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Detoxifying agent of aflatoxins as well as preparation method and application of detoxifying agent

A kind of technology of aflatoxin and detoxification agent, which is applied in the direction of bacteria, application, food science, etc. used in food preparation, can solve the problems such as the influence of nutrient content, incomplete removal, and the removal efficiency is greatly affected by the environment. Achieve the effect of natural raw materials and mild degradation

Active Publication Date: 2018-07-17
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current biological removal methods have problems such as incomplete removal; removal efficiency is greatly affected by the environment; while removing aflatoxin, the content of nutrients is also affected.

Method used

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  • Detoxifying agent of aflatoxins as well as preparation method and application of detoxifying agent
  • Detoxifying agent of aflatoxins as well as preparation method and application of detoxifying agent

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Activation of the strain

[0036] Inoculate the Lactobacillus plantarum strains of the present invention on the activated medium for cultivation, the cultivation temperature is 37°C, and the time is 24h; the activated strains are inoculated in the fermenter, and the temperature is controlled at 37°C to 43°C, and the time is 24h to 36h , to prepare the fermentation broth.

[0037] The activation medium is: MRS medium, the composition is (g / L): peptone 10.0, meat extract 10.0, yeast extract powder 5.0, glucose 20.0, dipotassium hydrogen phosphate 2.0, diammonium hydrogen citrate 2.0, sodium acetate 5.0 , magnesium sulfate 0.58, manganese sulfate 0.28, cysteine ​​0.5, Tween 801.0, adjust pH 6.2-6.4.

[0038] The medium used for fermentation is: MRS medium, the composition of which is the same as above.

[0039] After the fermentation is completed, the bacterial content in the bacterial liquid is ≥10 8 cfu / mL.

[0040] The said Lactobacillus plantarum numbered BNCC 3364...

Embodiment 2

[0047] A detoxification agent for aflatoxin, which is prepared by asynchronously fermented plant components and laccase in a mass ratio of 97%:3%;

[0048] The specific preparation method is:

[0049] (1) Crush 10g of tangerine peel, 10g of litsea cubeba, 10g of scutellaria baicalensis, and 6g of myrtle to 40 mesh, add 8 times the weight of ultrapure water, stir well, steam sterilize at 100°C for 15min, and cool to room temperature;

[0050] (2) According to 10% inoculum amount, add Bacillus subtilis bacterium liquid to the material after cooling, 200rpm, 33 ℃, aerobic fermentation until the number of Bacillus subtilis ≥ 10 8 cfu / mL;

[0051] (3) Adding 1.5% sterile glucose by weight to the material after aerobic fermentation, adding Lactobacillus plantarum by 10% inoculation amount, and anaerobically fermenting until the number of Lactobacillus plantarum ≥ 10 8 cfu / mL;

[0052] (4) Add 3% laccase by weight to the above fermented liquid and mix evenly.

Embodiment 3

[0054] A detoxification agent for aflatoxin, which is prepared by asynchronously fermented plant components and laccase in a mass ratio of 98%: 2%;

[0055] The specific preparation method is:

[0056] (1) Grind 20g of tangerine peel, 20g of litsea cubeba, 15g of scutellaria baicalensis, and 8g of myrtle to 40 mesh, add 8 times the weight of ultrapure water, stir well, steam sterilize at 100°C for 15min, and cool to room temperature;

[0057] (2) According to 10% inoculum amount, add Bacillus subtilis bacterium liquid to the material after cooling, 200rpm, 33 ℃, aerobic fermentation until the number of Bacillus subtilis ≥ 10 8 cfu / mL;

[0058] (3) Adding 1.5% sterile glucose by weight to the material after aerobic fermentation, adding Lactobacillus plantarum by 10% inoculation amount, and anaerobically fermenting until the number of Lactobacillus plantarum ≥ 10 8 cfu / mL;

[0059](4) Add 2% laccase by weight to the above fermented liquid and mix evenly.

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Abstract

The invention discloses a detoxifying agent of aflatoxins, as well as a preparation method and application of the detoxifying agent, and belongs to the technical field of removal of aflatoxins. The detoxifying agent of aflatoxins is prepared from plant components and laccase after asynchronous fermentation in the mass ratio of the plant components to the laccase being (97-99%) to (3-1%). The plantcomprises the following components of 10-30g of citrus reticulata peel, 10-30g of fructus litsease, 10-20g of radix scutellariae and 6-10g of rhodomyrtus tomentosa. The detoxifying agent of aflatoxins is natural in raw materials, green and safe, and warm in degrading effect on the aflatoxins, and can effectively degrade aflatoxins, and the degrading rate is as high as 95% or above.

Description

technical field [0001] The invention belongs to the technical field of aflatoxin removal, and in particular relates to a detoxification agent for aflatoxin, its preparation method and application. Background technique [0002] Aflatoxins are the most toxic and carcinogenic natural pollutants found today. It not only causes great harm to human and animal health, but also brings huge economic losses. At present, the research on the detoxification method of aflatoxin has become a hot spot for scientific researchers in various countries. Compared with physical and chemical methods, biological detoxification method has unique advantages such as high safety factor, strong specificity, and green cleaning, and is regarded as the most promising and potential detoxification method. The current biological removal methods have problems such as incomplete removal; removal efficiency is greatly affected by the environment; while removing aflatoxin, the content of nutrients is also affec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/20
CPCA23L5/27A23L5/28A23V2400/169
Inventor 杨庆利孔奕凡于春娣唐娟赵海燕
Owner QINGDAO AGRI UNIV
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