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Extraction method and anti-tumor application of linumusitatissimum polyphenol

An extraction method, the technology of flax, which is applied in the field of extracting active ingredients of plants, can solve the problems that the anti-tumor activity of flax polyphenols has not been seen, and achieve the effects of promoting in-depth development and effective utilization, inhibiting cancer cell proliferation, and good anti-cancer activity

Inactive Publication Date: 2018-07-27
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no research on the antitumor activity of flax polyphenols has been seen

Method used

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  • Extraction method and anti-tumor application of linumusitatissimum polyphenol
  • Extraction method and anti-tumor application of linumusitatissimum polyphenol
  • Extraction method and anti-tumor application of linumusitatissimum polyphenol

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of flax polyphenols:

[0030] (1) Get 500g of flax meal and separate it into grain shape;

[0031] (2) Add distilled water at a mass ratio of 1:3, stir in a water bath at 70°C for 1 hour, let stand, filter with double gauze, and rinse;

[0032] (3) Repeat step (2) several times until there is no viscose on the surface of the flax seeds, then completely dry the degummed flax seeds in an oven at 40°C and pulverize to obtain degummed flax powder;

[0033] (4) Get 20g of degummed flax powder, dissolve it in 1600mL of 60% ethanol, and conduct ultrasonic extraction at 50°C for 50 minutes at an ultrasonic power of 400W;

[0034] (5) centrifuge the mixed material liquid obtained in step (4) at 8000×rpm for 5 minutes, discard the precipitate, centrifuge once again under the same conditions, and collect the supernatant;

[0035] (6) Degrease the supernatant obtained in step (5), add ethyl acetate at a volume ratio of 1:1, centrifuge at 11000×rpm for 10 minutes, let ...

Embodiment 2

[0039] Select the DLD1, HCT-116, SW480, SW620, HT-29, A549, HeLa, HepG2, MCF-7 cancer cells and FHC normal cells in the logarithmic growth phase respectively, make the cells into cell suspension, and count the cells every Hole 3×10 3 Inoculated into a 96-well plate at 37°C, 5% CO 2 After culturing in the incubator for 24 hours and the cells adhered to the wall, the old medium was discarded, and medium with different final concentrations of flax polyphenols (0, 0.05, 0.1, 0.3, 0.5, 0.7, 0.9 mg / mL) was added, and six chambers were set up. For parallel wells, continue to culture the culture plate for 24 hours, add 20 μL of 5.0 mg / mL MTT to each well, and incubate for another 4 hours. Remove the culture medium in the plate, add 150 μL of DMSO to each well to dissolve the formazan crystals produced by the living cells, shake on a shaker for 10 min, and measure the absorbance in each well with a microplate reader at a wavelength of 570 nm.

[0040] The flax polyphenols obtained in...

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PUM

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Abstract

The invention provides an extraction method and anti-tumor application of linumusitatissimum polyphenol. The extraction method of the linumusitatissimum polyphenol comprises the following steps of drying linumusitatissimum meal; then, performing crushing; performing degumming treatment and drying for later use; dissolving the degummed linumusitatissimum powder into 60-percent ethanol according tothe mass ratio of 1:(60 to 75); performing 400W power supersonic extraction at 42 to 55 DEG C for 50 to 60min; performing centrifugation treatment on the solution; collecting supernate; adding the materials into ethyl acetate according to the volume ratio of 1:(1 to 1.3) for degreasing; performing multi-time repeated degreasing; performing rotary evaporation concentration on the collected polyphenol solution; performing freeze drying on the rest liquid; collecting dry powder to obtain the linumusitatissimum polyphenol. Extraneous anti-tumor experiments show that the linumusitatissimum polyphenol can obviously inhibit the breeding of various cancer cells without cell toxicity; good anti-tumor activity is realized; the linumusitatissimum polyphenol can be applied to preparation of anti-tumormedicine and health care food.

Description

technical field [0001] The invention relates to the extraction of effective plant components, in particular to the extraction and application of polyphenols in flax herbal plants, specifically to the extraction method of polyphenols in flax, and its application in antitumor drugs and health food. Background technique [0002] Flax (Linumusitatissimum Linn.) is flax for oil, which belongs to the family Linaceae and is an annual herb. The seeds of flax (linseed) are flat and long oval, 4-6mm long, 2-3mm wide, and about 1 mm thick, with an average weight of 3-13mg per seed. The surface is reddish-brown or brown, smooth and shiny, the periphery is gradually thinner, and the middle is slightly convex. One end is blunt and round, and the other end is pointed and slightly oblique. The hilum is located in the depression at the tip. Seed ridge light brown, located on one side edge. The seed coat is thin, and a brown film-like endosperm can be seen after removing the seed coat. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/55A61P35/00A23L33/105A61K131/00
CPCA23L33/105A23V2002/00A61K36/55A61K2236/13A61K2236/15A61K2236/17A61K2236/333A61K2236/51A61K2236/53A61K2236/55A61P35/00A23V2200/30A23V2200/02A23V2250/2132
Inventor 李卓玉杨宇菲张立超剌晓琴李汉卿
Owner SHANXI UNIV
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