A method for recovering sweet tea polyphenols from sweet tea flocculation slag after extracting rubusoside

Abstract

A method for recovering sweet tea polyphenols from sweet tea flocculation slag after rubusoside extraction, comprising the following steps: (1) acid dissolution: adding an acid solution to the sweet tea flocculation slag after extraction of rubusoside, stirring until the flocculation slag dissolves, Centrifuge and filter to obtain filtrate; (2) Macroporous adsorption resin enrichment: enrich the filtrate on a macroporous adsorption resin column, wash with water, elute with organic solvent, concentrate the eluate under reduced pressure and recover the solvent to obtain a concentrated solution; (3) Alumina refining: put the concentrated solution on the alumina chromatography column, wash with water, gradient elution, concentrate the eluate under reduced pressure and recover the eluent, dry in vacuum at low temperature to obtain sweet tea polyphenol products. The purity of the sweet tea polyphenol product obtained by the method of the present invention is ≥96%, and the yield is ≥90%. Not only wastes are reused, cost is saved, but also environmental pollution is reduced; the method of the present invention has short extraction time, simple operation process, energy consumption, The cost is low, and continuous mass production can be realized.

Description

technical field [0001] The invention relates to a method for recovering sweet tea polyphenols, in particular to a method for recovering sweet tea polyphenols from sweet tea flocculation slag after extracting rubusoside. Background technique [0002] Rubus is a perennial deciduous shrub belonging to the family Rosaceae, Rubus. Studies have shown that sweet tea polyphenols have good biological activity, anti-inflammatory, anti-oxidation, anti-allergic and anti-aging effects, and have a good application prospect. Usually, in the process of rubusoside production, sweet tea polyphenols are discharged as waste. On the one hand, it causes a huge waste of resources and also pollutes the environment. Even if it is recycled, the cost is high and the product purity is low. [0003] CN105012359A discloses a method for recovering sweet tea polyphenols. The sweet tea leaves are cleaned, greened, quick-frozen, microwave-thawed 3 times, steam-exploded, extracted with an organic solvent, tr...

AI Technical Summary

Problems solved by technology

The disadvantage of this method is: the pretreatment process of sweet tea leaves is more complicated, and the extract is directly passed through the D-101 macroporous adsorption resin, rubusoside and sweet tea polyphenols will be adsorbed at the same time, and most of the sweet tea polyphenols will be adsorbed on the resin , the amount of sweet tea polyphenols in the effluent is less, so a large amount of resources of sweet tea polyphenols will be wasted

Although this method can obtain high-purity sweet tea polyphenols, the cost of alcohol extraction is high, and when using macroporous adsorption resin to absorb sweet tea polyphenols, it will also enrich rubusoside, so the polyphenol content cannot reach more than 90%.