Preparation method and application of immunological enhancement compound tablets for pets
A pet and synergistic technology, applied in the field of pet medicine, can solve the problem of unable to identify mutant cells and so on
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[0015] The composite immune booster for pets of the present invention is described in detail below in conjunction with specific examples, and the preparation steps are as follows:
[0016] 1. Extraction of bursa active peptide BP-IV
[0017] Take 4kg of healthy 4-6 week-old chicken bursa, extract BP-IV, crush and mix with 6L normal saline, freeze and thaw three times, then centrifuge, take the supernatant, ultrafilter, 1000Da ultrafiltration membrane, freeze Dry filtrate; sample was diluted with PBS, and then centrifuged at 12000 for 10min, then passed through a 0.22um filter membrane; molecular sieve (Sephadex G-50) purification analysis, harvesting activity peak, and freeze-drying; B solution (2% acetonitrile, 0.065% trifluoroacetic acid ) was diluted, centrifuged at 12000g for 10min, and then filtered with a 0.22um filter membrane; the filtrate was reversed for HPLC purification analysis, and the active peak with a retention time of 20.25min was harvested; by solid-phase ch...
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