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Deep-sea fish skin collagen peptide

A deep-sea fish skin and collagen technology, applied in animal/human proteins, specific peptides, connective tissue peptides, etc., can solve the problems of unsatisfactory adsorption effect of composite activated carbon, and achieve good application value, market potential, and sensory acceptance. High, biologically active effect

Inactive Publication Date: 2018-11-02
金华市铁骑士生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the adsorption effect of the composite activated carbon used in this preparation method is not ideal.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for preparing deep-sea fish skin collagen peptides, adding deionized water containing potassium chloride and sodium carboxymethylcellulose to the deep-sea fish skin collagen, enzymatically hydrolyzing the deep-sea fish skin, decolorizing, filtering, and Deep-sea fish skin collagen peptide was obtained by freeze-drying. The preparation method has the advantages of low investment, simple operation, good protein degradation effect, high yield of fish skin glue protein peptide, whiter collagen peptide, higher sensory acceptance, easy industrial scale production, and has good application value and market potential.

[0021] The specific steps of enzymatic hydrolysis are as follows: add deionized water to the deep-sea fish skin collagen according to the substrate concentration of 8%, adjust the pH to 8.6, add the compound protease containing collagenase and flavor protease according to the enzyme amount of 4%, and then Enzymolysis was carried out at 40°C for 10 hour...

Embodiment 2

[0030] A preparation method of deep-sea fish skin collagen peptide, the specific steps are:

[0031] 1) Enzymatic hydrolysis: add deionized water to the deep-sea fish skin collagen according to the substrate concentration of 9%, adjust the pH to 8.0, add the compound protease containing collagenase and flavor protease according to the enzyme amount of 4.8%, and then add the protease at temperature Enzymolysis at 35°C for 12 hours to obtain the enzymolysis liquid, the weight ratio of collagenase and flavor protease in the above composite protease is 1:0.6, deionized water contains 0.3M potassium chloride and 4mM sodium carboxymethylcellulose;

[0032] 2) Decolorization: raise the temperature of the enzymolysis solution to 60°C, adjust the pH to 6, add modified activated carbon with 2% collagen weight, stir ultrasonically for 15 minutes, and filter to obtain the clear filtrate. The preparation method of the above modified activated carbon is: according to the material The liquid...

Embodiment 3

[0035] A preparation method of deep-sea fish skin collagen peptide, the specific steps are:

[0036] 1) Enzymatic hydrolysis: add deionized water to the deep-sea fish skin collagen according to the substrate concentration of 10%, adjust the pH to 7.4, add a compound protease containing collagenase and flavor protease according to the enzyme amount of 5.5%, and then Enzymolysis at 30°C for 15 hours to obtain the enzymolysis solution, the weight ratio of collagenase and flavor protease in the above composite protease is 1:0.45, deionized water contains 0.4M potassium chloride and 3mM sodium carboxymethylcellulose;

[0037] 2) Decolorization: raise the temperature of the enzymolysis solution to 70°C, adjust the pH to 5, add modified activated carbon with 3% collagen weight, stir ultrasonically for 10 minutes, and filter to obtain the clear filtrate. The preparation method of the above modified activated carbon is: according to the material The liquid ratio is 1:12g / mL, soak the a...

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PUM

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Abstract

The invention discloses a deep-sea fish skin collagen peptide. The preparation method of the deep-sea fish skin collagen peptide comprises the following steps: adding deionized water containing potassium chloride and sodium carboxymethylcellulose into deep-sea fish skin collagens, performing enzymolysis with compound protease, discoloring and filtering enzymatic hydrolysate, and performing freezedrying, thereby obtaining the deep-sea fish skin collagen peptide. The deep-sea fish skin collagen peptide disclosed by the invention has the beneficial effects that the collagen peptide is relativelywhite, high in sensory acceptance and high in biological activity and can be widely applied to the fields of cosmetics, health foods, medical appliances and the like; and the preparation method of the collagen peptide has the advantages of being low in input, simple in operation, excellent in protein degradation effect and high in fish skin collagen peptide yield.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a deep-sea fish skin collagen peptide. Background technique [0002] In recent years, with the improvement of living standards, the diversity and practicability of people's demand for aquatic resources have gradually increased, thus promoting the rapid development of my country's aquatic product processing industry. However, with the rapid development of the aquatic product processing industry, a large amount of fish leftovers, such as fish skin, fish scales, viscera, fish heads, etc., will be produced. Most of the feed is discarded, which not only causes a great waste of resources, but also causes environmental pollution. Therefore, how to efficiently utilize the nutritionally active substances in fish scraps has attracted great attention from scientific researchers at home and abroad. Among them, the main waste fish skin is rich in collagen, and collagen can account for 60-60% of ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/34
CPCC12P21/06C07K14/78
Inventor 全盈园
Owner 金华市铁骑士生物科技有限公司
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