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A method for multi-strain combination fermentation of bacterial bran

A combined fermentation and multi-strain technology, applied to Lactobacillus, bacteria used in food preparation, animal husbandry, etc., can solve the problems of high crude fiber content, restrict animal digestion and utilization, etc., and achieve the effect of increasing the content

Active Publication Date: 2020-07-10
JILIN AGRI SCI & TECH COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacteria chaff is rich in bacterial protein, and contains growth factors such as saccharides, purines, vitamins, etc., but the content of crude fiber is still high, which limits the digestion and utilization of animals.

Method used

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  • A method for multi-strain combination fermentation of bacterial bran
  • A method for multi-strain combination fermentation of bacterial bran
  • A method for multi-strain combination fermentation of bacterial bran

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Experimental program
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Embodiment

[0038] Isolation and Screening of High Cellulase Producing Strains

[0039] Pretreatment of the sample material: After the soil sample is first heated and dried at a constant temperature of 30°C, 5.0g of the sample is added to a conical flask filled with 95mL of sterile water, fully shaken at 30°C and 200r / min for 40min, and left to stand ,spare.

[0040] Primary screening of bacterial strains: Take 1 mL of the supernatant of the stock solution and add it to a test tube filled with 9 mL of sterile water, dilute to 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 Then spread it on the cellulose plate separation medium, cultivate it in a 37°C constant temperature biochemical incubator for 24 hours, count the colonies, and then pick out the colonies with different shapes, and repeatedly inoculate them on the corresponding agar. Plate until a single colony is obtained after purification, and then transfer it to the corresponding agar slant and save it for later use.

[0041] R...

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Abstract

The invention discloses a method for fermenting fungus chaff with multi-strain combination, comprising the following steps: S1, pre-mixing and fermenting Trichoderma and Pleurotus ostreatus fungus chaff, the pH of the fermentation medium is 4.5, the inoculation amount is 5%, and the fermentation temperature is 37°C, the fermentation time is 72h; S2, after mixing Bacillus subtilis, yeast and lactic acid bacteria according to the mass ratio of 1:1:3, and then mixing and fermenting the chaff of Pleurotus ostreatus treated with Trichoderma, the pH of the fermentation medium is 7.5 , the inoculum size is 15%, the fermentation temperature is 37°C, and the fermentation time is 96h. The invention is beneficial to the improvement of the content of crude protein and the utilization of crude fiber in the fungus chaff.

Description

technical field [0001] The invention relates to the field of fungus chaff treatment, in particular to a method for fermenting fungus chaff with multi-strain combination. Background technique [0002] On average, 150 to 200 billion tons of plant-based organic matter are produced every year in the world, and more than half of them are cellulose substances, which are the most abundant resources on the earth. my country is a big country in the production of edible fungi. With the development of the edible fungus industry, the chaff produced by the cultivation of edible fungi is also increasing. Therefore, the chaff has become an important resource with a considerable amount. Because the fungus chaff is rich in bacterial protein, and contains growth factors such as bacterial sugars, purines, vitamins, etc., but the crude fiber content is still high, which limits the digestion and utilization of it by animals. However, only a small part of the fungus chaff can be used now, and mo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/12
CPCA23K10/12A23V2400/11
Inventor 邢力
Owner JILIN AGRI SCI & TECH COLLEGE