Applications of selenium acidified atractylodes macrocephala polysaccharides in preparation of drugs or health care products
An Atractylodes macrocephala polysaccharide and selenium acidification technology is applied in the field of biomedicine to achieve the effects of improving antioxidant capacity and immunity, and improving animal anti-stress capacity and body immunity.
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Embodiment 1
[0021] Embodiment 1, the preparation of selenated Atractylodes macrocephala polysaccharide
[0022] Put 1.00g Atractylodes polysaccharide powder into a three-necked flask, add HNO with a volume fraction of 0.3% to 0.8% 3 100mL aqueous solution, heated and stirred to dissolve all the polysaccharides, when the temperature of the water bath was raised to 60-80°C, add 0.8g Na 2 SeO 3 and 1.44g BaCl 2 , Reaction 6~14h. After the reaction was finished, the reaction solution was cooled to room temperature and washed with anhydrous Na 2 CO 3 Adjust the pH of the reaction solution to 5-6, then add an appropriate amount of anhydrous Na 2 SO 4 Precipitation of unreacted BaCl 2 , centrifuged to remove the precipitate, dialyzed, take a small amount of dialysate and add ascorbic acid for detection, stop dialyzing when there is no red color, and freeze-dry to obtain Atractylodes atractylodes selenide polysaccharide. Taking the selenium content of this selenized polysaccharide as an i...
Embodiment 2
[0041] Embodiment 2, in vivo antioxidant activity of selenated Atractylodes macrocephala polysaccharide
[0042]In this example, a completely randomized block design was adopted, and 60 healthy weaned mice of similar quality were randomly divided into 5 treatment groups, which were normal saline group, cyclophosphamide group, Atractylodes polysaccharide group, Atractylodes selenium polysaccharide group, subgroup Sodium selenate group. Twelve mice in each group were administered continuously for 14 days. On the second day of intragastric administration, except for the blank group, the mice in the other groups were injected with 50 mg / kg cyclophosphamide intraperitoneally for 5 consecutive days to prepare the immunocompromised mouse model. The normal control group and the model group were given equal volumes of normal saline, the selenium polysaccharide group was intragastrically administered 0.1 mg / kg (calculated as selenium), the dose of the sodium selenite group was 0.1 mg / kg...
Embodiment 3
[0064] Example 3, Pharmacodynamic Study of Selenated Atractylodes Rhizoma Polysaccharides
[0065] (1) Effects of selenated Atractylodes polysaccharides on the immune organs and carbon clearance function of mice
[0066] Preparation of Indian ink: Take 1 bottle of Indian ink, shake it repeatedly until it is uniform, and dilute it 4 times with normal saline for use.
[0067] Grouping and administration method: This experiment adopted a completely randomized block design, and 60 healthy weaned mice of similar weight were randomly divided into 5 treatment groups, namely normal saline group, cyclophosphamide group, Atractylodes polysaccharide group, Atractylodes polysaccharide group, and Atractylodes polysaccharide group. Selenium polysaccharide group, sodium selenite group. Twelve mice in each group were administered continuously for 14 days. On the second day of intragastric administration, except for the blank group, the mice in the other groups were injected with 50 mg / kg cyc...
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