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Method for preparing omega-3 fatty acid-rich phospholipid from algal oil

A kind of technology of fatty acid phospholipid and algal oil phospholipid, applied in the field of health food

Inactive Publication Date: 2018-09-04
富诺健康股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2011, Professor Xuebing Xu of Aarhus University in Denmark studied the biomarker of CALA lipase and found that: under ethanol conditions, the enzyme can hydrolyze the 8 carbon chains and oleic acid of oil; but they did not further use the enzyme for its omega-3 Comprehensive analysis of fatty acid selectivity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1d) selecting Schizochytrium for cultivation and fermentation; the cultivation and fermentation are algae cultivation and heterotrophic fermentation to obtain microalgae raw materials;

[0049] 1e) drying; drying the microalgae raw material obtained in step (1) to obtain Schizochytrium powder;

[0050] 1f) wall-breaking pretreatment; the Schizochytrium powder obtained in step (2) is subjected to microalgae wall-breaking pretreatment by ultrasonic treatment to obtain the Schizochytrium dry powder.

[0051] 1c) algae oil extraction;

[0052] Take 10g of Schizochytrium algae powder, use ethanol with a mass fraction of 60-100% as the extraction agent, and use an accelerated solvent extraction device to extract, place the extract in a vacuum rotary evaporator to evaporate the organic solution, and the residue obtained after evaporation is algae oil.

[0053] 1a) Decolorization and deodorization: Add the obtained algae oil to activated clay accounting for 5% of the oil body...

Embodiment 2

[0078] 1d) Selecting Cryptidium coirii for cultivation and fermentation; said cultivation and fermentation are algae cultivation and heterotrophic fermentation to obtain microalgae raw materials;

[0079] 1e) drying; the microalgae raw material obtained in step (1) is dried to obtain Cryptidinium koii powder;

[0080] 1f) wall-breaking pretreatment; the Schizochytrium powder obtained in step (2) is subjected to microalgae wall-breaking pretreatment by ultrasonic treatment to obtain Cryptidinium koi-style dry powder.

[0081] 1c) algae oil extraction;

[0082] Take 13g of Cryptidium coirii algae powder, use mass fraction as 75% ethanol as extractant, utilize accelerated solvent extractor to extract, extract liquid is placed in vacuum rotary evaporator and organic solution is evaporated, and the residue obtained after evaporation is algae oil.

[0083] 1a) Decolorization and deodorization: Add the obtained algae oil to activated clay accounting for 5% of the oil body mass for d...

Embodiment 3

[0108] 1d) selecting Isochrysis to cultivate and ferment; said culturing and fermenting are algae culturing and heterotrophic fermentation to obtain microalgae raw materials;

[0109] 1e) drying; drying the microalgae raw material obtained in step (1) to obtain Isochrysis powder;

[0110] 1f) wall-breaking pretreatment; the Schizochytrium powder obtained in step (2) is subjected to microalgae wall-breaking pretreatment by ultrasonic treatment to obtain Isochrysis dry powder.

[0111] 1c) algae oil extraction;

[0112] Take 15g of Isochrysis algae powder, use ethanol with a mass fraction of 60-100% as the extraction agent, and use an accelerated solvent extraction apparatus to extract, and place the extract in a vacuum rotary evaporator to evaporate the organic solution, and the residue obtained after evaporation is algae oil .

[0113] 1a) Decolorization and deodorization: Add the obtained algae oil to activated clay accounting for 5% of the oil body mass for decolorization....

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PUM

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Abstract

The invention discloses a method for preparing an omega-3 fatty acid-rich phospholipid from algal oil. The method comprises 1) acquiring algal oil, 2) separating algal oil phospholipid through addingfood grade acetone into the algae oil, carrying out full mixing for 45-60min, carrying out centrifugation at 4000-5000 rpm / min for 15-20 min to obtain precipitates which are crude algal oil phospholipid and repeating the above processes, 3) purifying phosphatidylcholine through eluting a chromatographic column through ethanol for a silica gel chromatographic column to separate and remove phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol and phosphatidic acid and then separating and collecting phosphatidylcholine through elution of the chromatographic column with ethanol, and 4) synthesizing omega-3 fatty acid-rich phospholipid through mixing the extracted crude algal oil phospholipid or phosphatidylcholine and ethanol, adding lipase and an appropriate amount of water intothe mixture and preparing the omega-3 fatty acid-rich phospholipid through the lipase alcoholysis reaction. When the phosphatidylcholine is used as a synthesizing raw material, the total content of the omega-3 fatty acid after the enzyme catalytic reaction can reach to 70% or more.

Description

technical field [0001] The invention relates to the technical field of health food, in particular to a method for preparing phospholipids rich in omega-3 fatty acids by using algae oil. Background technique [0002] Phospholipids (phospholipid, PL) are a class of non-polar substances with hydrophobic groups and hydrophilic groups, mainly composed of phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylserine (PS), phospholipid A mixture of acyl inositol (PI), phosphatidic acid (PS), lysophospholipid (one fatty acid removed from PE, PC, PS, PI and PS). According to the properties and functions of phospholipids, phospholipids can be used as emulsifiers, dispersants, antioxidants, lubricants, nutritional agents, etc. and are widely used in food, medicine, cosmetics and other fields. The current world produces more than 250,000 tons of emulsifiers every year, of which phospholipids account for about 20% of the total. The commercially available phospholipid emul...

Claims

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Application Information

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IPC IPC(8): C12P7/64
CPCC12P7/6436
Inventor 程彦
Owner 富诺健康股份有限公司
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