Application of novel lactobacillus fermentum to food fields
A fermented lactobacillus, a new technology, applied in the application field of the new fermented lactobacillus in the field of food materials, can solve problems such as low nutritional value and poor taste, achieve rich taste, rich taste and nutritional needs, and enhance immunity. force effect
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Embodiment 1
[0054] Acid resistance test
[0055] Experimental process: LBF128 lactic acid bacteria were cultivated with diluted agar medium in a petri dish, and the content of LBF128 lactic acid bacteria in the petri dish was adjusted to 10 7 / mL, the pH values of the medium were adjusted to 3.0 and 2.0 with dilute hydrochloric acid, respectively. After cultivating for 1 hour and 2 hours, respectively detect the content of LBF128 lactic acid bacteria in the petri dish, the results are as follows:
[0056] Experimental results
[0057] The acid resistance test result of table 1 lactobacillus
[0058]
[0059] The above experiments prove that LBF128 lactic acid bacteria have strong acid resistance and can survive for more than 2 hours in an environment of pH=2.0. Under acidic conditions of pH=3.0, LBF128 lactic acid bacteria can not only survive but also maintain reproductive capacity. Therefore, it is considered that it can basically pass through the strongly acidic stomach enviro...
Embodiment 2
[0061] Salt tolerance test
[0062] Experimental process: LBF128 lactic acid bacteria were cultivated with diluted agar medium in a petri dish, and the content of LBF128 lactic acid bacteria in the petri dish was adjusted to 10 7 / mL, then adjust the saline environment containing salinity 6% (mass concentration) with salt, cultivate the LBF128 lactic acid bacteria content in the culture dish respectively after 2 hours and 4 hours, the results are as follows:
[0063] Salt tolerance experiment result of table 2 lactobacillus
[0064]
[0065] The above experiment proves that LBF128 lactic acid bacteria has a strong salt resistance ability, and can survive for more than 4 hours in an environment with a salt concentration as high as 6%, indicating that the LBF128 lactic acid bacteria of the present invention can survive and work in high-salinity water such as seawater.
Embodiment 3
[0067] High temperature test
[0068] Experimental process: LBF128 lactic acid bacteria were cultivated with diluted agar medium in a petri dish, and the content of LBF128 lactic acid bacteria in the petri dish was adjusted to 10 7 / mL, then place the petri dish in an environment of 60°C and 80°C for cultivation, and detect the content of LBF128 lactic acid bacteria in the petri dish after cultivating for 1 hour and 2 hours, the results are as follows:
[0069] Salt tolerance experiment result of table 3 lactobacillus
[0070]
[0071] The above experiments prove that LBF128 lactic acid bacteria have a strong ability to resist high temperature, and can still survive and work in a high temperature environment of 80°C.
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