A kind of aflatoxin detoxification agent, its preparation method and application

A technology for aflatoxin and detoxification agent, which is applied in the directions of bacteria, application, and fermentation used in food preparation, can solve the problems of the influence of nutrient content, incomplete removal, and the removal efficiency is greatly affected by the environment. Natural raw materials, mild degradation effect

Active Publication Date: 2021-07-20
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current biological removal methods have problems such as incomplete removal; removal efficiency is greatly affected by the environment; while removing aflatoxin, the content of nutrients is also affected.

Method used

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  • A kind of aflatoxin detoxification agent, its preparation method and application
  • A kind of aflatoxin detoxification agent, its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Activation of strains

[0036] The plant lactobacterial species of the present invention is inoculated in the activation medium for culture, and the culture temperature is 37 ° C, time 24 h; inoculate activated strain in the fermenter, control temperature 37 ° C ~ 43 ° C, time 24h ~ 36h Preparation of fermentation liquid.

[0037] The activation medium is: MRS medium, component (g / L): protein 10.0, meat 10.0, yeast powder 5.0, glucose 20.0, potassium hydrogen phosphate 2.0, sodium hydrogen hydroxide 2.0, sodium acetate 5.0 , Magnesium sulfate 0.58, manganese sulfate 0.28, cysteine ​​0.5, Tween 801.0, pH 6.2-6.4.

[0038] The medium used in fermentation is: MRS medium, the ingredient is the same.

[0039] After the fermentation is completed, the bacterial content of the bacterial liquid is ≥10 8 CFU / ml.

[0040] The plant Lactobacillus No. BNCC 336469 was purchased from Beijing North Naburo Biological Technology Research Institute.

[0041] The strain strain was seeded i...

Embodiment 2

[0047] An detoxification agent for xulantoxin is prepared from the mass of the plant component after asynchronous fermentation and the lacca of 97% by: 3%;

[0048] The specific preparation method is:

[0049] (1) Put the tangerine 10g, 10g of the mountain, the mountain, and 6g, the Tajin Niang 6g, smash to 40 mes, add 8 times the weight ultrapure water, stir even, 100 ° C steam sterilization for 15 min, cooled to room temperature;

[0050] (2) Press 10% inoculation, add Bacillus aerugini, 200 rpm, 33 ° C, 200 rpm, 33 ° C, and aerobic fermented bacteria to the subtilum of Bacillus bracicobacterium 8 CFU / ml;

[0051] (3) Add a weight of 1.5% in the material after oxygen fermentation, inocation, inoculate 10%, add plant Lactobacillus, anaerobic fermentation to the number of plant Lactobacillus number ≥10 8 CFU / ml;

[0052] (4) The number of parts by weight of the weight fraction is 3% paint in the fermentation broth, and the mix is ​​uniform.

Embodiment 3

[0054] An detoxification agent for xylus toxin is prepared by the plant component after asynchronous fermentation and lacca of 98%: 2%;

[0055] The specific preparation method is:

[0056] (1) Put the tangerine peel 20g, mountain sand 20g, astragalus, Taojin Niang 8g, crush to 40 mesh, add 8 times the weight ultrapure water, stir well, steam sterilization at 100 ° C for 15 min, cool to room temperature;

[0057] (2) Press 10% inoculation, add Bacillus aerugini, 200 rpm, 33 ° C, 200 rpm, 33 ° C, and aerobic fermented bacteria to the subtilum of Bacillus bracicobacterium 8 CFU / ml;

[0058] (3) Add a weight of 1.5% in the material after oxygen fermentation, inocation, inoculate 10%, add plant Lactobacillus, anaerobic fermentation to the number of plant Lactobacillus number ≥10 8 CFU / ml;

[0059](4) Add a weight of the weight of the weight of the fermentation liquid to be 2% laccase, and mix evenly.

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Abstract

The invention discloses a detoxification agent for aflatoxin, its preparation method and application, and belongs to the technical field of aflatoxin removal. The aflatoxin detoxification agent of the present invention is prepared by asynchronously fermented plant components and laccase in a mass ratio of 97-99%: 3-1%; 30g, Litsea cubeba 10-30g, Scutellaria baicalensis 10-20g, Myrtle 6-10g. The raw material of the aflatoxin detoxification agent of the present invention is natural, green and safe, has a mild degradation effect on the aflatoxin, can effectively degrade the aflatoxin, and has a degradation rate as high as 95%.

Description

Technical field [0001] The present invention belongs to the field of yellowlaxin removal technique, and in particular, the present invention relates to a detrographic agent of xulantoxin, and the preparation method and application thereof are used. Background technique [0002] The yellowlaxin is the toxicity and the strongest natural pollutant discovered today. It is not only a great harm to the health of humans and animals, but also brings huge economic losses. At present, the diaphragm of xylushin toxin has become a hot spot for research workers in various countries. Biological detoxification method has a unique advantage of high safety coefficients, high-profile, green cleaning, etc., which is considered to be the most developed and potential. The current biological removal method is not complete; the removal efficiency is greatly affected by the environment; while removing the xilexin toxin, the nutrient content is also affected. Inventive content [0003] For the problems ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/04A23L5/20
CPCA23L5/27A23L5/28A23V2400/169
Inventor 杨庆利孔奕凡于春娣唐娟赵海燕
Owner QINGDAO AGRI UNIV
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