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Application of rotenone in islet protection

A technology for rotenone and protection, which is applied in medical preparations containing active ingredients, pharmaceutical formulations, metabolic diseases, etc., can solve the problems of ineffectiveness of type I diabetes and β-cell damage.

Active Publication Date: 2019-04-12
NANJING CHILDRENS HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The etiology of type I diabetes is different from that of type II diabetes (the cause of type I diabetes is the complete damage of β cells caused by various reasons; while in patients with type II diabetes, the ability of β cells to produce insulin in the body has not been lost, but the body has developed resistance to its insulin ), and the oral hypoglycemic drugs such as thiazolidinediones and glinides currently used for type 2 diabetes are only effective for type 2 diabetes patients, but not for type 1 diabetes patients
There are no reports on the use of rotenone in the treatment of type 1 diabetes

Method used

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  • Application of rotenone in islet protection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The safety evaluation of embodiment 1 rotenone (100ppm is mixed and taken in feed)

[0034] 1 Experimental materials

[0035] The C57BL / 6 species mice used in the present invention were purchased from the Experimental Animal Center of Nanjing Medical University;

[0036] The present invention uses rotenone (Rotenone, ROT, purity ≥ 95%) purchased from Sigma-Aldrich Company. 100ppm rotenone feed (100mg rotenone was evenly mixed into 400g feed powder, mixed with 600mL agar powder aqueous solution (2%, m / v) sugar to form); the feed used by the mice in the control group was a drug-free feed prepared in the same way.

[0037] 2 Experimental methods

[0038] 2.1 Animal administration method

[0039] Thirty male C57BL / 6 mice (7 weeks old, body weight 20-24g) were fed adaptively for 1 week and randomly divided into Ctrl group (n=7) and ROT group (n=7). Before the experiment, the animals had free access to food and maintained a circadian rhythm of 12 hours of light and 12 hou...

Embodiment 2

[0044] Example 2 Rotenone Reduces Random Blood Glucose Values ​​in Type I Diabetic Mice

[0045] 1 Experimental materials

[0046] The C57BL / 6 species mice used in the present invention were purchased from the Experimental Animal Center of Nanjing Medical University;

[0047] Both streptozotocin (Streptozocin, STZ, purity 98%) and sodium citrate used in the present invention were purchased from Sigma-Aldrich Company. STZ is dissolved in sodium citrate solution (pH=4.0), ready for immediate use.

[0048] 2 Experimental methods

[0049] 2.1 Type I diabetes mouse model establishment and administration method

[0050]Thirty male C57BL / 6 mice (6-8 weeks old, body weight 20-24 g) were randomly divided into Ctrl group (n=7) and experimental group (n=23). All mice in the model group were intraperitoneally injected with STZ at a dose of 50 mg / kg after 6 hours of fasting, once a day for 5 consecutive days. Correspondingly, the control group was injected with the same volume of sodi...

Embodiment 3

[0056] Example 3 Rotenone Increases Insulin Levels in Serum and Islet Tissue of Type I Diabetic Mice

[0057] 1 Experimental materials

[0058] The mouse insulin ELISA detection kit used in the present invention was purchased from Elabscience Company; Insulin antibody was purchased from ProteinTech Company; HRP-labeled GoatAnti-Rabbit secondary antibody was purchased from Beyontian Biotechnology Co., Ltd.; other biological materials and chemical materials are the same as in Example 1 .

[0059] 2 Experimental methods

[0060] 2.1 Detection of serum insulin levels by ELISA

[0061] Collect whole blood samples from mice in each group, let them stand at room temperature for 1-2 hours, centrifuge at 4000 rpm for 10 minutes, transfer the supernatant to obtain serum samples, and measure insulin levels according to the damage steps in the kit instructions.

[0062] 2.2 Western Blot and immunohistochemical methods to detect insulin levels in islet tissue

[0063] The mice in the S...

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Abstract

The invention discloses application of rotenone in pancreas islet protection, and particularly relates to the application of the rotenone in pancreas islet protection effect in diabetes mellitus typeI. The rotenone can reduce a random blood glucose level of a mouse with the diabetes mellitus type I, the fasting insulin level in serum and pancreas islet tissue of the mouse with the diabetes mellitus type I is increased, the cell apoptosis of the pancreas islet tissue of the mouse with the diabetes mellitus type I is reduced, and the cell apoptosis of mouse pancreas islet beta cell line Min 6 induced by STZ and a cell factor mixture is reduced.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to the application of rotenone in protecting islets of pancreas. It specifically relates to the application of rotenone in protecting islets in type I diabetes. technical background [0002] Pancreatic islets (langerhans) are the endocrine part of the pancreas, consisting of many cell clusters of various sizes and shapes, scattered throughout the pancreas, and can control the metabolism of carbohydrates. Human islet cells are mainly divided into α cells, β cells, γ cells and PP cells according to their staining and morphological characteristics. α cells account for about 20% of islet cells and secrete glucagon; β cells account for 60%-70% of islet cells and secrete insulin (insulin); γ cells account for 10% of islet cells and secrete "growth inhibitory hormone". The number of PP cells is very small, secreting pancreatic polypeptide (pancreaticpolypeptide); insulin is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61P5/50A61P3/10
CPCA61K31/352A61P3/10A61P5/50
Inventor 贾占军吴梦秋张爱华张玥麻昊阳陈维宜
Owner NANJING CHILDRENS HOSPITAL
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