High-activity composite antibody oral preparation for resisting bursa fabricius viruses and influenza viruses of chicken
A technology of chicken bursal virus and influenza virus, which is applied in antiviral agents, antiviral immunoglobulins, antibodies, etc., can solve the problems of single pathogenic antibodies, pathogenic bacteria cannot adhere, lose pathogenicity, etc., and achieve low preparation production , The preparation process is convenient and practical, and the effect of reducing the incidence rate
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0014] (1) Preparation of chicken infectious bursitis antibody:
[0015] 1. Weigh the bursal tissue with lesions, grind it into a homogenate with a grinder, add sterilized saline at a mass ratio of 1:5, continue to grind evenly, and then filter it with 3 layers of 140-mesh nylon filter, add 0.50% Formaldehyde, mix evenly, place it at 37°C for 24 hours, add 2% Tween 80, mix well, check for miscellaneous bacteria with ordinary medium, store in a cool place after passing the test.
[0016] 2. Preparation of infectious bursitis inactivated oil adjuvant tissue seedlings: select No. 10 white oil and add 3% aluminum hydroxide, mix evenly, heat and fully dissolve. Prepare with the liquid in step 1 at a ratio of 3:1.
[0017] 3. Select healthy high-yield laying hens without specific infectious diseases, inject 1.6ml of infectious bursitis inactivated oil adjuvant tissue vaccine to each chicken subcutaneously.
[0018] 4. After 10-14 days, collect serum from the chicken wing vein t...
Embodiment 2
[0046] Steps (1), (2) are the same as in Example 1.
[0047] (3) Preparation of compound antibody oral preparations:
[0048] 1. Ultrafiltration and concentration: Concentrate the antibody to be used in the above (1) and (2) with an ultrafiltration concentration device with a cut-off value of 80-100KDa under pressure and ultrafiltration to a total protein concentration of 920mg / ml, and the filtrate is Concentrate the antibody and mix it at a volume ratio of 1:1.1.
[0049] 2. Add stabilizer: Add 61g of mannitol and 76g of sucrose powder to 1 liter of purified water to make a clear solution.
[0050] 3. Synergist: Dissolve N-acetylmuramoyl-L-alanyl-D-isoglutamine in purified water to make a 6.5% aqueous solution.
[0051] 4. Mixing: Mix the ingredients in steps 1, 2, and 3 evenly at a volume ratio of 2.1:4.8:0.9.
[0052] 5. Sterilization: The filtrate obtained by ultrafiltration is sterilized by pressure filtration with a filter membrane with a pore size of 0.22 μm.
[005...
Embodiment 3
[0055] Steps (1), (2) are the same as in Example 1.
[0056] (3) Preparation of compound antibody oral preparations:
[0057] 1. Ultrafiltration and concentration: Concentrate the antibody to be used in the above (1) and (2) with ultrafiltration concentration equipment with a cut-off value of 80-100KDa under pressure and ultrafiltration to a total protein concentration of 880mg / ml, and the filtrate is Concentrate the antibody and mix it at a volume ratio of 1:0.9.
[0058] 2. Add stabilizer: Add 59g of mannitol and 74g of sucrose powder to 1 liter of purified water to make a clear solution.
[0059] 3. Synergist: Dissolve N-acetylmuramoyl-L-alanyl-D-isoglutamine in purified water to make a 5.5% aqueous solution.
[0060] 4. Mixing: Mix the ingredients in steps 1, 2, and 3 evenly at a volume ratio of 1.9:5.2:1.1.
[0061] 5. Sterilization: The filtrate obtained by ultrafiltration is sterilized by pressure filtration with a filter membrane with a pore size of 0.22 μm.
[006...
PUM
Property | Measurement | Unit |
---|---|---|
concentration | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com