A kind of substrate used in electrochemiluminescence analyzer and its preparation method and application

A luminescence analysis and electrochemical technology, applied in the field of electrochemical analysis, can solve the problems of long stability, reduced measurement range, inaccurate detection results of high-concentration clinical samples, etc., to achieve stable performance, ensure specificity and accuracy, formula And the effect of simple and easy preparation method

Active Publication Date: 2021-03-02
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Aiming at the problem that the concentration of tripropylamine in the disclosed formula of the luminescence buffer solution in the above-mentioned prior art is lower than the original concentration, resulting in a decrease in the measurement range and inaccurate detection results of high-concentration clinical samples, the purpose of the present invention is to provide an electrochemiluminescence analyzer The substrate used and its preparation method and application, the measurement range of the substrate of the present invention is the same as that of the original product, the result is accurate and reliable, the preparation is simple, the cost is low, and the stability is long

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  • A kind of substrate used in electrochemiluminescence analyzer and its preparation method and application
  • A kind of substrate used in electrochemiluminescence analyzer and its preparation method and application
  • A kind of substrate used in electrochemiluminescence analyzer and its preparation method and application

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Embodiment

[0054] A substrate used in an electrochemiluminescence analyzer and a preparation method thereof, comprising a luminescence buffer, a measuring cell cleaning solution and a pre-cleaning solution.

[0055] ①The composition and preparation method of luminescence buffer (referred to as Procell) are as follows:

[0056] Composition: 85wt% phosphoric acid 3.2g / L,

[0057] 0.5g / L hydrochloric acid with 2 normal concentration,

[0058] Dichloroacetamide 1.5g / L,

[0059] Isooctyl alcohol polyoxyethylene ether with a degree of polymerization of 14 1.0g / L,

[0060] Lauryl alcohol polyoxyethylene ether 1.2g / L with a degree of polymerization of 9,

[0061] Potassium dihydrogen phosphate 39g / L, and

[0062] Tripropylamine 26g / L.

[0063] Preparation: put the beaker on the balance, set it to zero, then weigh the required components except tripropylamine according to the above formula, add them to the beaker, add 700 ml of water, stir for 5 minutes to dissolve, add tripropylamine, stir ...

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Abstract

The invention discloses a substrate used in an electrochemiluminescence analyzer and its preparation method and application, and relates to the field of electrochemical analysis, including a luminescent buffer solution, a measuring cell cleaning solution and a pre-cleaning solution. The luminescent buffer solution includes a polymerization degree of (12 ‑16) isooctyl alcohol polyoxyethylene ether, and lauryl alcohol polyoxyethylene ether with a degree of polymerization of (6‑10). The measurement range of the substrate of the invention is the same as that of the original product, the result is accurate and reliable, the preparation is simple, the cost is low, and the stability is long.

Description

technical field [0001] The invention relates to the field of electrochemical analysis, in particular to a substrate used in an electrochemiluminescence analyzer and a preparation method and application thereof. Background technique [0002] Electrochemiluminescence immunoassay technology is one of the immunoassay technologies invented by Roche, Switzerland. It is widely used in the quantitative determination of thyroid, sex hormones, bone metabolism, myocardial infarction, tumor markers, infectious disease antigens and antibodies, etc. The reaction principle based on it is the immune response of antigen and antibody. [0003] The reaction process is as follows: ①The instrument absorbs biotin reagents containing antibodies, antigens or enzymes and magnetic microsphere complexes coated with streptavidin, add them to the cuvette for mixing, and react in the incubation plate at 37°C for 9 to 27 minutes. Make the substance to be tested specifically bind to the biotinylated anti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531G01N21/76
CPCG01N21/763G01N33/531
Inventor 田清武岳康孙树凯赵鹏孙异凡吴国才
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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