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Application of angelica protein to preparation of medicine for assisting tumor therapy

A tumor treatment and protein technology, applied in anti-tumor drugs, drug combinations, peptide/protein components, etc., to increase the quantity, improve the quality of life, and enhance the vitality

Active Publication Date: 2018-10-02
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the course of clinical use, amifostine has gradually been found to have greater toxicity, causing most patients to experience adverse reactions such as dizziness, nausea, and vomiting.

Method used

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  • Application of angelica protein to preparation of medicine for assisting tumor therapy
  • Application of angelica protein to preparation of medicine for assisting tumor therapy
  • Application of angelica protein to preparation of medicine for assisting tumor therapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Purification of Angelica ASPR protein

[0036] Soak the cut Angelica sinensis in 10 times the volume of 0.05 mol / L Tris-HCl buffer (pH 8.0), and let it stand overnight at 4°C for about 15 hours. The residue was filtered with 4 layers of gauze the next day, and the filtrate was heated at 12000 rpm, 4 Centrifuge at ℃ for 10 min, the supernatant obtained is the crude angelica protein extract; the crude protein solution is subjected to ammonium sulfate one-step precipitation (0-80%), and the precipitated protein is collected overnight and dissolved in 2 times the volume of 0.05 mol / L Tris -In HCl buffer (pH 8.0), load the dissolved solution of ammonium sulfate precipitation on the Sephadex G-50 column after fully equilibrated with the balance solution (0.05 mol / L, pH 8.0 Tris-HCl buffer), Elute with equilibrium solution and collect the second elution peak, and detect the purity by SDS-PAGE electrophoresis.

[0037] Experimental results:

[0038] After crude extraction ...

Embodiment 2

[0039] Example 2: Protective effect of Angelica ASPR protein on mice injured by cisplatin

[0040] 1. Laboratory animals and grouping

[0041] Male Kunming mice, purchased from Wu's animals, weighing 20 ± 2 g. They were fed normally for three days before the experiment. The mice were randomly divided into 5 groups, each with 5 mice. Each group and its treatment were as follows: the normal group (CON) and the cisplatin injury group (DDP) were injected with 0.3 mL of normal saline for 3 days, and 2 hours after the last administration, The CON group was injected with 0.3 mL of normal saline, and the DDP group was injected with 0.3 mL of 0.5 mg / mL cisplatin solution (7.5 mg / kg); the protein addition group was ASPR1 (0.5 mg / mL) + DDP group, ASPR2 (1.25 mg / mL) )+DDP group and ASPR3 (2.0 mg / mL)+DDP group, the three groups of mice were injected with 0.5 mL of protein solution for 3 days. Two hours after the last administration, the mice were injected with 0.3 mL of cisplatin solution.

[...

Embodiment 3

[0048] Example 3: Preventive effect on radiation protection of mice with one-time whole body radiation

[0049] 1. Experimental animals

[0050] Male Kunming mice, purchased from Wu's animals, weighing 20 ± 2 g. Drink water and eat freely, the feed is specially irradiated and exhausted regularly. There was no abnormality after feeding for three days before the experiment.

[0051] 2. Irradiation conditions and experimental grouping

[0052] The experimental Kunming mice were randomly divided into 5 groups: the normal group CON (no dose and no radiation), the radiation control group XRT (only radiation and no dose), and the amifostine group (AMFT+XRT) (200 mg / kg) , 30 min before radiation), Angelica ASPR protein group (ASPR+XRT) (1.25 mg / mL), the pre-radiation administration group was intraperitoneally injected with Angelica ASPR protein for 3 days, and radiation was performed 2 hours after the last injection. The radiation source is X-ray, the radiation dose is 6 Gy, the dose rate ...

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Abstract

The invention relates to the field of natural Chinese herbal protein, in particular to application of angelica protein to preparation of a medicine for assisting tumor therapy. The N-terminal sequenceof the angelica protein is GIQKTEVEAPSTVSA. The angelica protein is applied to preparation of the medicine for assisting the tumor therapy; through administration of the angelica protein before chemotherapy, damage of a chemotherapeutic medicine cis-platinum to the spleen of a mouse can be reduced; through administration of the angelica protein before radiotherapy, the number of peripheral bloodleukocytes, thymus indexes, spleen indexes, the number of spleen nodules (CFU-S) and the spleen CAT activity of an irradiated mouse can be significantly increased by 135%, 103%, 23%, 219% and 180% respectively, the GST and GSH-Px activity of the spleen of the irradiated mouse can be also improved, and white pulp damage of the spleen is improved; the angelica protein has an overall protective effect stronger than that of amifostine, can significantly reduce the weight loss rate of the irradiated mouse by 94%, and thus improves the survival quality thereof.

Description

Technical field [0001] The present invention relates to the field of natural Chinese medicine protein, and more specifically to the application of angelica protein in the preparation of auxiliary tumor treatment drugs. Background technique [0002] The chemotherapeutic drug cisplatin (DDP) is one of platinum-based broad-spectrum anticancer drugs with clinical activity. However, due to its low selectivity, it has a strong killing effect on tumor cells and can also inhibit the spleen Represents the immune system. [0003] Radiotherapy is an effective means to kill tumors, and it is responsible for the treatment of 2 / 3 tumor patients. However, the side effects of radiation damage caused by free radicals produced by radiotherapy are severe, which severely weakens the body's local and overall resistance. Patients are often forced to interrupt radiotherapy because of these side effects. Therefore, the prevention and treatment of side effects of tumor radiotherapy will directly affect w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/10A61P35/00A61P1/14A61K33/24
CPCA61K33/24A61K38/10A61P1/14A61P35/00A61K2300/00
Inventor 潘剑茹王香玲
Owner FUZHOU UNIV
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