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High-activity composite antibody oral preparation for resisting bursa fabricius viruses and influenza viruses of chicken

A technology of chicken bursal virus and influenza virus, which is applied in the direction of antiviral agents, antiviral immunoglobulins, antibodies, etc., can solve the problems of non-adherence of pathogenic bacteria, single pathogenic antibody, loss of pathogenicity, etc., and achieves convenient preparation process Practical, low preparation production, and the effect of reducing morbidity

Active Publication Date: 2013-09-25
济南大东农生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention overcomes the disadvantages of the existing antibody preparations that only have a single pathogenic antibody and must be administered by injection, extracts the active ingredient LgY of high-purity poultry yolk antibody through a special process, and mixes it with a high-efficiency stabilizer and a synergist, It can be quickly absorbed after reaching the intestinal tract through oral administration, and can be combined with specific pathogenic bacteria adhesion factors after entering the blood, so that pathogenic bacteria cannot adhere to susceptible cells and lose their pathogenicity

Method used

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  • High-activity composite antibody oral preparation for resisting bursa fabricius viruses and influenza viruses of chicken

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Preparation of chicken infectious bursitis antibody:

[0015] 1. Weigh the bursal tissue with lesions, grind it into a homogenate with a grinder, add sterilized saline at a mass ratio of 1:5, continue to grind evenly, and then filter it with 3 layers of 140-mesh nylon filter, add 0.50% Formaldehyde, mix evenly, place it at 37°C for 24 hours, add 2% Tween 80, mix well, check for miscellaneous bacteria with ordinary medium, store in a cool place after passing the test.

[0016] 2. Preparation of infectious bursitis inactivated oil adjuvant tissue seedlings: select No. 10 white oil and add 3% aluminum hydroxide, mix evenly, heat and fully dissolve. Prepare with the liquid in step 1 at a ratio of 3:1.

[0017] 3. Select healthy high-yield laying hens without specific infectious diseases, inject 1.6ml of infectious bursitis inactivated oil adjuvant tissue vaccine to each chicken subcutaneously.

[0018] 4. After 10-14 days, collect serum from the chicken wing vein t...

Embodiment 2

[0046] Steps (1), (2) are the same as in Example 1.

[0047] (3) Preparation of compound antibody oral preparations:

[0048] 1. Ultrafiltration and concentration: Concentrate the antibody to be used in the above (1) and (2) with an ultrafiltration concentration device with a cut-off value of 80-100KDa under pressure and ultrafiltration to a total protein concentration of 920mg / ml, and the filtrate is Concentrate the antibody and mix it at a volume ratio of 1:1.1.

[0049] 2. Add stabilizer: Add 61g of mannitol and 76g of sucrose powder to 1 liter of purified water to make a clear solution.

[0050] 3. Synergist: Dissolve N-acetylmuramoyl-L-alanyl-D-isoglutamine in purified water to make a 6.5% aqueous solution.

[0051] 4. Mixing: Mix the ingredients in steps 1, 2, and 3 evenly at a volume ratio of 2.1:4.8:0.9.

[0052] 5. Sterilization: The filtrate obtained by ultrafiltration is sterilized by pressure filtration with a filter membrane with a pore size of 0.22 μm.

[005...

Embodiment 3

[0055] Steps (1), (2) are the same as in Example 1.

[0056] (3) Preparation of compound antibody oral preparations:

[0057] 1. Ultrafiltration and concentration: Concentrate the antibody to be used in the above (1) and (2) with ultrafiltration concentration equipment with a cut-off value of 80-100KDa under pressure and ultrafiltration to a total protein concentration of 880mg / ml, and the filtrate is Concentrate the antibody and mix it at a volume ratio of 1:0.9.

[0058] 2. Add stabilizer: Add 59g of mannitol and 74g of sucrose powder to 1 liter of purified water to make a clear solution.

[0059] 3. Synergist: Dissolve N-acetylmuramoyl-L-alanyl-D-isoglutamine in purified water to make a 5.5% aqueous solution.

[0060] 4. Mixing: Mix the ingredients in steps 1, 2, and 3 evenly at a volume ratio of 1.9:5.2:1.1.

[0061] 5. Sterilization: The filtrate obtained by ultrafiltration is sterilized by pressure filtration with a filter membrane with a pore size of 0.22 μm.

[006...

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Abstract

The invention discloses a high-activity composite antibody oral preparation for resisting bursa fabricius viruses and influenza viruses of chicken. The oral preparation is composed of chicken infectious bursa fabricius inflammation concentration antibodies, avian influenza concentration antibodies, stabilizers and synergist. According to the high-activity composite antibody oral preparation, defects that existing antibody preparations are only single pathogene antibodies and have to be dosed by injection are overcome, and high-purity active constituents (LgY) of egg yolk antibodies are extracted by means of a particular process and matched with the efficient stabilizers and the synergist so that the oral preparation is rapidly absorbed after entering intestinal tracts by means of oral dosing and can be combined with specific adherence factors of pathogenic bacteria after entering blood, and the pathogenic bacteria can not adhere sensible cells and lose pathogenicity. The oral preparation is simple in preparation process of the egg yolk antibodies and wide in sources of production materials so that a wide prospect for routinization mass production of the composite egg yolk antibody oral preparation is provided.

Description

technical field [0001] The invention relates to a highly active anti-chicken bursal virus and influenza virus composite antibody oral preparation, which belongs to the technical field of veterinary medicine. Background technique [0002] Yolk antibody is an antibody against a specific antigen extracted from immunized poultry eggs. It is a protein with strong immune function, and its active ingredient is IgY. IgY has good stability in various environments, such as heat resistance, acid resistance, alkali resistance and enzymatic degradation resistance. IgY is tolerant to repeated freezing and thawing. Experiments show that its antigen binding activity is hardly affected after 5 repeated freezing and thawing. Under normal circumstances, IgY can basically withstand pasteurization, and its activity basically does not change significantly at 37°C. When the temperature reaches 65°C, the activity of IgY can be maintained for more than 24h. Adding a suitable adjuvant to the IgY s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/42A61P31/14A61P31/16C07K16/10C07K16/02
Inventor 王立明梁运霞张宝泉李红梅李冬梅车建军
Owner 济南大东农生物技术有限公司
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