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Preparation method, product and application of pH-responsive ultra-small iron oxide nanoparticle assembly

An iron oxide nanometer, responsive technology

Active Publication Date: 2020-08-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional method is often to use polymers to wrap ultra-small iron oxide nanoparticles to form bio-environment-responsive nanomicelle carriers. Due to the obstruction of water penetration by the thick polymer layer and the instability at extremely low concentrations, etc. factors, its in vivo imaging performance and bioavailability are greatly limited

Method used

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  • Preparation method, product and application of pH-responsive ultra-small iron oxide nanoparticle assembly
  • Preparation method, product and application of pH-responsive ultra-small iron oxide nanoparticle assembly
  • Preparation method, product and application of pH-responsive ultra-small iron oxide nanoparticle assembly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1: Synthesis of ultra-small iron oxide nanoparticles coated with oleic acid

[0058] (1) Dissolve 10 g of ferric chloride hexahydrate and 36 g of sodium oleate in a mixed solution of 80 mL of ethanol, 60 mL of water and 140 mL of hexane, and stir at 70° C. for 4 hours. After the reaction, the oil phase part is extracted with water and dried to obtain the oil-iron composite.

[0059] (2) Take 1.8g of oil-iron complex and dissolve it in 0.6g of oleic acid, 1.6g of oleyl alcohol and 10g of diphenyl ether mixed solution, raise the temperature to 250°C under the protection of argon, and keep stirring at this temperature for 30 minutes. After the reaction is cooled, add Acetone precipitates and centrifuges to obtain ultra-small iron oxide nanoparticles in the oil phase, and the size of the nanoparticles is 3-4 nm.

[0060] The morphology of the prepared oleic acid-coated ultra-small iron oxide nanoparticles was characterized by transmission electron microscopy, as sh...

Embodiment 2

[0061] Example 2: Synthesis of carboxyl-modified ultra-small iron oxide nanoparticles

[0062] 100 mg of oleic acid-modified ultra-small iron oxide nanoparticles were dissolved in 7.5 ml of o-dichlorobenzene, and 100 mg of citric acid and 7.5 ml of N,N-dimethylformamide were added. Stir at 100°C for 24 hours under the protection of argon. After the reaction, add ether and acetone for precipitation, and centrifuge to obtain carboxyl-modified ultra-small iron oxide nanoparticles.

[0063] The morphology of the prepared carboxyl-modified ultra-small iron oxide nanoparticles was characterized by transmission electron microscopy, as shown in figure 2 shown.

Embodiment 3

[0064] Example 3: Synthesis of Carboxyl-Modified Ultra-Small Iron Oxide Nanoparticles

[0065] The synthesis was carried out with reference to the preparation process of Example 2, the difference being that the added oleic acid modified ultra-small iron oxide nanoparticles was changed to 20 mg, and carboxyl-modified ultra-small iron oxide nanoparticles were also obtained.

[0066] The morphology of the carboxyl-modified ultra-small iron oxide nanoparticles prepared by changing the method was characterized by transmission electron microscopy, as shown in image 3 shown.

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Abstract

The invention relates to a preparation method of a pH-responsive ultra-small iron oxide nanoparticle assembly, a product thereof, and an application of the product. The preparation method comprises the following steps: 1) performing a ligand exchange reaction on an oleic acid coated ultra-small iron oxide nanoparticle with a small molecule having a carboxyl group to obtain a carboxyl-modified ultra-small iron oxide nanoparticle; 2) performing an amidation reaction on the carboxyl-modified ultra-small iron oxide nanoparticle and a small molecule having a hydrazino group to obtain a hydrazine-modified ultra-small iron oxide nanoparticle; and 3) performing an aldehyde-amine condensation reaction on the hydrazine-modified ultra-small iron oxide nanoparticle and a compound having a plurality ofaldehyde groups to obtain the pH-responsive ultra-small iron oxide nanoparticle assembly. The preparation method introduces the hydrophilic small-molecular ligand to make the ultra-small iron oxide nanoparticle chemical crosslinked in order to form the pH-responsive nano-assembly, so the assembly has a good bioavailability and a good imaging effect.

Description

technical field [0001] The invention relates to the field of preparation of iron oxide assemblies, in particular to a preparation method, product and application of a pH-responsive ultra-small iron oxide nanoparticle assembly. Background technique [0002] Cancer is one of the biggest killers threatening human life around the world, and it is a major challenge facing the current medical research field. According to statistics, cancer has become the first cause of death among urban residents in China. Effectively diagnosing early cancers has become a top priority in scientific research. [0003] Magnetic resonance imaging (MRI) is widely used in clinical diagnosis of cancer. In order to improve the contrast between the tumor area and the normal area, it is often necessary to introduce a contrast agent. The widely studied responsive iron oxide assembly can weaken the magnetic resonance signal of the tumor area for the purpose of comparison, but it is easily confused with ot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/18A61K49/08A61K49/10
CPCA61K49/085A61K49/10A61K49/1833
Inventor 凌代舜李方园梁泽宇
Owner ZHEJIANG UNIV
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