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Arbuscular mycorrhizal fungus bacterial agent as well as preparation method and application thereof

A technology for arbuscular mycorrhizal fungi and inoculants, which are applied in the field of arbuscular mycorrhizal fungi inoculants and their preparation, to achieve the effects of promoting plant growth, good water retention and reducing toxicity

Active Publication Date: 2018-08-21
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above articles or published invention patents do not involve the use of arbuscular mycorrhizal fungi under conditions of heavy molybdenum pollution, and the use of arbuscular mycorrhizal fungi to promote the growth of corn under heavy molybdenum pollution conditions has not yet been reported or patented.

Method used

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  • Arbuscular mycorrhizal fungus bacterial agent as well as preparation method and application thereof
  • Arbuscular mycorrhizal fungus bacterial agent as well as preparation method and application thereof
  • Arbuscular mycorrhizal fungus bacterial agent as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0060] (1) Collect the rhizosphere soil of a plant in a molybdenum mining area in Luoyang, and separate the healthy mature spores of arbuscular mycorrhizal fungi in the rhizosphere soil by wet sieving, and inoculate the arbuscular mycorrhizal fungi spores into the corn seedlings after 2 weeks of sowing. On the root system, the maize seedlings after inoculation were grown on the culture medium (the mass ratio of fine river sand and bagasse is 5:1, the thickness of the culture medium is 13cm) for 3.5 months, and the Hoagland nutrition is watered every 2 weeks during the growth process. Then remove the above-ground part of the corn, separate the culture medium and the corn root system, cut the corn root system, and then mix it with the culture medium again to obtain the arbuscular mycorrhizal fungus original inoculum, which contains arbuscular mycorrhizal fungi Spores, hyphae and the culture medium of infected roots;

[0061] (2) Inoculate the arbuscular mycorrhizal fungi stock inoc...

Embodiment 2

[0064] (1) Collect the rhizosphere soil of a plant in a molybdenum mining area in Luoyang, and separate the healthy mature spores of arbuscular mycorrhizal fungi in the rhizosphere soil by wet sieving, and inoculate the arbuscular mycorrhizal fungi spores into the corn seedlings after 2 weeks of sowing. On the root system, the inoculated maize seedlings are grown on the culture medium (the ratio of fine river sand to bagasse is 4:1, and the thickness of the culture medium is 10cm) for 3 months, and the Hoagland nutrition is watered once every 2 weeks during the growth process. Then remove the above-ground part of the corn, separate the culture medium and the corn root system, cut the corn root system, and then mix it with the culture medium again to obtain the arbuscular mycorrhizal fungus original inoculum, which contains arbuscular mycorrhizal fungi Spores, hyphae and the culture medium of infected roots;

[0065] (2) Inoculate the arbuscular mycorrhizal fungi stock inoculum pr...

Embodiment 3

[0068] (1) Collect the rhizosphere soil of a plant in a molybdenum mining area in Luoyang, and separate the healthy mature spores of arbuscular mycorrhizal fungi in the rhizosphere soil by wet sieving, and inoculate the arbuscular mycorrhizal fungi spores into the corn seedlings after 2 weeks of sowing. On the root system, the maize seedlings after inoculation are grown on the culture medium (the mass ratio of fine river sand and bagasse is 6:1, the thickness of the culture medium is 15cm) for 4 months, and the Hoagland nutrition is watered once every 2 weeks during the growth process. Then remove the above-ground part of the corn, separate the culture medium and the corn root system, cut the corn root system, and then mix it with the culture medium again to obtain the arbuscular mycorrhizal fungus original inoculum, which contains arbuscular mycorrhizal fungi Spores, hyphae and the culture medium of infected roots;

[0069] (2) Inoculate the arbuscular mycorrhizal fungi stock in...

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Abstract

The invention relates to the technical fields of microbial preparations and plant cultivation, and in particular relates to an arbuscular mycorrhizal fungus bacterial agent as well as a preparation method and application thereof. The preparation method is characterized in that rhizosphere soils of molybdenum ore area plants are collected, mature spores of arbuscular mycorrhizal fungi are separatedby using a wet screening method, and then are inoculated into roots of corn seedlings; the inoculated corn seedlings are grown in a culture substrate for 3-4 months, the culture substrate and corn root systems are separated, the corn root systems are cut into pieces, and then are mixed with the culture substrate to obtain an arbuscular mycorrhizal fungus original species bacterial agent; and theoriginal species bacterial agent is inoculated into an expanding propagation substrate, corn seeds are sowed, the corns are cultured for 6-10 weeks, the expanding propagation substrate and corn root systems are separated, the corn root systems are cut into pieces, and then are mixed with the expanding propagation substrate to obtain the arbuscular mycorrhizal fungus bacterial agent. The bacterialagent can promote growth of corns in molybdenum-contaminated soils.

Description

Technical field [0001] The invention relates to the technical field of microbial preparations and plant cultivation, in particular to an arbuscular mycorrhizal fungal inoculum and a preparation method and application thereof. Background technique [0002] Molybdenum is not only one of the nutrients necessary for the growth of animals and plants, but also as a rare element, widely used in steel, electronics, aerospace and other industries, and is a very important strategic resource. my country's molybdenum reserves rank among the top in the world. There are molybdenum mines in Liaoning, Shaanxi, Shanxi, Henan, Fujian, Jiangxi and other provinces, with large reserves and good development conditions. The tailings and mining wastewater produced by the mining and dressing of molybdenum ore and associated molybdenum ore can cause serious molybdenum pollution in water bodies and soil. Since Ferguson et al. first reported that ruminants were poisoned by molybdenum caused by eating high ...

Claims

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Application Information

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IPC IPC(8): A01G18/10A01G18/20A01G31/00A01G24/10A01G24/27A01G22/20B09C1/10
Inventor 石兆勇张家成徐寿霞尹可敬李珂徐晓峰
Owner HENAN UNIV OF SCI & TECH
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