Small animal smoke inhalation microenvironment preparation system

A preparation system and microenvironment technology, applied in the field of small animal smoke inhalation microenvironment preparation system, can solve the problems of limited number of experimental animals, expensive equipment, difficult control and monitoring, etc., and achieve accurate controllable and stable smoke concentration High reproducibility and repeatability, avoiding the effect of differences between groups

Inactive Publication Date: 2018-07-03
INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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AI Technical Summary

Problems solved by technology

However, the current model to establish a model of inhalation respiratory system injury disease such as COPD model has two kinds of oronasal exposure system and whole body exposure (that is, the most common fume box or box), and the oronasal exposure system uses a tower chamber structure, the air flow in the exposure chamber is better, and the smoke is more uniform. However, the smoke concentration value of this method cannot be accurately monitored, the number of parallel experimental animals is limited, and the equipment cost is relatively expensive; The ventilation and gas quality of the box are poor, and it is not easy to control and monitor precise parameters. There are deficiencies such as poor repeatability and lack of standardized procedures and measurements. Can be self-made, so it is the most common in domestic laboratories

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Effect test

Embodiment 1

[0033] Example 1 Preparation of cigarette smoke inhalation microenvironment and its use in the establishment of COPD airway inflammation model

[0034] Smoking or passive smoking is an important factor that endangers human health. Smoking causes respiratory and cardiovascular and cerebrovascular diseases is also a hot spot in modern medical research. The stable establishment of small animal disease models caused by cigarette inhalation is the basic condition for the research on the pathogenic mechanism of related diseases and the development of therapeutic drugs. Based on this, the small and medium-sized animal smoke inhalation microenvironment preparation system of the present invention can be used for the establishment of small animal (mice, rats, and guinea pigs) cigarette smoke inhalation-induced respiratory system, cardiovascular and cerebrovascular systems and other disease models. The system has stable smoke distribution The characteristics of uniformity, accurate and cont...

Embodiment 2

[0043] Example 2 Preparation of aerosolized inhalation microenvironment for antigens such as OVA and its application in establishment of asthma airway inflammation model

[0044] Asthma is currently one of the most common respiratory diseases in clinical practice. It has seriously threatened human health and is affected by environmental factors. Its incidence is still increasing year by year. It is estimated that by 2025, there will be as many as 400 million asthma patients worldwide. Inhalation of antigens is an important position in the occurrence and development of asthma. The inhalation of antigens in the environment stimulates the body to produce a large amount of IgE, and these antigen-specific IgE bind to the FcεRI on the surface of mast cells and basophils to activate mast cells , Causing degranulation reaction and release of biologically active inflammatory mediators. Therefore, antigen inhalation is an important method to establish an animal asthma model, and nebulized ...

Embodiment 3

[0052] Example 3 Preparation of PM2.5 inhalation microenvironment and establishment of a model of body damage induced by PM2.5

[0053] Epidemiological studies have found that the increase in the concentration of atmospheric particulate pollutants is associated with the occurrence and development of multiple systems such as breathing, cardiovascular and cerebrovascular and immune systems, as well as respiratory system inflammation, asthma, COPD, atherosclerosis, stroke, and diabetes. Closely related, as many as 3.2 million people worldwide die from atmospheric particulate pollution each year. Among them, PM2.5 has become one of the main factors threatening human health. However, so far, there is still a lack of effective methods for the establishment of human health damage disease models caused by atmospheric particulate matter (especially PM2.5), which greatly affects the research on the mechanism of PM2.5-induced body damage and its prevention. Based on this, the small and medi...

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Abstract

The invention discloses a small animal smoke inhalation microenvironment preparation system, which belongs to the technical field of biological and environmental medicine research and scientific research instruments, and is used for simulating a respiratory inhalation microenvironment, and preparing small animal respiratory system disease models including a chronic obstructive pulmonary disease model, an asthma model, a bronchial inflammation model, a PM2.5 inhalation injury model and a model antigen or drug inhalation injury model. The small animal smoke inhalation microenvironment preparation system is characterized by comprising a microenvironment box body, a main control box, a model preparation device, and an air exhaust device. During operation, smoke or air containing PM2.5 or containing antigen (or drug) generated in the model preparation device is injected into the microenvironment box body by means of an injection pump, real-time monitoring is realized by means of a measurement and control circuit, and a microenvironment in the microenvironment box body is fed back and regulated in real time according to a set limit value. The inhalation microenvironment prepared by the small animal smoke inhalation microenvironment preparation system has the advantages of precise index control, high stability, high repeatability and the like, is multi-purpose, and effectively saves the experimental costs of the instrument and equipment.

Description

Technical field [0001] The patent of the invention relates to a small animal smoke inhalation microenvironment preparation system, which is used to simulate the breathing and inhalation microenvironment of small animals to prepare small animal chronic obstructive pulmonary disease (COPD), asthma, bronchial inflammation, PM2.5 inhalation airway damage , Model drug or antigen atomized inhalation triggers a respiratory system disease model; and can be used to study the efficacy of therapeutic drug atomized inhalation, belonging to the technical field of biological and medical scientific research instruments. Background technique [0002] Respiratory diseases (mainly including asthma and COPD) have become one of the top ten causes of death in China. Among them, as far as COPD is concerned, the number of deaths due to it is as high as 1.28 million every year. Moreover, affected by factors such as increasingly serious atmospheric pollution (especially PM2.5), smoking and aging populati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01M99/00A61D7/04
CPCA61D7/04G01M99/008
Inventor 林明宝侯琦马培白金叶董鹏
Owner INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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