Method for treating waste fermented liquid of molasses by utilizing microalgae

A technology for molasses waste mash and microalgae, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of increasing production costs, corrosion of carbon steel equipment, consuming large energy, etc., and achieves the improvement of resource utilization. Utilization rate, realization of waste utilization, and production cost savings

Inactive Publication Date: 2018-09-14
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the wastewater treated by anaerobic digestion can meet the discharge standard, the anaerobic digestion process increases the production cost. At the same time, most of the organic matter in the wastewater is decomposed and digested in the air, resulting in a waste of energy.
[0004] Utilize the high concentration of organic matter inherent in molasses waste mash to produce biogas through fermentation, but its nitrogen, phosphorus, and potassium remain in the biogas slurry and biogas residue containing a large amount of transformed organic matter, which is easy to cause soil acidification and compaction
At the same time, the fermentation process has a pungent smell and pollutes the air
Molasses waste mash can also be treated by evaporation and concentration, but it consumes a lot of energy during the concentration process, and because the waste water contains acid ions such as sulfate, chloride, and organic acids, it will seriously corrode carbon steel equipment.
Therefore, the molasses waste mash is treated by evaporation and concentration, and the one-time investment is relatively large.

Method used

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  • Method for treating waste fermented liquid of molasses by utilizing microalgae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Dilute the molasses waste mash 1600 times, as the Monoraphidium The basal medium for FXY-10 culture, while adding 83.80mg / L of MgSO 4 ·7H 2 O, get Mg 2+ Medium with a concentration of 400μmol / L; adjust the pH value between 6.8-7.0 with NaOH (1mol / L), sterilize at 121°C for 20min, and inoculate Monospirulina Monoraphidium FXY-10 was cultured in light shake flasks, the initial inoculation amount was 0.1g / L, the culture temperature was 25±1°C, the light intensity was 2500lux, and the shaker speed was 150r / min.

[0017] Centrifuge the algae fluid cultivated to the stationary phase, collect the supernatant, and measure the ion content: the method for determining the ion content in the waste molasses mash is: centrifuge the microalgae cultivated to the stable phase at 3500r / min for 5min, collect the supernatant, and use After filtering through a filter membrane of 0.22 μm, the content of each ion in the supernatant was measured by an atomic absorption spectrometer.

...

Embodiment 2

[0020] Dilute the molasses waste mash 1600 times, as the Monoraphidium The basal medium for FXY-10 culture was supplemented with 182.2 mg / L MgSO 4 ·7H 2 O, get Mg 2+ Medium with a concentration of 800 μmol / L; adjust the pH value between 6.8 and 7.0 with NaOH (1mol / L), sterilize at 121°C for 20 minutes, and inoculate Monospirulina Monoraphidium For FXY-10, the initial inoculum amount is 0.1g / L, the culture temperature is 25±1°C, the light intensity is 2500lux, and the shaker speed is 150r / min, and the light shake flask culture is carried out.

[0021] Centrifuge the algae fluid cultivated to the stationary phase, collect the supernatant, and measure the ion content: the method for determining the ion content in the waste molasses mash is: centrifuge the microalgae cultivated to the stable phase at 3500r / min for 5min, collect the supernatant, and use After filtering through a filter membrane of 0.22 μm, the content of each ion in the supernatant was measured by an atomic ...

Embodiment 3

[0024] Dilute the molasses waste mash 1600 times, as the Monoraphidium The basal medium for QLY-10 culture, while adding 280.6mg / L of MgSO 4 ·7H 2 O, get Mg 2+ Medium with a concentration of 1200 μmol / L. Use NaOH (1mol / L) to adjust the pH value between 6.8-7.0, sterilize at 121°C for 20min, and inoculate Monospinatus Monoraphidium For QLY-10, the initial inoculum amount was 0.1g / L, the culture temperature was 25±1°C, the light intensity was 2500lux, and the shaker speed was 150r / min, and the light shake flask culture was carried out.

[0025] Centrifuge the algae fluid cultivated to the stationary phase, collect the supernatant, and measure the ion content: the method for determining the ion content in the waste molasses mash is: centrifuge the microalgae cultivated to the stable phase at 3500r / min for 5min, collect the supernatant, and use After filtering through a filter membrane of 0.22 μm, the content of each ion in the supernatant was measured by an atomic absorpt...

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Abstract

The invention discloses a method for treating waste fermented liquid of molasses by utilizing microalgae, and belongs to the technical field of treating organic wastewater by utilizing the microalgae.The method comprises the following steps: diluting the waste fermented liquid of the molasses as a culture medium for culturing the microalgae, adding MgSO4.7H2O, regulating the pH (Potential of Hydrogen) value, sub-packaging, and sterilizing; inoculating the prepared culture medium with the microalgae, and carrying out illumination shake-flask culture. The method disclosed by the invention is simple and easy in operation, ions in the waste fermented liquid of the molasses can be enriched by utilizing the microalgae, self growth demands of the microalgae can be supplied, the content of the ions in the waste fermented liquid of the molasses and the production cost of microalgae culture are reduced, and the environment protection is facilitated.

Description

technical field [0001] The invention relates to a method for using microalgae to treat waste molasses mash, and belongs to the technical field of using microalgae to treat organic wastewater. Background technique [0002] Molasses waste mash is an organic waste liquid with high concentration, dark color and high acidity discharged during the production of sugar products, with a pH value of 3.0 to 5.0. It is difficult to meet the standards for the treatment of waste molasses mash, and the investment is high, and direct discharge will seriously damage the ecological balance. At the same time, the waste molasses mash contains many amino acids, proteins, sugars and mineral elements such as Na, K, Ca, Mg, P, Fe, Zn, etc. Its solid content is 8% to 10%, and organic matter accounts for about 70%. . How to economically and effectively treat alcohol waste mash is still a difficult problem in sugar factory pollution control. [0003] Because molasses waste mash contains a large amo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 余旭亚董训赞赵永腾黄力
Owner KUNMING UNIV OF SCI & TECH
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