Repair preparation for placenta subtotal stem cell and mesenchymal stem cell mixed culture extract and preparation method thereof

A technology of subpluripotent stem cells and stem cells, applied in the field of cell culture, can solve the problems of low content of active ingredients in the culture solution, increased skin irritation, poor cosmetic effect, etc., and achieve excellent repair function, excellent whitening, and quality. Balanced effects

Inactive Publication Date: 2018-07-13
北京臻溪谷医学研究中心(有限合伙)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to promote cell proliferation, most of the medium that secretes a large amount of biologically active substances is serum medium, and some components in it have a high risk of use; while the serum-free medium with relatively simple components is not conducive to the growth of stem cells and the release of biologically active substances , leading to the extremely low content of active ingredients in the obtained culture solution, poor cosmetic effect, and obvious side effects caused by large usage
[0003] On the other hand, most of the stem cell culture medium preparations currently used in the beauty industry are freeze-dried preparations. Due to the complexity of the composition of the stem cell culture medium, the freeze-drying process is likely to cause qualitative changes in some components, and a large number of insoluble substances are produced during the reconstitution process. substances, and the irritation to the skin increases, and the safety decreases

Method used

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  • Repair preparation for placenta subtotal stem cell and mesenchymal stem cell mixed culture extract and preparation method thereof
  • Repair preparation for placenta subtotal stem cell and mesenchymal stem cell mixed culture extract and preparation method thereof
  • Repair preparation for placenta subtotal stem cell and mesenchymal stem cell mixed culture extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]A preparation for repairing placental sub-totipotent stem cells and mesenchymal stem cell mixed culture extract, including the following components by weight: placental sub-totipotent stem cell and mesenchymal stem cell mixed culture extract 50 and filler 20; The preparation method of the mixed culture extract of totipotent stem cells and mesenchymal stem cells is as follows:

[0040] 1) Aseptically collect placental tissue, and obtain placental subtotipotent stem cells through washing, digestion and separation steps;

[0041] 2) Aseptically collect placental tissue, and obtain mesenchymal stem cells through washing, digestion and separation steps;

[0042] 3) Placental subtotipotent stem cells were divided into 2×10 4 cells / ml density, mesenchymal stem cells according to 8×10 4 cells / ml, co-inoculate in the primary serum-free culture medium, change the medium every 3 days, culture for 7 days, harvest the primary mixed cells, and collect and combine the cultivated cult...

Embodiment 2

[0048] A repairing preparation of placental subtotipotent stem cells and mesenchymal stem cell mixed culture extract, including the following components by weight: placental subtotipotent stem cell and mesenchymal stem cell mixed culture extract 70 and filler 30; The preparation method of the mixed culture extract of totipotent stem cells and mesenchymal stem cells is as follows:

[0049] 1) Aseptically collect placental tissue, and obtain placental subtotipotent stem cells through washing, digestion and separation steps;

[0050] 2) Aseptically collect placental tissue, and obtain mesenchymal stem cells through washing, digestion and separation steps;

[0051] 3) Placental subtotipotent stem cells were divided into 2×10 4 cells / ml density, mesenchymal stem cells according to 8×10 4 cell / ml density, mixed with the culture medium to obtain a mixed cell suspension, and inoculated in the serum-free culture medium; at 37°C, 5% CO 2 Cultivate in humid air, replace all the cultur...

Embodiment 3

[0057] A repair preparation of placental subtotipotent stem cells and mesenchymal stem cell mixed culture extracts, the difference from Example 2 is that the primary serum-free culture medium is based on OPTI-MEM culture medium, and also includes the following concentrations of Ingredients: 15ug / mL D-glucose, 13ug / mL soybean lecithin and 8ug / mL phenylalanine.

[0058] The passage serum-free culture fluid is based on the OPTI-MEM culture fluid, and also includes components at the following concentrations: 10ug / mL diaminoethane polyacrylamide, 13ug / mL soybean lecithin, 15ug / mL lecithin and 8ug / mL Phenylalanine.

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Abstract

The invention relates to a repair preparation for a placenta subtotal stem cell and mesenchymal stem cell mixed culture extract and a preparation method thereof. The preparation method comprises the following steps: performing mixed culture on placenta subtotal stem cells and mesenchymal stem cells in a serum-free culture medium to obtain a primary culture solution medium and a subculture solutionand mixing for further treatment to obtain the placenta subtotal stem cell and mesenchymal stem cell mixed culture extract. The repair preparation can be prepared into a freeze-dried preparation, issafe in composition, low in irritation to skin, high in active component content, capable of generating excellent whitening, anti-aging and repairing functions only through smaller dosage and strong in traumatic skin injury repair function, has better functions to the skin and meanwhile has better treatment effect on acne.

Description

technical field [0001] The invention belongs to the field of cell culture, and in particular relates to a repair preparation of placental subtotipotent stem cells and mesenchymal stem cell mixed culture extracts and a preparation method thereof. Background technique [0002] Stem cell culture fluid contains a large amount of biologically active substances. In the prior art, stem cell culture fluid is used in the beauty industry, and cosmetics containing stem cell culture fluid are used for a long time, which has functions such as whitening, wrinkle removal, complexion improvement, and ultraviolet damage repair. However, in order to promote cell proliferation, most of the medium that secretes a large amount of biologically active substances is serum medium, and some components in it have a high risk of use; while the serum-free medium with relatively simple components is not conducive to the growth of stem cells and the release of biologically active substances , leading to t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K35/50A61P17/02A61P17/10A61K8/99A61Q19/02A61Q19/08C12N5/0735
CPCA61K35/28A61K8/99A61K35/50A61Q19/02A61Q19/08C12N5/0606A61K2300/00
Inventor 刘俊江曹毓琳时兆田王沾白志惠
Owner 北京臻溪谷医学研究中心(有限合伙)
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