Silk fibroin micro-needle patch and preparation method thereof

A silk fibroin microneedle and silk fibroin technology are applied in the field of silk fibroin microneedle stickers and their preparation, and can solve the problem of small drug loading, unsuitable for mass production, complex electroplating, and photoetching microchannel casting. Technical preparation and use, etc.

Inactive Publication Date: 2016-08-10
PHARSUN MEDICAL BIOTECHNICS (SHANGHAI) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although traditional metal, glass, silicon and other solid microneedles have good penetration ability to change the skin permeability, the drug is generally applied to the surface of the microneedle, and the drug loading is small, so it is difficult to achieve the therapeutic effect
Traditional metal, glass, silica, silicon and polymer hollow microneedles can improve the defects of low drug loading rate and poor drug release control of solid microneedles by adding drug storage, but it requires complicated electroplating, Complex processes such as photolithography, wet etching, microchannel casting technology, and insert mold preparation and use are high in cost and not suitable for mass production

Method used

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  • Silk fibroin micro-needle patch and preparation method thereof
  • Silk fibroin micro-needle patch and preparation method thereof
  • Silk fibroin micro-needle patch and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Preparation of small molecule silk fibroin solution: the silkworm cocoon shell of 80g is 0.25% Na in 4000mL mass fraction 2 CO 3 In the solution, boil it three times at 98-100°C for 30 minutes each time, and wash it with deionized water. After degumming and drying, silk fibroin was dissolved in 9.3M lithium bromide solution at 60°C with a bath ratio of 1:10, dialyzed in deionized water for 3-4 days, concentrated to 0.09g / mL, and stored in a refrigerator at 4°C for use.

[0030] (2) Preparation of the microneedle layer: The small molecule silk fibroin solution with a concentration of 0.09g / mL was poured into a PDMS (polydimethylsiloxane) mold at a pressure of more than 0.09MPa at 4.5mg per 15*15 array. After vacuum degassing under vacuum conditions, dry at constant temperature and humidity (temperature: 40°C, relative humidity: 75%) for 24 hours;

[0031] (3) Loading of ibuprofen: Dissolve a certain dose of powdered cold medicine ibuprofen in chloroform, pour it on...

Embodiment 2

[0034] (1) Preparation of small molecule silk fibroin solution: the silkworm cocoon shell of 40g is added to 2000mL mass fraction of 0.25% Na 2 CO 3 In the solution, boil it three times at 98-100°C for 30 minutes each time, and wash it with deionized water. After degumming and drying, silk fibroin was dissolved in 9.3M lithium bromide solution at 60°C with a bath ratio of 1:10, dialyzed in deionized water for 3-4 days, concentrated to 0.09g / mL, and stored in a refrigerator at 4°C for use.

[0035] (2) Preparation of the microneedle layer: the small molecule silk fibroin solution with a concentration of 0.09g / mL was poured into a PDMS (polydimethylsiloxane) mold at a density of 0.09g / mL per 15*15 array, and the After vacuum degassing under high temperature conditions, dry at constant temperature and humidity (temperature: 25°C, relative humidity: 65%) for 24 hours;

[0036] (3) Loading of insulin: Dissolve a certain dose of powdered hypoglycemic drug insulin in HCL-Tris buffe...

Embodiment 3

[0039] (1) Preparation of small molecule silk fibroin solution: the silkworm cocoon shells of 800g are added to 40000mL of Na with a mass fraction of 0.25%. 2 CO 3In the solution, boil it three times at 98-100°C for 30 minutes each time, and wash it with deionized water. After degumming and drying, silk fibroin was dissolved in 9.3M lithium bromide solution at 60°C with a bath ratio of 1:10, dialyzed in deionized water for 3-4 days, concentrated to 0.09g / mL, and stored in a refrigerator at 4°C for use.

[0040] (2) Preparation of the microneedle layer: The small molecule silk fibroin solution with a concentration of 0.09g / mL was poured into a PDMS (polydimethylsiloxane) mold at a pressure of more than 0.09MPa at 10.5mg per 15*15 array. After vacuum degassing under vacuum conditions, dry at constant temperature and humidity (temperature: 15°C, relative humidity: 25%) for 24 hours;

[0041] (3) Loading of hyaluronic acid: Dissolve a certain dose of hyaluronic acid, a solid pow...

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Abstract

The invention belongs to the field of medical beauty micro-needles, and in particular relates to a silk fibroin micro-needle patch and a preparation method thereof. The silk fibroin micro-needle patch comprises a backing layer, a micro-needle layer and a pressure-sensitive adhesive coating by which the backing layer and the micro-needle layer are stuck, wherein a plurality of needle-shaped bumps, which are uniformly distributed, are arranged on the micro-needle layer; needle-shaped cavities are formed in the needle-shaped bumps; the volume of the needle-shaped cavities is 40-90% of the volume of the needle-shaped bumps; medicines are kept in the needle-shaped cavities; the micro-needle layer is made from silk fibroin, and the molecular weight of the silk fibroin is lower than 100,000; the silk fibroin micro-needle patch is excellent in mechanical performance under a dry state, the silk fibroin micro-needle patch is sufficient in strength to penetrate into skin, and since the molecular weight of the silk fibroin is lower than 100,000, the micro-needle patch is high in dissolving rate, and as the micro-needle patch penetrates into the skin and gets into contact with body liquid, the micro-needle layer can rapidly dissolve, and subsequently, the medicines can get into contact with the body liquid and can dissolve in and disperse to the inner side of epidermis; therefore, the silk fibroin micro-needle patch is applicable to symptoms which require the rapid release of the medicines and have a relatively high requirement on a blood medicine dose.

Description

technical field [0001] The invention belongs to the field of medical cosmetic microneedles, in particular to a silk fibroin microneedle patch and a preparation method thereof. Background technique [0002] Nowadays, people pay more and more attention to skin care, especially the skin around the eyes. The skin around the eyes is thin and prone to aging and problems. The commonly used eye skin maintenance drugs are mostly macromolecular drugs, which are difficult to penetrate the eye skin and enter the subcutaneous area, so the utilization rate is low. [0003] A microneedle is a micron-sized hollow or solid needle similar to an injection needle. It is made by a micro-fabrication process, with a diameter of 30-80 μm and a length of about several hundred microns. It can just pass through the stratum corneum without touching the pain nerves. It can promote penetration without causing pain and skin damage. It opens a vertical and visible channel on the stratum corneum of the ski...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61M37/00
CPCA61M37/0015A61M2037/0046A61M2037/0053A61M2037/003
Inventor 殷祝平卢神州范语婷方翠翠卢欣旸胥仕林章梦雪
Owner PHARSUN MEDICAL BIOTECHNICS (SHANGHAI) CO LTD
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