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Sensitized cytochrome c optical fiber sensing device and method

A cytochrome, optical fiber sensing technology, applied in the field of biomedical optical sensing technology design, can solve the problems of low detection limit, expensive, insufficient to track apoptosis in situ, etc., to enhance the sensitivity of evanescent wave and optical fiber sensing Enhanced effect

Active Publication Date: 2021-02-05
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The lowest limit of detection (LOD) in most of these methods is only 10 -12 M, is not enough to track the occurrence of apoptosis in situ, and requires expensive, large-scale instruments and complicated operations. Therefore, the development of cytochrome c molecular sensors with simple operation, low cost, high sensitivity and low detection limit plays an important role

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  • Sensitized cytochrome c optical fiber sensing device and method
  • Sensitized cytochrome c optical fiber sensing device and method
  • Sensitized cytochrome c optical fiber sensing device and method

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Embodiment 1

[0039]Optical fiber sensing technology uses 100-micron-scale optical fiber physical media and light waves as information carriers. It has the advantages of low cost, small structure, high sensitivity, remote monitoring, corrosion resistance, and strong biological compatibility. It has become the fastest growing in recent years. One of the most biosensing technologies. In the related reports of optical fiber biosensing research, high-performance optical fiber interferometer has become a research hotspot. The most representative one is the tapered micro-nano fiber interferometer sensor developed in recent years. In addition to the characteristics of conventional fiber sensors, this type of fiber sensor can also use the evanescent wave mode that is sensitive to the surrounding environment. , Not only greatly enriches its detection objects, but also improves measurement accuracy. It has very broad application prospects in the field of biomedical testing.

[0040]Such asfigure 1 As shown, t...

Embodiment 2

[0044]Such asfigure 1 As shown, this embodiment provides a sensitized cytochrome c optical fiber sensing method. The method includes: drawing a photosensitive optical fiber on a flame into a tapered micro-nano fiber 1 with a diameter of 10.5 micrometers. Fiber 1 is fusion spliced ​​with a single-mode fiber to make a fiber-optic sensing probe; the graphene interface 2 modified with silver nanoparticles is assembled on the side of the tapered micro-nano fiber 1 to form a single-layer film, and the DNA is conformed to by non-covalent bonding. The ligand 3 is fixed on the surface of the graphene interface 2; after the DNA aptamer 3 is fixed, the optical fiber sensing probe is immersed in the culture medium containing the cells, and the light source 4 is input into the tapered micro-nano fiber, using the cone The evanescent wave on the side of the micro-nano fiber 1 is sensitive to changes in the external environment, and detects the conformational changes of DNA aptamer 3 caused by the ...

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Abstract

The invention discloses a sensitized cytochrome c optical fiber sensing device and method. The device comprises a tapered micro-nano fiber, a graphene interface and a DNA aptamer; the transition zone and the uniform zone of the tapered micro-nano fiber form interference structure, and the tapered micro-nano fiber and single-mode fiber are fused to form a fiber sensing probe; the graphene interface is a graphene interface modified by silver nanoparticles, and the graphene interface is assembled into a single-layer film on the side of the tapered micro-nano fiber ; DNA aptamers are immobilized on the surface of the graphene interface through non-covalent bonding; the optical fiber sensing probe is immersed in the culture medium containing cells after the DNA aptamers are immobilized, and the light source is input into the tapered micro-nano optical fiber , using the characteristic that the evanescent wave on the side of the tapered micro-nano fiber is sensitive to changes in the external environment, the conformational change of the aptamer caused by the specific binding of the cytochrome c molecule to the DNA aptamer was detected. The invention can realize extracellular and in-situ measurement of cytochrome c in the human body, avoiding invasive damage and toxicity to cells.

Description

Technical field[0001]The invention relates to an optical fiber sensing device, in particular to a sensitization type cytochrome c optical fiber sensing device and method, belonging to the field of biomedical optical sensing technology design.Background technique[0002]Cytochrome c, as a multifunctional enzyme, is an important electron transporter. In the process of cell apoptosis, cytochrome c is released from mitochondria and then released from the cell when the cell ruptures. Its release is an important signal of programmed cell death-apoptosis. In clinical disease diagnosis, once pathological conditions are caused by heart failure, DNA damage, cytoskeleton destruction, or foreign cell invasion, cytochrome c in mitochondria can be released into the blood by damaging the permeability of mitochondria. Therefore, the monitoring of cytochrome c not only provides biomarkers for apoptosis, but also has important significance for understanding certain diseases at the cell level.[0003]The ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/45
CPCG01N21/45G01N2021/458
Inventor 黄赟赟李宏韬关柏鸥
Owner JINAN UNIVERSITY