000kg of glutamic acid and 3640kg of arginine, adjust the pH of the solution to 6.9, and dilute to 30m³ with pure water.

[0047] (2) Enzyme reaction

[0048] Add 5U / mL glutamate oxidase and 250U / mL catalase to the reaction solution to start the reaction, control the dissolved oxygen to 20%, control the temperature to 39.5±0.5°C, control the pH to 6.9, and use a biosensor to detect after 8 hours of reaction Without glutamic acid, add 3462kg of arginine to obtain L-arginine-α-ketoglutarate (2:1) reaction solution.

[0049] (3) Sterilization by ceramic membrane filtration

[0050] After the reaction, the reaction liquid passes through the ceramic membrane first, and the retentate is dialyzed with 6m3 pure water; the retentate after dialysis is ...

Embodiment 3

[0060] A method for preparing L-arginine-alpha-ketoglutarate by a biological enzymatic method, comprising the following steps:

[0061] (1) Preparation of reaction solution

[0062] Add 25m³ of pure water to the 50m³ reactor, then add 3000kg of glutamic acid and 3658kg of arginine, adjust the pH of the solution to 7.0, and dilute to 30m³ with pure water.

[0063] (2) Enzyme reaction

[0064] Add 5U / mL glutamic acid oxidase and 400U / mL catalase to the reaction solution to start the reaction, control the dissolved oxygen to 25%, control the temperature to 38.5±0.5°C, add arginine to control the pH 7.0, and react for 10 hours Afterwards, a biosensor was used to detect the absence of glutamic acid, and 3444 kg of arginine was added to obtain a L-arginine-α-ketoglutarate (2:1) reaction solution.

[0065] (3) Sterilization by ceramic membrane filtration

[0066] After the reaction, the reaction liquid passes through the ceramic membrane first, and the retentate is dialyzed with 4m3...

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Abstract

The invention discloses a method for preparing L-arginine-alpha-oxoglutarate through a bio-enzyme method. The method comprises the following steps: generating alpha-oxoglutarate by catalyzing glutamicacid through D-glutamic oxidase, adding arginine, preparing L-arginine-alpha-oxoglutarate (2 to 1), and performing extraction refining through ceramic membrane sterilization, ultrafiltration membraneprotein purification, active carbon decoloration, vacuum concentration and spray drying. The method disclosed by the invention is simple in process, the produced pure ketone is good in quality, and the zero emission of the waste water is basically realized.

Description

technical field [0001] The invention relates to a method for producing sperm ketone, in particular to a method for preparing L-arginine-α-ketoglutarate by a biological enzyme method. Background technique [0002] Arginine (L-arginine-α-ketoglutarate) not only has the physiological functions of arginine and α-ketoglutarate, but also can be used as a functional nutrition enhancer and liver-protecting drug in clinical applications. It is used to enhance physique, promote rapid muscle growth and trauma recovery, promote the absorption and metabolism of nutrients by liver cells, and enhance energy and physical strength. The physiological mechanism is mainly that testosterone can increase the NO level in the body in a short period of time and enhance protein assimilation. At present, the sales volume of spermone as a functional food in the international market is increasing day by day, and together with bird's ketone, it has received much attention in the fields of medicine, nutri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00
CPCC12P13/00
Inventor 王东阳蔡传康陈正刘朋朋
Owner SHANDONG YANGCHENG BIOLOGY TECH CO LTD
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