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Refining method of pumpkin oligosaccharides by macroporous resin

A technology of macroporous resin and refining method, which is applied in the preparation of oligosaccharides and sugar derivatives, chemical instruments and methods, etc., can solve the problems of low removal rate of impurities, poor decolorization effect of colored substances, and destruction of oligosaccharide structure, etc. Achieve high purity, less waste, and low energy consumption

Active Publication Date: 2020-12-15
国农绿源(北京)农业科技研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for refining pumpkin oligosaccharides by using macroporous resin, so as to overcome the shortcomings of poor decolorization effect of colored substances in existing pumpkin oligosaccharides, low removal rate of impurities, and destruction of oligosaccharide structure

Method used

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  • Refining method of pumpkin oligosaccharides by macroporous resin
  • Refining method of pumpkin oligosaccharides by macroporous resin
  • Refining method of pumpkin oligosaccharides by macroporous resin

Examples

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Effect test

Embodiment 1

[0049] A method for purifying pumpkin oligosaccharides by using macroporous resin, which is carried out according to the following steps:

[0050] (1) Macroporous resin pretreatment: soak DM28 macroporous adsorption resin in deionized water for 24 hours, wash with ethanol until it is not turbid, soak in ethanol for 24 hours, wash with water until there is no alcohol smell, and use NaOH solution with a concentration of 5% by mass Pass through the resin column and soak for 2 hours, wash with water until neutral, pass HCl with a concentration of 5% by mass through the resin column and soak for 4 hours, wash with water until neutral, and set aside at 4 °C; put it into a chromatography column (6cm×80cm)3 / 4 places to ensure that the filling is even and flat and no air bubbles;

[0051] (2) Add water to the crude extract of pumpkin oligosaccharide to make a solution with a concentration of 250 mg / ml, and pass the above solution of 1.0 times the column volume (BV) through a chromatog...

Embodiment 2

[0063] A method for purifying pumpkin oligosaccharides by using macroporous resin, which is carried out according to the following steps:

[0064] (1) Macroporous resin pretreatment: soak DM21 macroporous adsorption resin in deionized water for 30h, wash with ethanol until it is not cloudy, soak in ethanol for 30h, wash with water until there is no alcohol smell, use NaOH solution with a concentration of 5% by mass Pass through the resin column and soak for 4 hours, wash with water until neutral, pass HCl with a concentration of 5% by mass through the resin column and soak for 4 hours, wash with water until neutral, and set aside at 4 °C; The filling is uniform and flat without bubbles;

[0065] (2) Add water to the crude extract of pumpkin oligosaccharide to form a solution with a concentration of 280 mg / ml, and pass the above solution of 1.5 times the column volume (BV) through a chromatographic column equipped with a macroporous resin at a flow rate of 1.5 BV / h , carry out...

Embodiment 3

[0075] The pumpkin oligosaccharide crude extract used in this example is the pumpkin oligosaccharide crude extract prepared in Example 1.

[0076] A method for purifying pumpkin oligosaccharides by using macroporous resin, which is carried out according to the following steps:

[0077] (1) Macroporous resin pretreatment: soak DM28 macroporous adsorption resin in deionized water for 20 hours, wash with ethanol until it is not turbid, soak in ethanol for 20 hours, wash with water until there is no alcohol smell, and use NaOH solution with a concentration of 5% by mass Pass through the resin column and soak for 1 h, wash with water until neutral, pass HCl with a concentration of 5% by mass through the resin column and soak for 1 h, wash with water until neutral, and set aside at 4 °C; load it into a chromatography column (6cm×80cm)3 / 4 places to ensure that the filling is even and flat and no air bubbles;

[0078] (2) Add water to the crude extract of pumpkin oligosaccharide to ...

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Abstract

The invention discloses a method for refining pumpkin oligosaccharides by using macroporous resin. The method comprises the following steps: adding water to a pumpkin oligosaccharide crude extract toprepare a pumpkin oligosaccharide column-loading solution, performing absorption by chromatography columns loaded with macroporous resin and collecting effluent liquid; performing standing, rinsing the macroporous resin with deionized water, and performing concentration by evaporation and freeze-drying to obtain colorless pumpkin oligosaccharides; performing washing with an ethanol solution and soaking with ethanol, allowing an NaOH solution to pass through and soak the resin columns, and performing water washing to neutral; allowing hydrochloric acid to pass through and soak the resin columns, and recovering the macroporous resin; and performing rotary evaporation and freeze drying, and recovering colored impurities and the ethanol. By the refining method, high-efficiency discoloration ofthe pumpkin oligosaccharides is achieved while monosaccharides and protein impurities are simultaneously removed, the purity of the obtained pumpkin oligosaccharides is as high as 95-96%, also the process has mild treatment conditions, does not adopt high-temperature or acid and alkali treatment and cannot cause degradation of the pumpkin oligosaccharides, and the obtained colored impurity melanoidin can be recovered and utilized.

Description

technical field [0001] The invention belongs to the technical field of purification of pumpkin oligosaccharides, in particular to a method for purifying pumpkin oligosaccharides by using macroporous resin. Background technique [0002] A large number of studies have confirmed that functional sugar components such as polysaccharides and oligosaccharides in pumpkin have the function of regulating blood sugar, and the functional sugar, as a natural plant-derived component, has also been widely recognized for its safety. Oligosaccharides, also known as oligosaccharides, are oligosaccharides composed of 2-10 monosaccharides connected by glycosidic bonds into straight or branched chains, with molecular weights between 200 and 3000D. Due to the different binding positions and binding types of monosaccharide molecules, a rich variety of oligosaccharides are formed. Oligosaccharides have good functional properties and can be used as a source of sweetness, but they are not degraded b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/06C07H3/04C07H3/06C08B37/00C09B61/00A23L5/43A23L33/10A61P39/06A61P3/10A61P1/02
CPCA23V2002/00A23L5/43A23L33/10A61P1/02A61P3/10A61P39/06C07H1/06C07H3/04C07H3/06C08B37/003C09B61/00A23V2200/02A23V2200/04A23V2200/312A23V2200/328
Inventor 李全宏梁莉
Owner 国农绿源(北京)农业科技研究院有限公司
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