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Preparation method of silver nuclear mesoporous gold nanostructure material, surface enhanced Raman detecting probe and application thereof

A silver-core mesoporous gold and nanostructure technology, applied in the field of biosensors, can solve problems such as difficult long-term storage, unstable structural properties, complicated operation steps, etc., achieve huge application potential and commercial value, and good surface-enhanced Raman Effects on performance and biocompatibility

Active Publication Date: 2018-09-28
WENZHOU UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the operation steps of these secondary enhancement methods are complicated and the detection time is prolonged, so the optimization of the enhanced substrate is a hot research direction
Since the surface-enhanced Raman scattering effect is related to the surface roughness of SERS nanostructures, a new class of multi-branched nanostructures, such as nanovenus (Schütz, M., Steinigeweg, D., Salehi, M., K., Schlücker, S., 2011, Chem.Commun., 47:4216.), nano-gold sea urchin (Liu, Z., Yang, Z., Peng, B., Cao, C., Zhang, C., You, H., Xiong, Q., Li, Z., Fang, J., 2014, Adv. Mater., 26:2431-2439.) and nano gold flowers (Jiang, Y., Wu, X.J., Li, Q., Li, J., Xu, D., 2011, Nanotechnology, 22:385601.), its surface roughness has a great advantage compared with spherical particles of the same size, and the extremely small radius of curvature in such nanoparticles Lead to strong electric field enhancement, but its structural properties are unstable, and it is not easy to store for a long time

Method used

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  • Preparation method of silver nuclear mesoporous gold nanostructure material, surface enhanced Raman detecting probe and application thereof
  • Preparation method of silver nuclear mesoporous gold nanostructure material, surface enhanced Raman detecting probe and application thereof
  • Preparation method of silver nuclear mesoporous gold nanostructure material, surface enhanced Raman detecting probe and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: Preparation of 20nm silver nano sol

[0043] S1: 1 mL of 1% trisodium citrate and 30 μL of 0.1 M sodium chloride solution were sequentially added to 2.0 mL of 0.01 M silver nitrate solution, and then mixed.

[0044] S2: After 5 min, the mixture was quickly added to 50 mL of boiling water containing 100 μL of 1% ascorbic acid, and ascorbic acid was added 1 min before adding the mixture.

[0045] S3: Keep the solution boiling for 1h, then cool to room temperature under stirring to obtain a silver nanoparticle sol.

[0046] S4: Centrifuge the obtained silver nanoparticle sol and redisperse the precipitate in water to obtain a silver nanosol of about 20nm, which is named as M1.

Embodiment 2-3

[0047] Embodiment 2-3: 50nm and 60nm silver nano-sol preparation

[0048] Except that the amount of silver nitrate in step S1 is replaced by 2.4mL and 2.7mL, other operations remain unchanged, thereby repeating Example 1, thereby carrying out Example 2-3 in sequence, and the gained 50nm and 60nm silver nano-sol Named M2 and M3 in turn.

Embodiment 4

[0049] Example 4: Preparation of 50nm silver core mesoporous gold

[0050] S1: At room temperature, 6 mL of 50 nm silver nanoparticle hydrosol was added into a glass bottle and sonicated for 30 min.

[0051] S2: After 30min, use a peristaltic pump to add different volumes (0.5mL, 1.0mL, 1.5mL, 2.0mL) of 0.25mM HAuCl 4 the aqueous solution,

[0052] S3: The mixture was continuously stirred for 30 min, and silver-core porous gold sols with different gold-silver ratios were finally prepared, named M2-Y1, M2-Y2, M2-Y3, and M2-Y4 respectively.

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Abstract

The invention discloses a preparation method of a silver nuclear mesoporous gold nanostructure material, a surface enhanced Raman detecting probe and application thereof. Firstly, silver nuclear mesoporous gold with different sizes and different gold-silver ratios is prepared; then, the enhancing effect is detected; the silver nuclear mesoporous gold is compared with gold and silver nanoparticleswith the same size to obtain the silver nuclear mesoporous gold with optical conditions; and the silver nuclear mesoporous gold with the optical conditions is biologically functionalized to apply to the field of Raman biosensors. The method designs a novel gold-silver proportional nuclear shell Raman enhanced nanostructure; compared with such nanostructures as general nanogold, nanometer golden flowers and nanosilver, the novel gold-silver proportional nuclear shell Raman enhanced nanostructure has the characteristics of high Raman enhancing activity, excellent biocompatibility and the like; and a surface enhanced Raman detecting probe built by the novel gold-silver proportional nuclear shell Raman enhanced nanostructure achieves more important significance on the aspects of environmentalmonitoring, food safety, clinical diagnosis and the like.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and specifically refers to a preparation method of a silver-core mesoporous gold nanostructure material, a surface-enhanced Raman detection probe and an application thereof in biosensors. Background technique [0002] A variety of new biosensor technologies have been developed, including electrochemical, fluorescence and surface plasmon resonance. These existing biosensor technologies have some disadvantages, such as tedious sample preparation, large sample volume requirement, limited multiplexing and specificity, and high detection cost. Therefore, there is an urgent need to develop simple, highly specific, and inexpensive methods to detect cancer biomarker levels in normal and cancer patient sera. [0003] Surface-enhanced Raman scattering spectroscopy based on the localized surface plasmon resonance effect of gold and silver nanostructures has been developed as a powerful and nondestructiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24B22F1/00B22F1/02G01N21/65B82Y40/00
CPCG01N21/658B82Y40/00B22F9/24B22F1/054B22F1/17
Inventor 林大杰黄亚齐李梦婷王舜金辉乐
Owner WENZHOU UNIVERSITY
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