Solid-phase synthesizing method of polypeptide, synthesized magnetic nanometer probe and application thereof

A solid-phase synthesis and magnetic technology, applied in the preparation method of peptides, magnetic materials, magnetic objects, etc., can solve the problems of difficult to obtain peptides, and achieve the effect of easy separation and extraction

Active Publication Date: 2018-07-24
QINGDAO UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For this reason, the technical problem to be solved by the present invention is to provide a method for solid-phase synthesis of polypeptides to solve the problem that it is difficult to obtain polypeptides with specific sequences or amino acid sequences of specific lengths in the prior art.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Solid-phase synthesizing method of polypeptide, synthesized magnetic nanometer probe and application thereof
  • Solid-phase synthesizing method of polypeptide, synthesized magnetic nanometer probe and application thereof
  • Solid-phase synthesizing method of polypeptide, synthesized magnetic nanometer probe and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 0.1g superparamagnetic Fe 3 O 4 Nanoparticles were dispersed in 100ml of absolute ethanol, added 1.0ml of ammonia, mixed well, then added 200μLTEOS, placed in an ultrasonic machine with a power of 100w, sonicated for 6h, and added 200μLTEOS every 2h for a total of 2 times. After the reaction, The product is magnetically separated and washed with deionized water and absolute ethanol alternately 3 times to obtain Fe 3 O 4 @SiO 2 Nanoparticle

[0044] Take 0.05g of Fe obtained 3 O 4 @SiO 2 Nanoparticles were dispersed in a mixed solution of 30ml absolute ethanol and 30ml deionized water, added 0.075g CTAB, placed in an ultrasonic machine with a power of 100W and sonicated for 2h, added 0.048g resorcinol, 75μLTEOS, stirred for 10min and then added 183μL Formaldehyde solution, 230μL ammonia water, stirred at room temperature for 24h, centrifuged the product, washed with ethanol 3 times to obtain the required Fe 3 O 4 @SiO 2 @PF Three-layer core-shell structured nanoparticles.

[...

Embodiment 2

[0053] 0.1g superparamagnetic Fe 3 O 4 Nanoparticles were dispersed in 100ml of absolute ethanol, added 1.0ml of ammonia, mixed well, then added 200μLTEOS, placed in an ultrasonic machine with a power of 100w, sonicated for 6h, and added 200μLTEOS every 2h for a total of 2 times. After the reaction, The product is magnetically separated and washed with deionized water and absolute ethanol alternately 3 times to obtain Fe 3 O 4 @SiO 2 Nanoparticle

[0054] Take 0.05g of Fe obtained 3 O 4 @SiO 2 Nanoparticles were dispersed in a mixed solution of 30ml absolute ethanol and 30ml deionized water, added 0.075g CTAB, placed in an ultrasonic machine with a power of 100W and sonicated for 2h, added 0.1g resorcinol, 75μLTEOS, stirred for 10min and then added 366μL Formaldehyde solution, 230μL ammonia water, stirred at room temperature for 24h, centrifuged the product, washed with ethanol 3 times to obtain the required Fe 3 O 4 @SiO 2 @PF Three-layer core-shell structured nanoparticles.

[00...

Embodiment 3

[0062] 0.1g superparamagnetic Fe 3 O 4 Nanoparticles are dispersed in 100ml of absolute ethanol, add 1.0ml of ammonia water, mix well, then add 200μL of TEOS, place in a 100w ultrasonic machine, sonicate for 6h, add 200μL of TEOS every 2h, add 2 times, the reaction is over After that, the product was magnetically separated and washed with deionized water and absolute ethanol alternately 3 times to obtain Fe 3 O 4 @SiO 2 Nanoparticle

[0063] Take 0.05g of Fe obtained 3 O 4 @SiO 2 Nanoparticles were dispersed in a mixed solution of 30ml absolute ethanol and 30ml deionized water, added 0.075g CTAB, placed in an ultrasonic machine with a power of 100W and sonicated for 2h, added 0.048g resorcinol, 75μLTEOS, stirred for 10min and then added 183μL Formaldehyde solution, 230μL ammonia water, stirred at room temperature for 24h, centrifuged the product, washed with ethanol 3 times to obtain the required Fe 3 O 4 @SiO 2 @PF Three-layer core-shell structured nanoparticles.

[0064] Take 0.0...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention belongs to the technical field of synthesis of polypeptides, and particularly relates to a solid-phase synthesizing method of a polypeptide with a special sequence, a magnetic nanometerprobe synthesized by the method, and application of the magnetic nanometer probe in screening of tumor-targeted polypeptide medicines. The solid-phase synthesizing method of the polypeptide is characterized in that a magnetic phenolic resin composite nanoparticle is used as a solid-phase carrier to synthesize the polypeptide with special amino acid sequence or amino acid number. The solid-phase synthesizing method of the polypeptide has the advantages that by adopting the magnetic solid-phase carrier, the separation and extraction are convenient; the polypeptide with the special sequence can be simply and conveniently synthesized by the solid-phase carrier with compound structure, and can be used as the magnetic probe for multiple purposes.

Description

Technical field [0001] The invention belongs to the technical field of polypeptide synthesis, and in particular relates to a method for solid-phase synthesis of polypeptides with specific sequences, a magnetic nano probe synthesized by the method, and its application in screening tumor-targeted polypeptide drugs. Background technique [0002] As an organic polymer, peptides have received more and more attention due to their various functions and good biocompatibility. The chemical synthesis of peptides has also become an important area in the field of organic synthesis. At present, the principle of polypeptide synthesis is mainly based on amino acids as the basic unit, and under the action of a catalyst, the amide bond formed by the reaction of the carboxyl group and the amino group between the amino acids will be connected, and thus the growth of the polypeptide chain can be realized. However, in traditional methods of organic synthesis of polypeptides, the connection of carboxy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K1/06C07K1/04B01J13/02H01F1/00A61K49/00
CPCA61K49/00B01J13/02C07K1/042C07K1/047C07K1/06H01F1/0054Y02P20/55
Inventor 丛海林于冰杨振单玉领
Owner QINGDAO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap