Polysaccharide and application in curing lung cancer as BCAT1 gene silencing agent

Abstract

The invention discloses a polysaccharide and application in curing lung cancer as a BCAT1 gene silencing agent. The polysaccharide is prepared through the following steps that 1, amomum tsao-ko is crushed, and through aqueous extraction, deproteinization and alcohol precipitation, a crude amomum tsao-ko polysaccharide is obtained; 2, the crude amomum tsao-ko polysaccharide is loaded and dissolvedin DEAE-52 cellulose chromatogram and subjected to gradient elution through distilled water, a 0.1 mol / L NaCl solution, a 0.3 mol / L NaCl solution, a 0.5 mol / L NaCl solution, a 0.7 mol / L NaCl solutionand a 0.9 mol / L NaCl solution, 0.9 mol / L NaCl solution eluant is collected, and dialysis, concentration and drying are conducted; 3, after Sephacryl S400-type gel filler is soaked in pure water overnight for full swelling, packing is conducted to prepare a 0.9 mol / L NaCl solution eluant solution for sample loading, a 0.1 mol / L ammonium bicarbonate solution is used as eluant, the flow velocity is 0.3 mL / min, every 6 mL is collected in one tube, polysaccharide efflux is tracked and detected through a sulfuric acid-phenol method, the number of the tubes is regarded as the abscissa, the light absorption value is regarded as the ordinate, a peak spectrum of the polysaccharide is drawn, according to the spectrum, flow samples corresponding to target chromatographic peaks are combined, and through concentration and drying, the refined polysaccharide E is obtained.

Description

technical field [0001] The invention belongs to the biological field and relates to the preparation and application of polysaccharides, in particular to a polysaccharide and its use as a BCAT1 gene silencer for treating lung cancer. Background technique [0002] The proliferation of cells needs to obtain amino acids to synthesize proteins or use them as nitrogen sources to synthesize nucleotides and non-essential amino acids. Studies have shown that non-small cell lung cancer relies more on branched-chain amino acids to obtain nitrogen sources than normal tissues. Branched-chain amino acid transaminase 1 (BCAT1) is an initiator of branched-chain amino acid metabolism and an important regulator of the tricarboxylic acid cycle and oxidative phosphorylation, which is critical for cell growth and energy production. [0003] Studies have shown that silencing BCAT1 reduces cellular oxygen intake and affects glycolytic pathways, reducing cellular energy production. More and more ...

AI Technical Summary

Problems solved by technology

[0005] Tsaoko contains a relatively high content of polysaccharides, but the prior art does not record the application of Tsaoko polysaccharides in tumor treatment

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