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Purification method of Italian toothedfruit dock leaf total flavone

A purification method and a technology of Phytophthora vulgaris, which are applied in the field of separation and purification, can solve the problems such as the content of total flavonoid products needs to be improved, and achieve the effect of simple and convenient purification process.

Inactive Publication Date: 2018-10-23
XINJIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there are few studies on the purification process of the total flavonoids of Buglossia flavonoids. Chinese patent CN101584752B discloses an extraction and purification process of total flavonoids of Buglossia flavonoids. This patented technology uses macroporous adsorption resin as a purification medium to enrich flavonoids , the total flavonoid content of Buglossia in the purified product>55%, the total flavonoid product content needs to be improved

Method used

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  • Purification method of Italian toothedfruit dock leaf total flavone
  • Purification method of Italian toothedfruit dock leaf total flavone
  • Purification method of Italian toothedfruit dock leaf total flavone

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preparation example Construction

[0029] In the present invention, the preparation method of the crude extract of total flavonoids of Buglossia preferably comprises the following steps: using an ethanol solution with a volume fraction of 70-80% to extract Buglossia italiana to obtain crude total flavonoids of Buglossia Extraction. The present invention specifically takes the dry aboveground part of Buglossia italiana, removes impurities such as sand and stones, pulverizes through a 20-mesh sieve, and adds the obtained Buglossia powder into an ethanol solution with a volume fraction of 70-80%. Reflux extraction for 1.5-2.5 hours at a temperature of 1:15-25 at a temperature of 90-100°C, and the extracted product was sequentially filtered, concentrated under reduced pressure, and passed through a 40-mesh sieve to obtain the crude extract of total flavonoids of Buglossia italiana Powder: mix the crude extract powder of total flavonoids of Buglossia italiana with distilled water, dissolve it by ultrasonic, centrifu...

Embodiment 1

[0046] Get the dry aerial parts of Buglossia militaris, remove impurities such as sand and stones, crush through a 20-mesh sieve, weigh 100 g of Buglossia glabra powder obtained, add 20 times the amount of ethanol with a volume fraction of 75%, and place in a water bath at 100 ° C. Mid-reflux extraction twice, 2 hours each time, combined extracts, double-layer gauze to filter off medicinal residues, concentrated under reduced pressure, and dried in a vacuum oven at 70°C to constant weight to obtain the crude extract of total flavonoids of Buglossia flavonoids Powder: Take 20g of the crude extract powder of Buglossia italiana total flavonoids, place it in a 1000mL volumetric flask, dilute it to the mark with distilled water, dissolve it with ultrasonic waves, centrifuge at 5000r / min for 10min, take the supernatant, and obtain the crude extract of Buglossia italiana substance solution (20mg·mL ﹣1 );

[0047] Soak the polyamide in 95% ethanol for 24 hours, wet-pack the column, e...

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Abstract

The invention provides a purification method of Italian toothedfruit dock leaf total flavone. The Italian toothedfruit dock leaf total flavone is purified and enriched by a polyamide column chromatography, the total flavone content in acquired purified substances can reach 75%, and the total flavone content in effective parts of the Italian toothedfruit dock leaf total flavone is remarkably improved. Five effective components such as 1-caffeic acid, 2-rutin, 3-kaempferol-3-O-rutinoside, 4-narcissoside and 5-rosmarinic acid are separated from the effective parts of the Italian toothedfruit dockleaf total flavone, so that the effective components of the Italian toothedfruit dock leaf total flavone are remained when the Italian toothedfruit dock leaf total flavone is purified and enriched bythe polyamide column chromatography, and an experimental foundation is laid for development and research of the effective parts of the Italian toothedfruit dock leaf total flavone.

Description

technical field [0001] The invention relates to the technical field of separation and purification, in particular to a method for purifying total flavonoids of Buglossia flavonoids. Background technique [0002] Flavonoids are plant secondary metabolites, which have various pharmacological effects such as anti-oxidation, anti-inflammation, anti-tumor and immune enhancement due to their complex and diverse structural types. And a large number of literatures show that oral flavonoids have a certain therapeutic effect on myocardial ischemia-reperfusion injury. [0003] Italian Bugloss (Anchusaitalica Retz.) is a herbaceous plant of Boraginaceae (Boraginaceae), which is distributed in the Mediterranean and tropical regions. It is one of the first choice medicinal materials for cardiovascular and cerebrovascular diseases commonly used in Uighur medicine. Uighur medical theory believes that Italian Buglossia has the functions of generating dampness and heat, regulating abnormal ...

Claims

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Application Information

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IPC IPC(8): A61K36/30
CPCA61K36/30A61K2236/55
Inventor 马桂芝滕亮路文杰郭伟
Owner XINJIANG MEDICAL UNIV