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Method for preparing algae-selenium polysaccharide from enteromorpha prolifera

The technology of seaweed selenium polysaccharide and prolifera polysaccharide, which is applied in the field of prolifera seaweed selenium polysaccharide, can solve the problems of inability to achieve effective extraction of polysaccharide, unable to meet the requirements of efficiency, low extraction efficiency of prolifera polysaccharide, etc., and to improve bioavailability , rate increase, molecular fragment reduction effect

Active Publication Date: 2018-07-20
青岛鑫康达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The extraction time of hot water extraction-ultrasonic method and hot water extraction-microwave-assisted method is short, but the yield of polysaccharides is the lowest, and the yield of polysaccharides in simple hot water extraction method is also low. Therefore, hot water extraction method , hot water extraction-ultrasonic method and hot water extraction-microwave-assisted method cannot meet the requirements for efficiency in production
Compared with the first three extraction methods, the cellulase extraction method and hot water extraction-papain combined extraction method have greatly improved the polysaccharide yield, but compared with the increase in extraction time, the extraction efficiency is very low. low, and the extraction process of the above five enteromorpha polysaccharides does not involve desalination or deproteinization steps, the purity of the obtained polysaccharides is low
[0005] Moreover, in the prior art, the pretreatment of Enteromorpha still stays on a single pulverization, because the particle size after pulverization is relatively large, the effective extraction of polysaccharides cannot be realized, and the extraction efficiency of Enteromorpha polysaccharides in the whole production process is low, which cannot be obtained. Meet the needs of mass production

Method used

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  • Method for preparing algae-selenium polysaccharide from enteromorpha prolifera

Examples

Experimental program
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Effect test

Embodiment 1

[0035] (1) Pulverization of Enteromorpha: adopt ball milling method combined with nano crushing treatment method to carry out efficient combined pretreatment of Enteromorpha, specifically

[0036] ① Coarse crushing: coarsely crush the dry Enteromorpha with a pulverizer, pass the crushed material through a 40-60 mesh sieve, and take the sieved material;

[0037] ②Ball milling treatment: Add the sieved material into the ball mill tank, the mass ratio of Enteromorpha to balls is 1:5, the speed of the ball mill is 700rpm, and the ball mill is 5min;

[0038] ③Nano-crushing: take the Enteromorpha powder treated in step ②, add the deionized water of 1 times the quality of the Enteromorpha powder, homogenize, pressurize to 1000kg / cm with a high-pressure pump 2 , enter the oscillation channel of the ultrasonic collider with a high-speed flow of 200m / sec, and achieve nano-scale particle crushing and dispersion of the homogeneous liquid at a working frequency of 0.2MHZ;

[0039] (2) Ext...

Embodiment 2

[0048] The difference from Example 1 is that prior to the ball milling process in step (1), the pulverized Enteromorpha is subjected to freezing and shaking pretreatment; It was preserved for 3 hours, and the treatment was repeated 3 times, and other parameters were the same, and the solid enteromorpha selenium polysaccharide was obtained.

Embodiment 3

[0050] The difference from Example 1 is that only step (1) is carried out: the dry Enteromorpha is coarsely crushed with a pulverizer, the pulverized matter is passed through a 40-60 mesh sieve, and the under-sieve is taken, and other parameters are the same to obtain solid Enteromorpha seaweed selenium polysaccharides.

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Abstract

The invention provides a method for preparing algae-selenium polysaccharide from enteromorpha prolifera. According to the method, a traditional technology is improved, reaction is fast, selenium content of algae-selenium polysaccharide prepared from the enteromorpha prolifera is 20-120 mg / g, molecular weight is low, and biological activity high; the enteromorpha prolifera as a raw material of algae-selenium polysaccharide prepared with the method is widely sourced and easy to collect, and the enteromorpha prolifera harmful to seawater is turned into wealth, so that the environment is protected.

Description

technical field [0001] The invention relates to a preparation method of enteromorpha seaweed selenium polysaccharide, in particular to a method for obtaining enteromorpha seaweed selenium polysaccharide by using enteromorpha as raw material through a series of treatments. Background technique [0002] Enteromorpha, grass-green algae, tubular membranous, clustered, with obvious main branches, slender branches, up to 1 meter high. The base is attached to the rock with a fixator, and grows on tidal flats and gravels in the mid-tidal zone. Due to global climate change, water eutrophication and other reasons, the green tide of the large marine alga Enteromorpha broke out. A large amount of Enteromorpha floats and gathers on the shore, blocking waterways, destroying marine ecosystems, and seriously threatening the development of coastal fisheries and tourism. [0003] Selenium is an essential trace element for humans and animals. It is known as "the fire of life", "the king of a...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 张正兴任媛媛潘惟谦杜吉勇刘国强
Owner 青岛鑫康达生物科技有限公司