An electrospun medical composite fiber drug film capable of loading multiple drugs and its preparation method
A composite fiber and medical technology, which is applied in the field of polyvinyl alcohol/polylactic acid microspheres/nano calcium phosphate medical composite fiber film and its preparation, to achieve the effect of improving repair effect, good application prospect and simple process
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Embodiment 1
[0030] Nano-calcium phosphate was prepared by precipitation method at 37 °C. First prepare the Ca separately 2+ Calcium nitrate aqueous solution and PO with a concentration of 0.0334mol / L 4 3- Diammonium hydrogen phosphate aqueous solution with an ion concentration of 0.02mol / L, then heat and stir the prepared solution on a constant temperature magnetic stirrer at 37°C according to the Ca / P molar ratio of 1.67, and quickly pour the diammonium hydrogen phosphate aqueous solution into In the calcium nitrate aqueous solution, stir vigorously to mix evenly, add concentrated ammonia water dropwise to the solution to adjust the pH to 10-11, react at 37°C for 1 hour, centrifuge the milky white turbid liquid, rinse it with deionized water three times, and obtain a white precipitate of nano-calcium phosphate . Add 500mg of nano-calcium phosphate and 15mg of polyacrylic acid into 50mL of water, and ultrasonically disperse for 3min to obtain nano-calcium phosphate suspension.
[0031...
Embodiment 2
[0035] Adopt the method identical with embodiment 1 to prepare nano-calcium phosphate. Add 100mg of nano-calcium phosphate and 15mg of polyacrylic acid into 50mL of water, and ultrasonically disperse for 3 minutes to obtain a suspension of nano-calcium phosphate.
[0036] Add 50mg of polylactic acid into 5mL of dichloromethane, and continue to sonicate in an ultrasonic cleaner for 10-30min until the polylactic acid is completely dissolved. Immediately inject the obtained suspension into 125mL, 5mg / mL polyvinyl alcohol solution, stir at 1000r / min for 3h, and then ultrasonically disperse for 10min to obtain a suspension of porous polylactic acid microspheres.
[0037] Mix 5mL polylactic acid microsphere suspension and 5mL nano-calcium phosphate suspension evenly, then add 1.4g of polyvinyl alcohol, and stir at 80°C to obtain a composite spinning solution of polyvinyl alcohol, polylactic acid microspheres and nano-calcium phosphate . The composite spinning solution was injected...
Embodiment 3
[0040] Adopt the method identical with embodiment 1 to prepare nano-calcium phosphate. Add 100mg of nano-calcium phosphate and 15mg of polyacrylic acid into 50mL of water, and ultrasonically disperse for 3 minutes to obtain a suspension of nano-calcium phosphate.
[0041] Add 200mg of polylactic acid into 5mL of dichloromethane, and continue to sonicate in an ultrasonic cleaner for 10-30min until the polylactic acid is completely dissolved. Immediately inject the obtained suspension into 100 mL, 5 mg / mL polyvinyl alcohol solution, stir at 1000 r / min for 3 h, and ultrasonically disperse for 10 min to obtain a suspension of porous polylactic acid microspheres.
[0042] Mix 5mL polylactic acid microsphere suspension and 5mL nano-calcium phosphate suspension evenly, then add 0.7g of polyvinyl alcohol, and stir at 80°C to obtain a composite spinning solution of polyvinyl alcohol, polylactic acid microspheres and nano-calcium phosphate . The composite spinning solution was injecte...
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