Application of rotenone in islet protection
A technology for rotenone and protection, which is applied in medical preparations containing active ingredients, pharmaceutical formulations, metabolic diseases, etc., can solve the problems of ineffectiveness of type I diabetes and β-cell damage.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0033] The safety evaluation of embodiment 1 rotenone (100ppm is mixed and taken in feed)
[0034] 1 Experimental materials
[0035] The C57BL / 6 species mice used in the present invention were purchased from the Experimental Animal Center of Nanjing Medical University;
[0036] The present invention uses rotenone (Rotenone, ROT, purity ≥ 95%) purchased from Sigma-Aldrich Company. 100ppm rotenone feed (100mg rotenone was evenly mixed into 400g feed powder, mixed with 600mL agar powder aqueous solution (2%, m / v) sugar to form); the feed used by the mice in the control group was a drug-free feed prepared in the same way.
[0037] 2 Experimental methods
[0038] 2.1 Animal administration method
[0039] Thirty male C57BL / 6 mice (7 weeks old, body weight 20-24g) were fed adaptively for 1 week and randomly divided into Ctrl group (n=7) and ROT group (n=7). Before the experiment, the animals had free access to food and maintained a circadian rhythm of 12 hours of light and 12 hou...
Embodiment 2
[0044] Example 2 Rotenone Reduces Random Blood Glucose Values in Type I Diabetic Mice
[0045] 1 Experimental materials
[0046] The C57BL / 6 species mice used in the present invention were purchased from the Experimental Animal Center of Nanjing Medical University;
[0047] Both streptozotocin (Streptozocin, STZ, purity 98%) and sodium citrate used in the present invention were purchased from Sigma-Aldrich Company. STZ is dissolved in sodium citrate solution (pH=4.0), ready for immediate use.
[0048] 2 Experimental methods
[0049] 2.1 Type I diabetes mouse model establishment and administration method
[0050]Thirty male C57BL / 6 mice (6-8 weeks old, body weight 20-24 g) were randomly divided into Ctrl group (n=7) and experimental group (n=23). All mice in the model group were intraperitoneally injected with STZ at a dose of 50 mg / kg after 6 hours of fasting, once a day for 5 consecutive days. Correspondingly, the control group was injected with the same volume of sodi...
Embodiment 3
[0056] Example 3 Rotenone Increases Insulin Levels in Serum and Islet Tissue of Type I Diabetic Mice
[0057] 1 Experimental materials
[0058] The mouse insulin ELISA detection kit used in the present invention was purchased from Elabscience Company; Insulin antibody was purchased from ProteinTech Company; HRP-labeled GoatAnti-Rabbit secondary antibody was purchased from Beyontian Biotechnology Co., Ltd.; other biological materials and chemical materials are the same as in Example 1 .
[0059] 2 Experimental methods
[0060] 2.1 Detection of serum insulin levels by ELISA
[0061] Collect whole blood samples from mice in each group, let them stand at room temperature for 1-2 hours, centrifuge at 4000 rpm for 10 minutes, transfer the supernatant to obtain serum samples, and measure insulin levels according to the damage steps in the kit instructions.
[0062] 2.2 Western Blot and immunohistochemical methods to detect insulin levels in islet tissue
[0063] The mice in the S...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com