Preparation method for easy-suturing high-simulation tissue engineering neural repair scaffold

A technology for simulating tissue and nerve repair, applied in the field of nerve repair tissue engineering, can solve the problems of porous scaffolds with flat ends that are not easy to be sutured, easy to collapse, and lack of internal three-dimensional structure, so as to guide nerve tissue regeneration, relieve pain, and solve problems such as lack of The effect of the internal three-dimensional structure

Inactive Publication Date: 2018-10-30
沈阳尚贤再生医学科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the technical problems existing in the known technology, the present invention provides a preparation method of an easy-to-suture high-simulation tissue engineering neural scaffold, which solves the problem that the previous scaffold products lack internal three-dimensional structure and is easy to collapse, and at the same time solves the problem that the flat end of the porous scaffold is not easy to suture The problem

Method used

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  • Preparation method for easy-suturing high-simulation tissue engineering neural repair scaffold
  • Preparation method for easy-suturing high-simulation tissue engineering neural repair scaffold
  • Preparation method for easy-suturing high-simulation tissue engineering neural repair scaffold

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preparation example Construction

[0027] The easy-to-suture highly simulated tissue engineering nerve repair scaffold and the preparation method thereof of the present invention include the steps of preparing a suspension, preparing an inner core of an array microtube, preparing a collagen skin, and cutting and assembling. in,

[0028] The step of preparing the suspension comprises: taking collagen and chitosan, mixing them and dissolving them in acetic acid solution to obtain a collagen-chitosan suspension; in this step, the collagen and chitosan The weight ratio between them is 1:0~5; the mass concentration of the acetic acid solution is 3~8mg / mL, and the weight ratio of the weight sum of collagen and chitosan to the acetic acid solution is 1~10:200. After dissolving collagen and chitosan raw materials in acetic acid solution, use a homogenizer to intermittently stir in an ice-water bath, and the stirring speed is controlled at 2500-3500rpm; after mixing, use a centrifuge to centrifuge at 10,000rpm for 30min...

Embodiment 1

[0036] A method for preparing an easy-to-suture highly simulated tissue engineered nerve repair scaffold, which is carried out according to the following steps:

[0037] (1) Suspension preparation: Weigh 200 mg of collagen and dissolve it in 4 g of acetic acid solution with a mass concentration of 6 mg / mL, stir intermittently in an ice-water bath with a homogenizer, control the stirring speed at 2500-3500 rpm, and mix thoroughly. After uniformity, use a centrifuge to centrifuge at a speed of 10000rpm for 30min to remove air bubbles;

[0038] (2) Preparation of array microtube inner core: inject the suspension after removing air bubbles into one end of the inner core mold, prevent the generation of air bubbles during the injection process, and then plug the injection end of the inner core mold to match the inner diameter of the inner mold shell. The temperature-conducting rod, and then connect multiple injected inner core molds to the uniform falling device; with 2×10 -5 Immer...

Embodiment 2

[0042] The difference with Example 1 is: when the suspension is prepared, take 280mg collagen and 70mg chitosan, mix and dissolve in 28g acetic acid solution of 5mg / mL and stir for processing; when the array microtube inner core is prepared, 1×10 after injection molding -6 Gradient cold showering at a speed of m / s, and then immerse the lyophilized array microtube inner core in 1% genipin solution for cross-linking for 12 hours; when preparing the scaffold skin, immerse it in distilled water with a pH of 8 after injection molding, After drying, soak in 1% genipin and cross-link for 24 hours; when preparing the easy-to-suture highly simulated tissue engineered nerve scaffold, insert the scaffold inner core 7 into the scaffold outer skin 8 and leave a 3 mm outer skin length at both ends.

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Abstract

The invention relates to a preparation method for an easy-suturing high-simulation tissue engineering neural repair scaffold. The preparation method comprises a step of preparing suspension, a step ofpreparing an array micro-pipe inner core, a step of preparing collagen outer skin and a step of cutting and assembling. The step of preparing the suspension comprises the following steps: weighing collagen and chitosan, after uniformly mixing, and dissolving in acetic acid solution. The step of preparing the array micro-pipe inner core comprises the following steps: enabling the suspension to beinjected in a mould, inserting a temperature conducting rod, immerging in liquid nitrogen and performing gradient cooling, freeze-drying, crosslinking, washing, freeze-storing and freeze-drying again.The step of preparing the collagen outer skin comprises the following steps: enabling the suspension to be injected in the mould, washing, drying, crosslinking, washing again, freeze-storing and drying again. The step of cutting and assembling comprises the following steps: cutting and assembling. The provided rational preparation method is capable of solving problems of an existing scaffold product that an internal three-dimensional structure is lacked and the collapse is easy, and solving a problem that the parallel and level tail end of the porous scaffold is not easily sutured.

Description

technical field [0001] The invention belongs to the technical field of nerve repair tissue engineering, and in particular relates to a preparation method of an easy-to-suture highly simulated tissue engineering nerve repair bracket. Background technique [0002] Peripheral nerve injury, especially peripheral nerve defect, is the most serious type of trauma clinically, which often leads to sensory and motor dysfunction or loss in patients, seriously affecting patients' daily life and work. For short-segment nerve injuries, the severed nerve can be anastomosed directly to the end, so that the proximal regenerated nerve fibers can grow into the distal end. However, for long-segment nerve defects, tension-free suture cannot be performed clinically. For a long time, free autologous nerve transplantation has been used to repair peripheral nerve defects, but it will suffer from insufficient nerve sources at the donor site, secondary nerve function loss at the donor site, and donor-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/50A61L27/56A61L27/58
CPCA61L27/26A61L27/50A61L27/56A61L27/58A61L2430/32C08L5/08C08L89/00
Inventor 蔡盼盼李瑞欣李洪谊陈玉龙孙明志孙杨王耀涓刘斌高健
Owner 沈阳尚贤再生医学科技股份有限公司
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