Method of degrading spirulina
A technology of spirulina and degrading bacteria, applied in the field of water treatment, can solve the problems of only inhibition rate and large input amount, and achieve the effect of improving capacity, less input amount and high removal rate
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[0037] The preparation method of described untreated bacterial liquid comprises:
[0038] (1) Get the Pseudomonas mendoza preserved in the refrigerator, draw a line on the nutrient broth solid medium plate, and culture it at a temperature of 26-33°C for 10-16h until a single colony grows;
[0039] (2) Inoculate a single colony of Pseudomonas mendoza into the nutrient broth liquid medium, and cultivate it for 10-16h to the logarithmic growth phase under the condition that the temperature is 26-33°C and the number of revolutions is 150-250rpm, and then press 0.5-2% of the added amount was transferred to fresh nutrient broth liquid medium, and continued to cultivate to the logarithmic growth phase according to the above conditions to obtain untreated bacterial liquid;
[0040] (3) centrifuging the untreated bacterial liquid, collecting the centrifuged bacterial cells, and resuspending them with sterile water to obtain bacterial supersuspensions;
[0041] (4) Collect the supernat...
Embodiment 1
[0075] 1. Preparation of degradation bacteria solution
[0076] Take the Pseudomonas mendoza preserved in the refrigerator at -80°C, streak it on the nutrient broth solid medium plate, and culture it at 30°C for 12 hours until a single colony grows; inoculate Pseudomonas mendoza Bacterial single colonies were transferred to the nutrient broth liquid medium, cultivated at a temperature of 30°C and a rotation speed of 200rpm for 16 hours to the logarithmic growth phase, and then transferred to a fresh nutrient broth liquid medium according to the addition amount of 1%. Cultivate for 12 hours to the logarithmic growth phase at a temperature of 30° C. and a rotation speed of 200 rpm to obtain an untreated bacterial liquid for future use.
[0077] 2. Prepare the culture medium of Spirulina
[0078] Spirulina was inoculated in BG11 medium with 10% addition amount, and cultured for 3 days at a temperature of 30°C and a light intensity of 2000 lux. The measured chlorophyll a content ...
Embodiment 2
[0083] 1. Preparation of degradation bacteria solution
[0084] Take the Pseudomonas mendoza preserved in the refrigerator at -80°C, streak it on the nutrient broth solid medium plate, and culture it at 30°C for 12 hours until a single colony grows; inoculate Pseudomonas mendoza Bacterial single colonies were transferred to the nutrient broth liquid medium, cultivated at a temperature of 30°C and a rotation speed of 200rpm for 16 hours to the logarithmic growth phase, and then transferred to a fresh nutrient broth liquid medium according to the addition amount of 1%. Cultivate at a temperature of 30°C and a rotation speed of 200 rpm for 16 hours to the logarithmic growth phase to obtain an untreated bacterial liquid for later use;
[0085] Centrifuge the above-mentioned untreated bacterial liquid at a speed of 10,000 rpm and a temperature of 4°C. After collecting the bacterial cells, resuspend them with sterile water to obtain a bacterial supersuspension;
[0086] The above c...
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