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Method for separating and preparing petunidin 3-galactoside chloride

A technology for petunin and galactoside, which is applied in the field of separation and preparation of petunin-3-O-galactoside, and can solve the problem of separation and preparation of petunin-3-O-galactoside that has not been found. Issues such as body research and reporting, to achieve the effect of large sample processing volume and good repeatability

Active Publication Date: 2018-09-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] However, there is no research and report on the isolation and preparation of petunienin-3-O-galactoside monomers from blueberries

Method used

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  • Method for separating and preparing petunidin 3-galactoside chloride
  • Method for separating and preparing petunidin 3-galactoside chloride
  • Method for separating and preparing petunidin 3-galactoside chloride

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] (1) Wash 1000g of fresh blueberries, add 70% ethanol aqueous solution containing 0.1% (v / v) hydrochloric acid (volume of ethanol and water) according to the ratio of solid to liquid 1:7 (w / v, g / mL) Ratio of 70:30) was fully mixed, ultrasonically extracted for 90 minutes, (45°C, protected from light), vacuum filtered, the filter residue was extracted twice according to the above conditions, the filtrate was combined, and ethanol was removed by vacuum rotary evaporation at 48°C to obtain crude anthocyanins extract;

[0070] (2) The AB-8 macroporous resin soaked in ethanol for 24h is packed into a chromatographic column, washed with deionized water until there is no alcohol smell, washed with 4% hydrochloric acid solution for 1h at a flow rate of 2BV / h, and then deionized Wash with ionized water until the effluent is neutral; wash with 4% sodium hydroxide solution at a flow rate of 2BV / h for 1 hour, and then wash with deionized water until neutral. Inject the crude anthoc...

Embodiment 2

[0076] (1) Wash 3kg of fresh blueberries, add 80% ethanol aqueous solution containing 1.0% (v / v) hydrochloric acid according to the ratio of solid to liquid 1:9 (w / v) and mix thoroughly, ultrasonically extract for 120min, (45°C hereinafter, protected from light), filtered under reduced pressure, and the filter residue was repeatedly extracted 3 times according to the above conditions, and the filtrate was combined, and the ethanol was removed by vacuum rotary evaporation at 46°C to obtain a crude anthocyanin extract;

[0077] (2) The AB-8 macroporous resin soaked in ethanol for 24h is packed into a chromatographic column, washed with deionized water until there is no alcohol smell, washed with 4% hydrochloric acid solution for 1h at a flow rate of 2BV / h, and then deionized Wash with ionized water until the effluent is neutral; wash with 4% sodium hydroxide solution at a flow rate of 2BV / h for 1 hour, and then wash with deionized water until neutral. Inject the crude anthocyani...

Embodiment 3

[0082] (1) Wash 5kg of fresh blueberries, add 90% ethanol aqueous solution containing 1.5% (v / v) hydrochloric acid according to the ratio of material to liquid (w / v) of 1:8 (w / v), mix thoroughly, and extract by ultrasonic for 200min, (45°C hereinafter, protected from light), filtered under reduced pressure, and the filter residue was repeatedly extracted 4 times according to the above conditions, the filtrates were combined, and the ethanol was removed by vacuum rotary evaporation at 47°C to obtain a crude anthocyanin extract;

[0083] (2) The AB-8 macroporous resin soaked in ethanol for 24h is packed into a chromatographic column, washed with deionized water until there is no alcohol smell, washed with 4% hydrochloric acid solution for 1h at a flow rate of 2BV / h, and then deionized Wash with ionized water until the effluent is neutral; wash with 4% sodium hydroxide solution at a flow rate of 2BV / h for 1 hour, and then wash with deionized water until neutral. Inject the crude an...

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Abstract

The invention provides a method for separating and preparing petunidin 3-galactoside chloride. The method comprises alcohol extraction and concentration, macroporous resin adsorption, preparative liquid chromatography purification and high-speed counter-current chromatography separation, and a high-purity petunidin 3-galactoside chloride monomer is isolated and prepared from blueberry raw materials with complicated composition of anthocyanin. The method combines preparative liquid chromatography and high-speed counter-current chromatography technologies for the first time, the high-purity petunidin 3-galactoside chloride monomer is isolated and obtained from blueberries by optimizing process parameters, and the purity is up to 99%.

Description

technical field [0001] The invention relates to the field of separation and purification of natural products, in particular to a method for the separation and preparation of petunienin-3-O-galactoside. Background technique [0002] Anthocyanin is a water-soluble pigment widely present in plants. It is a polyphenol compound formed by combining anthocyanin with one or more sugar groups, such as glucose, galactose, and arabinose, through glycosidic bonds. There are mainly 6 kinds of anthocyanins commonly found in nature, namely Pelargonidin, Cyaniain, Delphinidin, Peonidin, Petunidin and Malva pigment (Malvidin). In recent years, a large number of studies have confirmed that anthocyanins from natural sources have biological activities such as anti-oxidation, anti-tumor, prevention of cardiovascular diseases, alleviation of diabetes and obesity control. With the continuous improvement of people's living standards, people's calls for functional components from natural sources w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/065C07H1/08
CPCC07H1/08C07H17/065
Inventor 陈卫徐阳谢佳宏
Owner ZHEJIANG UNIV
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