Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Hybrid membrane strip for mycobacterium tuberculosis complex and avian-extracellular mycobacterium complex and detection kit

A Mycobacterium tuberculosis detection kit technology, applied in the field of medical testing, can solve the problems of high infection risk and infection hazards of Mycobacterium tuberculosis, and achieve the effects of saving time and equipment costs, rapid detection, and rapid selection

Active Publication Date: 2018-09-14
广州市宝创生物技术有限公司
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Mycobacterium tuberculosis, because of its high infection risk and serious infection hazards, requires us to develop a simple and effective detection system that can avoid operational risks; atypical mycobacterium tuberculosis, especially mycobacterium avium-extracellular group, due to the increasing detection rate year by year, coupled with resistance to first-line tuberculosis drugs, resulting in a completely different treatment plan from tuberculosis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hybrid membrane strip for mycobacterium tuberculosis complex and avian-extracellular mycobacterium complex and detection kit
  • Hybrid membrane strip for mycobacterium tuberculosis complex and avian-extracellular mycobacterium complex and detection kit
  • Hybrid membrane strip for mycobacterium tuberculosis complex and avian-extracellular mycobacterium complex and detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0049] Experimental samples: A total of 12 vaginal swab samples were taken from clinical experiment units in two batches. The first batch took 5 TB positive samples and 1 TB negative sample; the second batch also took 5 NG positive samples and 1 NG swab sample. negative sample.

[0050] Nucleic acid extraction: the sputum sample is fully dissolved and washed with 1000ul of sputum liquefaction agent, the sample is placed in a centrifuge at 5000rpm, centrifuged for 15 minutes, the supernatant is removed, and the resulting precipitate is extracted with a nucleic acid extraction kit (Yue Sui Machinery Equipment 20150224 No.) sample lysate, and then perform nucleic acid extraction according to the reagent instructions. Finally, use 50 microliters of distilled water to elute the nucleic acid and set aside

[0051] PCR amplification: The extracted nucleic acid is subjected to PCR amplification. The reaction system is: 2xPCR buffer 10ul, TB-MAC primer 5ul, sample DNA 5ul. Mix well. ...

Embodiment 3

[0059] Example 3: TB Specific Experiments

[0060] Experimental samples: A total of 8 vaginal swab samples were taken from the clinical experiment unit in two batches. The first batch took 3 TB positive samples and 1 TB negative sample; the second batch also took 3 TB positive samples and 1 TB negative sample sample.

[0061] Nucleic acid extraction: the sputum sample is fully dissolved and washed with 1000ul of sputum liquefaction agent, the sample is placed in a centrifuge at 5000rpm, centrifuged for 15 minutes, the supernatant is removed, and the resulting precipitate is extracted with a nucleic acid extraction kit (Yue Sui Machinery Equipment 20150224 No.) sample lysate, and then perform nucleic acid extraction according to the reagent instructions. Finally, 50 microliters of distilled water was used to elute the nucleic acid and set aside.

[0062] PCR amplification: The extracted nucleic acid is subjected to PCR amplification. The reaction system is: 2xPCR buffer 10ul,...

Embodiment 4

[0070] Embodiment 4: MAC specificity experiment

[0071] Experimental samples: A total of 12 vaginal swab samples were taken from the clinical experiment unit in two batches. The first batch took 5 MAC positive samples and 1 MAC negative sample; the second batch also took 5 MAC positive samples and 1 MAC negative sample sample.

[0072] Nucleic acid extraction: the sputum sample is fully dissolved and washed with 1000ul of sputum liquefaction agent, the sample is placed in a centrifuge at 5000rpm, centrifuged for 15 minutes, the supernatant is removed, and the resulting precipitate is extracted with a nucleic acid extraction kit (Yue Sui Machinery Equipment 20150224 No.) sample lysate, and then perform nucleic acid extraction according to the reagent instructions. Finally, 50 microliters of distilled water was used to elute the nucleic acid and set aside.

[0073] PCR amplification: The extracted nucleic acid is subjected to PCR amplification. The reaction system is: 2xPCR b...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a hybrid membrane strip for mycobacterium tuberculosis complex and avian-extracellular mycobacterium complex and a detection kit. The kit comprises a hybrid membrane strip, wherein at least one of the following probes is fixed on a nitrocellulose membrane of the hybrid membrane strip: oligonucleotide sequences which are completely complementary with SEQ ID NO.1 and SEQ ID NO.2 specified at the mycobacterium tuberculosis complex and avian-extracellular mycobacterium complex. According to the kit disclosed by the invention, by virtue of selection of special detection fragments, single-species microbes can be detected, existence of two types of pathogenic microorganisms can be detected in a PCR in the same tube, and a simple, rapid and cheap choice is provided for screening.

Description

technical field [0001] The invention belongs to the field of medical detection, and in particular relates to a hybrid membrane strip and a detection kit for mycobacterium tuberculosis complex and avium-extracellular mycobacterium complex. Background technique [0002] Tuberculosis is a major infectious disease that endangers national health, and my country is one of the main countries with a large incidence of tuberculosis. Due to the particularity of Mycobacterium tuberculosis, its detection and treatment are unanimously the bottleneck restricting human beings from overcoming tuberculosis. The traditional tuberculosis diagnosis method is based on culture technology. Due to the unique growth characteristics of Mycobacterium tuberculosis, the general culture takes 3-4 weeks to get the results. Even the most advanced liquid culture technology takes about 10 days to get the inspection results. , which brings severe challenges to the screening and confirmation of tuberculosis. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/689C12Q1/04C12N15/11
CPCC12Q1/6834C12Q1/689C12Q2600/16C12Q2565/625C12Q2537/143
Inventor 杨祥胜
Owner 广州市宝创生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com