Reagent capable of detecting tumors and realizing auxiliary imaging and preparation method thereof

A tumor and imaging technology, applied in the field of biomedicine, to achieve the effect of increasing the penetration depth

Inactive Publication Date: 2018-08-24
邱军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection of nitric oxide in vivo using photoacoustic imaging technology is rarely reported.

Method used

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  • Reagent capable of detecting tumors and realizing auxiliary imaging and preparation method thereof
  • Reagent capable of detecting tumors and realizing auxiliary imaging and preparation method thereof
  • Reagent capable of detecting tumors and realizing auxiliary imaging and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0028] The preparation method of the reagent for detecting tumor and assisting imaging provided by the present invention comprises the following steps:

[0029] Dissolve 4-nitrophthalic anhydride, phthalimide, EDTA and 9-(o-carboxyphenyl)-6-hydroxy-3H-xanthene-3-one at a molar ratio of 5.8:4:0 .5:12 in ethylene glycol solution, followed by rotary evaporation to form crystals or films;

[0030] Then add 0.2 mg / ml of specific antibody PD-1 phosphate buffered saline solution, freeze the mixture, and then thaw it at 5.85°C;

[0031] Filter the solution after five times of freezing and thawing on a 200-nm filter membrane for ~50 times, and then purify it through an S-300 gel column to remove free 9-(o-carboxyphenyl)-6-hydroxy-3H- Ton-3-one and specific antibody PD-1.

[0032] The reagents, instruments or biological materials used in the reagents, instruments or biological materials used in the reagents, instruments or biological materials used in the tumor detection and imaging a...

Embodiment 1

[0034] Example 1: Preparation of reagents for detecting tumors and assisting imaging:

[0035] The reagents for detecting tumors and assisting imaging described in the present invention include a fluorescein-sensing agent characterized in that the fluorescein-sensing agent is composed of a specific fluorescein-sensing agent, a specific antibody PD-1 and 9-(o-carboxyphenyl) -6-hydroxy-3H-xanthene-3-one composition; the specific fluorescein sensor includes 4-nitrophthalic anhydride, phthalimide and EDTA; the outer layer of the specific fluorescein sensor A specific antibody PD-1 is set, and 9-(o-carboxyphenyl)-6-hydroxy-3H-xanthene-3-one is set in the inner layer of the specific fluorescein sensor.

[0036] The specific preparation method is based on a specific fluorescein sensor, and the specific antibody PD-1 is set in the outer shell, and the inner layer is used to set 9-(o-carboxyphenyl)-6-hydroxy-3H- Ton-3-one, etc., to form a fluorescein-sensing agent. Specifically inclu...

Embodiment 2

[0039] Example 2: Specificity of the Nitric Oxide Response of Fluorescein Sensing Agents

[0040] In order to study the specificity of the nitric oxide response of the fluorescein-sensing agent, in this example, the fluorescein-sensing agent and EDTA were incubated with different reactive oxygen species, including nitric oxide (NO), tert-butyl nitric oxide, hypochlorite (-OCl), hydroxyl radical (·OH), tert-butyl radical (·OtBu), nitric oxide radical (NO·), peroxide radical (O2-).

[0041] Absorption at 800 nm was measured after incubation with the above-mentioned fluorescein-sensing reagents with different reactive oxygen species for half an hour. It can be seen that the fluorescein sensor does not respond to almost all reactive oxygen species. The absorption of fluorescein-sensing agents at 800nm ​​was significantly enhanced after incubation with nitric oxide, but the response to other types of reactive oxygen species was not very strong. Therefore, the fluorescein-sensing ...

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Abstract

The invention relates to the technical field of biological medicines and particularly relates to a reagent capable of detecting tumors and realizing auxiliary imaging and a preparation method thereof.The reagent is prepared from a specific fluorescein induction agent, a specific antibody PD-1 and 9-(o-carboxyphenyl)-6-hydroxy-3H-xanthene-3-one, wherein the specific fluorescein induction agent isprepared from 4-nitrophthalic anhydride, naphthalimide and EDTA (Ethylene Diamine Tetraacetic Acid); the specific antibody PD-1 is arranged at the outer layer of the specific fluorescein induction agent; and the 9-(o-carboxyphenyl)-6-hydroxy-3H-xanthene-3-one is arranged at the inner layer of the specific fluorescein induction agent. A nano probe of the specific fluorescein induction agent can beenriched to a tumor part, can well lock the tumor under the irradiation of near-infrared light, and can not cause loss to other parts. The reagent and the preparation method thereof provided by the invention can be applied to the medical field and have profound significance.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a reagent for detecting tumors and assisting imaging, a preparation method and application thereof. Background technique [0002] As an important messenger substance and effector molecule in the body, nitric oxide (Nitric oxide, NO) participates in many physiological and pathological reactions and has a wide range of physiological functions. However, since NO is a gas free radical, it has the characteristics of fast diffusion in cells and tissues and reaction with biological targets. Therefore, it is very difficult to detect the formation and migration of NO in vivo. Therefore, it has always been a hot topic for researchers to search for a real-time, fast and simple method to detect NO. Among the numerous NO detection methods, the fluorescent probe method has the advantages of non-invasiveness, high sensitivity, good selection, and real-time monitoring and observation of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22
CPCA61K49/225
Inventor 邱军周长赞许文智
Owner 邱军
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