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Visual optical sensing method for detection of hydrogen peroxide based on nano-silver Tyndall effect

A hydrogen peroxide, optical sensing technology, applied in the field of nano-sensing, can solve the problems of inappropriate on-site analysis and point-of-care testing applications, lack of portability, and high analysis costs, and achieve low-cost portable qualitative and quantitative detection, specific portable qualitative detection. The effect of quantitative detection and high analytical sensitivity

Inactive Publication Date: 2018-08-10
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limited sensitivity of the naked eye to observe the color change of the solution when the nano-silver material is agglomerated, the detection limit of the target object based on this type of probe is often limited to the micromolar level in optical qualitative analysis or semi-quantitative analysis
In addition, most of the existing nano-silver optical sensing methods need to use expensive and lack of portability UV-Vis spectrophotometer, fluorescence spectrophotometer and other professional analytical instruments for quantitative detection, the analysis cost is high, and it is not suitable for on-site analysis App with instant test

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Detection of hydrogen peroxide based on the Tyndall effect of bare silver nanoparticles.

[0019] The specific implementation process is as follows:

[0020] (1) In a 1.5 mL test tube, mix 900 μL of the hydrogen peroxide sample solution to be tested with 100 μL of a 1000-fold diluted silver nanoparticle solution (with a particle size of about 20 nm, stock solution Made by reducing silver nitrate with sodium borohydride), hydrogen peroxide will oxidize the silver nanoparticles into silver ions.

[0021] (2) Use the red laser beam generated by the laser pointer to irradiate the solution obtained in step (1) to generate a Tyndall effect optical signal, and use a mobile phone to record the gray value of the signal. The concentration of hydrogen peroxide in the sample solution to be tested can be determined according to the following standard curve.

[0022] According to the above steps, the 6 prepared hydrogen peroxide standard solutions (concentrations from 10 -4 to 10 ...

Embodiment 2

[0024] Glucose detection based on the Tyndall effect of bare silver nanoparticles.

[0025] The specific implementation process is as follows:

[0026] (1) In a 1.5 mL test tube, mix 2 μL of the glucose sample solution to be tested with 898 μL of a glucose oxidase solution with a concentration of 10 μg / mL (prepared by sodium dihydrogen phosphate-dipotassium hydrogen phosphate buffer solution with a concentration of 10 mmol / L) , pH 7), and react at room temperature for 3 minutes, glucose oxidase will catalyze the oxidation of glucose to produce hydrogen peroxide.

[0027] (2) Continue to add 100 μL of buffer solution (sodium dihydrogen phosphate-dipotassium hydrogen phosphate buffer solution with a concentration of 10 mmol / L, pH 7) to the mixed solution containing hydrogen peroxide obtained in step (1) to dilute 1000 times Nano-silver particle solution (particle size is about 20 nm, the original solution is made by reducing silver nitrate with sodium borohydride), hydrogen per...

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Abstract

The invention discloses a visual optical sensing method for detection of hydrogen peroxide based on nano-silver Tyndall effect. The method includes: subjecting a solution containing hydrogen peroxideand a nano-silver material solution to mixed reaction; and observing the Tyndall effect optical signal of the mixed solution under the laser beam produced by a laser pen or laser. The signal strengthis in negative correlation with the concentration of hydrogen peroxide. Low-cost and portable equipment is employed for signal (Tyndall effect optical signal) reading, and low-cost and portable quantitative detection of hydrogen peroxide or analytes (substances that produce hydrogen peroxide in a solution through specific recognition reaction or reduce the concentration of added hydrogen peroxide)related to the concentration of hydrogen peroxide can be achieved. The method provided by the invention has the outstanding advantages of simple operation, low cost, no need for professional analytical instrument, suitability for field analysis and instant detection, etc., and can be directly popularized and applied to medical diagnosis, environmental monitoring, food safety and other fields.

Description

technical field [0001] The invention belongs to the technical field of nano-sensing, and in particular relates to a visual optical sensing method for detecting hydrogen peroxide based on nano-silver Tyndall effect. Background technique [0002] The liquid-comparative colorimetric analysis method using nano-silver particles, nano-silver rods, nano-silver clusters and other nano-silver materials as colorimetric probes has the advantages of simple operation, low cost, and the analyte-mediated agglomeration of nano-silver materials makes the color of the solution change to the naked eye. Visible changes can achieve visual qualitative or semi-quantitative detection and other outstanding advantages. It has long been favored by analysts and has been widely used in medical testing, environmental analysis, food safety and other fields. However, due to the limited sensitivity of the naked eye to observe the color change of the solution when the nano-silver material is agglomerated, th...

Claims

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Application Information

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IPC IPC(8): G01N21/27
CPCG01N21/27
Inventor 聂瑾芳王兵月张云肖文成
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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