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Electrochemical detection method for endocrine disrupter on the basis of enzyme nano-reactor

A nano-reactor and endocrine disrupting technology, applied in the field of analytical chemistry, can solve the problems of undisclosed detection of endocrine disrupting substances, etc., and achieve the effects of being suitable for popularization and application, ensuring catalytic activity, and simplifying operation steps

Inactive Publication Date: 2018-06-29
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The prior art does not disclose a technical solution for the detection of endocrine disruptors based on the electrochemical analysis method for the preparation of enzyme nanoreactors

Method used

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  • Electrochemical detection method for endocrine disrupter on the basis of enzyme nano-reactor
  • Electrochemical detection method for endocrine disrupter on the basis of enzyme nano-reactor
  • Electrochemical detection method for endocrine disrupter on the basis of enzyme nano-reactor

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Embodiment 1

[0032] (1) Use functionalized silica with a particle size of 60nm as a template, and mix it with graphene oxide through hydrophobicity-hydrophobic interaction (the preparation method of silica pellets is the process of hydrolysis and condensation of TEOs: Add 80mL of ethanol, 4.85mL of water, and 3.6mL of ammonia water into the flask, and gradually heat it to a certain temperature under constant stirring, then quickly add the mixed solution of TEOS and ethanol (volume ratio: 0.3875:1), and react for 5 hours to obtain the granules. Diameter is the silica pellet of 60nm);

[0033] The synthesis method of functionalized silica is as follows: add 15M hydrochloric acid, 0.6g polyether F108, and 0.875mL DMDMS to 75mL aqueous solution of silica pellets with a concentration of 1mg / mL to obtain a methylated silica solution, Then neutralize the methylated silicon dioxide solution with ammonia water to prepare the functionalized silicon dioxide.

[0034] Using functionalized silica as a...

Embodiment 2

[0047] (1) Use functionalized silica with a particle size of 250nm as a template, and mix it with graphene oxide through hydrophobic-hydrophobic interaction; the preparation of silica pellets is the process of hydrolysis and condensation of TEOs: take 10mL Add the seed dispersion, 70mL ethanol, 13mL water, and 7.5mL ammonia water into the flask, stir evenly at room temperature, then add the mixture of TEOs and ethanol (volume ratio 1:10) with a constant pressure dropping funnel, and react for 5h. This process is repeated four times to obtain silica pellets with a particle size of 250nm

[0048] The synthesis method of functionalized silica is as follows: add 15M hydrochloric acid, 0.6g polyether F108, and 0.875mL DMDMS to 75mL aqueous solution of silica pellets with a concentration of 1mg / mL to obtain a methylated silica solution , and neutralize the methylated silicon dioxide solution in ammonia water to prepare the functionalized silicon dioxide.

[0049] Using functionaliz...

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Abstract

The invention relates to an electrochemical detection method for an endocrine disrupter on the basis of an enzyme nano-reactor. Through functionalized SiO2 as a template, graphene oxide is mixed therewith via hydrophobic-hydrophobic effect, then the materials are frozen and calcined to prepare graphene foam; then by means of electrostatic adsorption and covalent bond process at the same time, active enzyme is immobilized in pores in the graphene foam in order to establish a CYP3A4 / PNGF enzyme reactor. The invention discloses the electrochemical analysis method which is low in cost, has simpleoperations and is convenient and quick. The enzyme nano-reactor allows pore diameter to be adjusted, so that through amperometry, metabolic behavior of the endocrine disrupter can be quickly and accurately detected. The method has high sensitivity and stability and is suitable for promotion and application.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to an electrochemical detection method for endocrine disruptors based on an enzyme nanoreactor. Background technique [0002] Endocrine disrupting chemicals (EDCs) refer to exogenous chemicals that can intervene in the synthesis, secretion, transport, combination, action or decomposition of hormones in organisms, thereby affecting the normal maintenance of organisms and endangering reproduction, development or behavior. substance. EDCs are ubiquitous in pesticides, cosmetics, plastics, food, pharmaceuticals, etc. Common ones include bisphenol A (BPA), phthalates, alkylphenols, and polychlorinated biphenyls (PCBs). Therefore, it is particularly important to establish a low-cost, simple, convenient and quick method for detecting endocrine disruptors. [0003] A large number of studies have shown that CYP450S enzymes may activate some EDCs, making their metabolites hav...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/30G01N27/36
CPCG01N27/308G01N27/3272G01N27/3277G01N27/3278G01N27/36
Inventor 徐璇郑其琴姚玥玮姚成
Owner NANJING UNIV OF TECH
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