Real-time fluorescence quantification PCR detection primer of Honghu lake mysidium, kit and detection method

Abstract

The invention discloses a real-time fluorescence quantification PCR detection primer of Honghu lake mysidium. The invention further discloses a real-time fluorescence quantification PCR detection kitof Honghu lake mysidium and a detection method thereof. The primer, the kit and the detection method disclosed by the invention have high flexibility, strong specificity, good repeatability, quicknessand convenience on detecting Honghu lake mysidium and can detect 302 copies at the minimum extent. The primer, the kit and the detection method not only can detect out Honghu lake mysidium from tissues of infected fish, but also can detect out Honghu lake mysidium from environmental samples of a water sample, a mud sample and the like of a pond with disease all the year around; thus, spatiotemporal dynamic quantitative monitoring on the whole life history stage of the Honghu lake mysidium in the cultivation environment is achieved. The primer, the kit and the detection disclosed by the invention can provide technological support for early rapid diagnosis, monitoring, epidemiological investigation, fingerling production area quarantine, throat sporozoosis occurrence risk assessment and early warning, pharmacological effect assessment and reasonable control countermeasure making of crucian carp throat sporozoosis.

Description

technical field [0001] The invention relates to the field of fish parasite detection, in particular to a real-time fluorescence quantitative PCR detection primer, a detection kit and a detection method for Honghu iodine parasite. Background technique [0002] Myxozoa are a class of metazoan parasites that mainly parasitize fish, and to a lesser extent amphibians, reptiles, birds and mammals. According to literature records, there are more than 40 species of myxospores that parasitize heterogeneous gibel carp, and more than 10 species can cause diseases. Allogenetic gibel carp is a new breeding object for crucian carp successfully developed by fish breeding experts from the Institute of Hydrobiology, Chinese Academy of Sciences in 1976-1981. The male parent, the offspring bred by artificial insemination. Because of its advantages of fast growth, easy feeding, strong stress resistance and delicious meat, it is an important species of crucian carp cultured in my country. In ...

AI Technical Summary

Problems solved by technology

Although these methods can achieve the purpose of detection, there are still some shortcomings, such as high missed diagnosis rate, prone to false positives, cumbersome operation, easy contamination, relatively low sensitivity, and long time-consuming, etc. degree to quantify

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