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A kit for detecting serum specific protein in yin-deficiency lit constitution and its application

A specific protein and serum albumin technology, which is applied in the field of kits for detecting serum specific proteins in yin deficiency and anger constitution, can solve the problem of lack of quantitative diagnostic indicators, and achieve scientific, high sensitivity and specificity. Effect

Active Publication Date: 2020-06-05
李继承
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main method of clinically judging yin deficiency and getting angry is through interrogation and observation of tongue and pulse conditions, and there is a lack of quantitative diagnostic indicators

Method used

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  • A kit for detecting serum specific protein in yin-deficiency lit constitution and its application
  • A kit for detecting serum specific protein in yin-deficiency lit constitution and its application
  • A kit for detecting serum specific protein in yin-deficiency lit constitution and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Screening and Verification of Serum Specific Proteins in Yin-deficiency and Excessive Fire Constitution

[0045] According to iTRAQ-2DLC-MS / MS technology, it was found that compared with the peaceful constitution, the concentration of complement protein C9 and serum albumin in the serum of the yin-deficiency and upper-fire constitution decreased significantly, that is, the expression levels of complement protein C9 and serum albumin were significantly down-regulated . Therefore, verification is performed by this example.

[0046] (1) Sample collection: According to the Chinese medicine industry standard of the People's Republic of China "Diagnostic and Curative Criteria of Traditional Chinese Medicine Syndrome Diagnosis and Efficacy Standards" of TCM disease diagnostic criteria and syndrome classification diagnostic criteria for aphtha, and according to the main syndrome and secondary syndrome of yin deficiency, the samples of yin deficiency were collected. 4...

Embodiment 2

[0060] Example 2 The composition of the kit for detecting the serum specific protein in Yin-deficiency and getting angry constitution

[0061]In this example, the kit for detecting serum-specific proteins in yin-deficiency and upper-fire constitution includes a microtiter plate coated with an anti-complement protein C9 antibody, a microtiter plate coated with an anti-serum albumin antibody, and a protein standard for complement protein C9 Protein standards for serum albumin, biotinylated antibody for complement protein C9, biotinylated antibody for serum albumin, horseradish peroxidase-labeled avidin, diluent, washing solution, substrate solution, and stop liquid.

[0062] In this embodiment, the enzyme plate coated with anti-complement protein C9 antibody was purchased from Abcam Company, and its model was ab137912; the enzyme plate coated with anti-serum albumin antibody was purchased from Raybiotech Company, and its model was ELH-Albumin -1; the protein standard product of...

Embodiment 3

[0064] Example 3 Establishment of the Logistic Stepwise Regression Model of the Kit for Detecting Serum-Specific Proteins in Yin-deficiency and Excessive Fire Constitution

[0065] The kits for detecting serum specific proteins in Yin-deficiency and upper-fire constitution were used to detect the concentrations of complement protein C9 and serum albumin in the serum of people with Yin-deficiency and upper-fire constitution, and in the serum of people with mild constitution.

[0066] (1) Sample collection

[0067] According to the TCM disease diagnostic criteria and syndrome classification diagnostic criteria for aphtha in the Chinese medicine industry standard of the People's Republic of China "Standards for Diagnosis and Curative Effects of TCM Diseases and Syndrome", according to the main and secondary syndromes of yin deficiency, 40 serum samples were collected. , Serum samples of 40 cases of normal quality, excluding AIDS, tuberculosis, hepatitis, diabetes, asthma, chronic...

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Abstract

The invention discloses a kit for detecting serum specific protein in constitution with hyperactivity of fire due to yin deficiency and application thereof, and belongs to the field of serum biological markers. The kit includes an ELISA plate coated with an anti-complement protein C9 antibody, an ELISA plate coated with an anti-serum albumin antibody, a protein standard of complement protein C9, aprotein standard of serum albumin, a biotin-labeled antibody of the complement protein C9 and a biotin-labeled antibody of the serum albumin. The kit uses the complement protein C9 and the serum albumin as diagnostic markers, and determines the constitution with hyperactivity of fire due to yin deficiency by measuring the concentration of the complement protein C9 and the serum albumin, traditional Chinese medicine syndrome diagnosis and systems biology are organically combined, an experimental basis is provided for explaining the hyperactivity of fire due to yin deficiency in traditional Chinese medicine in modern biology, and a biological basis of the hyperactivity of fire due to yin deficiency is reflected; the diagnosis sensitivity of the kit is 81.2%, the specificity is 92.3%, and the kit has the advantages of high accuracy and easy operation.

Description

technical field [0001] The invention relates to the field of serum biological markers, in particular to a kit for detecting serum specific proteins in yin-deficiency and upper-fire constitution and its application. Background technique [0002] Inflammation due to yin deficiency is a common sub-health state, very common in the population, prone to aphthous ulcers, rapid onset, manifested as burning pain in the mouth, dry mouth and fatigue, round or oval ulcers in the oral mucosa, mild ulcers around the mouth Hyperemia, red tongue with little moss, thready and stringy pulse, often recurs after healing. At present, the main method of clinically judging yin deficiency and internal heat is through interrogation and observation of tongue and pulse conditions, and there is a lack of quantitative diagnostic indicators. [0003] The characteristics of systems biology are integrity and timeliness, which is relatively consistent with the overall concept of traditional Chinese medicin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/535
CPCG01N33/535G01N33/6893G01N2333/4716
Inventor 李继承陈静
Owner 李继承
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