A kind of breeding method of tomato disease-resistant homozygous

A cultivation method and homozygous technology, applied in the field of crop breeding, can solve the problems of low breeding success rate and long breeding cycle, and achieve the effects of improving the survival rate, weak photosynthetic autotrophic ability, and improving the survival rate of seedlings.

Active Publication Date: 2021-06-04
SHANDONG SHOUGUANG VEGETABLE SEED IND GRP CO LTD +1
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Problems solved by technology

[0003] In traditional breeding, the acquisition of high-generation pure and inbred line parent materials that are resistant to various diseases mainly uses self-crossing, hybridization and backcrossing techniques, which usually take 3 to 5 years to complete. The breeding cycle is long and the breeding success rate is low. Therefore, There is an urgent need to establish a complete set of efficient breeding system to improve breeding efficiency and effectively serve production

Method used

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  • A kind of breeding method of tomato disease-resistant homozygous
  • A kind of breeding method of tomato disease-resistant homozygous
  • A kind of breeding method of tomato disease-resistant homozygous

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Take tomato anthers as explants, set up 6 kinds of different mediums to deal with, each repeat 3 times, the specific technical scheme is as follows:

[0045] Treatment 1: Carry out tomato anther haploid culture according to the method of the present invention.

[0046] On a sunny day, 6-8 mm long flower buds selected from the disease-resistant tomato mutant plants in the greenhouse of the breeding base of Shouguang Vegetable Group were collected and pretreated at 4°C for 4 days. Before inoculation, the flower buds were sterilized with 75% alcohol for 30 seconds, then sterilized with 0.1% mercuric chloride for 10 minutes, and then washed 4 times with sterile water. The anthers were taken out from the flower buds under aseptic conditions, and the anthers with a length of 2-5 mm (the pollen development period in them was the uninucleate marginal stage) were selected for anther culture. Inoculate the anthers on MS basic medium, add KT 0.5mg / L, 2,4-D 1mg / L, trehalose 30g / L,...

Embodiment 2

[0073] Practical application: Carry out tomato anther haploid culture according to the method of the present invention.

[0074] On sunny days, flower buds with a length of 6-8 mm were collected from the disease-resistant tomato mutant plants in the greenhouse of the Shouguang Vegetable Group Breeding Base, and pretreated at 4°C for 4 days. Before inoculation, the flower buds were sterilized with 75% alcohol for 30 seconds, then sterilized with 0.1% mercuric chloride for 10 minutes, and then washed 4 times with sterile water. The anthers were taken out from the flower buds under aseptic conditions, and the anthers with a length of 2-5 mm (the pollen development period in them was the uninucleate marginal stage) were selected for anther culture. The anthers were inoculated on MS basic medium, KT0.5mg / L, 2,4-D 1mg / L, trehalose 30g / L, black wolfberry juice filtrate 20g / L and agar 8g / L, pH 5.8 callus induction culture. Culture conditions: relative humidity 80%, 24-26°C, light in...

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Abstract

The invention discloses a method for cultivating tomato disease-resistant homozygotes. The method includes a tissue culture identification step and a disease resistance screening step. The tissue culture is based on a conventional medium formula by using trehalose instead of sucrose and adding black wolfberry juice for filtration. The specific medium formula prepared from the liquid nutrient components was used to prepare tomato explants, anther callus induction culture, callus culture, complete plant acquisition, colchicine treatment and ploidy identification to obtain homozygous seedlings ; and combined with molecular marker technology for disease-resistant molecular marker screening, to obtain disease-resistant homozygous plants. The method of the invention can significantly shorten the screening period, and quickly obtain tomato disease-resistant homozygotes; and the method is simple in operation, low in cost, high in survival rate after transplanting flower seedlings, strong in stress resistance, easy to popularize, and can be directly applied to production. It has good economic and social benefits.

Description

technical field [0001] The invention relates to the technical field of crop breeding, in particular to a method for cultivating tomato disease-resistant homozygotes. Background technique [0002] Tomato is an annual or perennial plant of the Solanaceae family, and it is one of the most important vegetable crops in the world. For a long time, various tomato diseases have always been a huge threat to production. Especially since the 1960s, with the widespread planting of tomatoes all over the country, various diseases have spread rapidly and increased year by year. The breeding of disease-resistant varieties is the most effective way of prevention and control with significant economic and ecological benefits. [0003] In traditional breeding, the acquisition of high-generation pure and inbred line parent materials that are resistant to various diseases mainly uses self-crossing, hybridization and backcrossing techniques, which usually take 3 to 5 years to complete. The breedi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 程琳武玉芬国家进潘子龙胡永军于彩云许勇王茂昌李晓杰
Owner SHANDONG SHOUGUANG VEGETABLE SEED IND GRP CO LTD
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