Lactobacillus plantarum ZJUF T17 and application thereof
A technology of Lactobacillus plantarum and drugs, applied in the directions of Lactobacillus, application, bacteria, etc., can solve the problem of slightly lower fermentation capacity, and achieve the effects of high survival rate, reduced TNF-α content, and strong gastrointestinal adhesion.
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[0033] 1. Screening and isolation of strains
[0034] Weigh 5g of sourdough and add it to 45mL 0.85% sterilized saline solution, vibrate to make a suspension, carry out serial gradient dilution, take 10% after dilution -5 、10 -6 、10 -7 Three gradients were spread on the MRS medium, and each gradient was made in triplicate. Put the coated plate into an anaerobic incubator at 37°C for 24-48h. The colonies with obvious differences were selected and separated by streaking on the MRS medium, and the plate was placed in an anaerobic incubator at 37°C for 24-48h. After streaking for 3-5 times in a row, take a drop of 5% hydrogen peroxide that is now prepared and drop it on the glass slide, pick a single colony on the solid medium and insert it into the 5% hydrogen peroxide drop and mix evenly to produce Air bubbles are positive for catalase test, no air bubbles are negative for catalase test, and strains with negative catalase test are suspected probiotic strains.
[0035] The s...
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